Promoter methylation and expression changes of CDH1 and P16 genes in invasive breast cancer and adjacent normal breast tissue.

We studied the promoter methylation status and expression levels of P16 and CDH1 genes in breast cancer and their adjacent normal tissues with normal control breast tissues, to correlate with their histopathological parameters. Hundred twenty four samples (tumor and adjacent nonmalignant tissues) from 62 breast cancer patients and 4 normal control breast tissues were included in the study. We used methylation specific PCR to evaluate methylation status and quantitative RT-PCR to measure the gene expression levels. Methylation incidence of P16 gene and CDH1 gene in tumor tissues were 24.2 % and 33.9 %, respectively. CDH1 and P16 gene were not methylated in normal control tissues. CDH1 underexpression is found to be significant in correlation with advanced stage, histologic type, high tumor grade and lymph node involvement. P16 expression is found not to be significantly related with any histopathological parameters. But 60% of cases which overexpresses P16 were estrogen negative, and 40% of them were histologic grade 3. Both P16 and CDH1 had different expression levels in tumor tissues compared to the adjacent normal tissues and in adjacent normal tissues compared to the normal non-tumor tissues.

Inflammatory myofibroblastic tumour (IMT) of the liver: a report of two cases.

Inflammatory myofibroblastic tumour (IMT) is an uncommon mass lesion composed of myofibroblasts and mixed inflammatory infiltrate that rarely undergoes malignant transformation. Although IMT was originally reported in the lung, it is now recognised that it can occur in a variety of organs. Hepatic localisation of IMT is less frequent. Here we report two cases of IMT in the liver. They underwent hepatic resections with the diagnosis of FNH and Klatskin tumour. The pathological diagnosis of the tumour was IMT. We conclude that in the management of IMT in the liver, surgical excision is the primary choice, in order to obtain a definitive diagnosis as well as to relieve symptoms, and strict follow-up after surgery is required for the timely detection of recurrence.

Inflammatory Myofibroblasts Tumour (IMT) of the Liver: A Report of Two Cases.

Inflammatory myofibroblastic tumour (IMT) is an uncommon mass lesion composed of myofibroblasts and mixed inflammatory infiltrate that rarely undergoes malignant transformation. Although IMT was originally reported in the lung, it is now recognised that it can occur in a variety of organs. Hepatic localisation of IMT is less frequent. Here we report two cases of IMT in the liver. They underwent hepatic resections with the diagnosis of FNH and Klatskin tumour. The pathological diagnosis of the tumour was IMT. We conclude that in the management of IMT in the liver, surgical excision is the primary choice, in order to obtain a definitive diagnosis as well as to relieve symptoms, and strict follow-up after surgery is required for the timely detection of recurrence.

Synchronous appearance of male breast cancer and pancreatic cancer 15 years after the diagnosis of testicular cancer--report of a case.

The frequency of new neoplastic diseases among patients cured of testicular cancer is higher than in normal population. For these patients, synchronous occurrence of multiple neoplasms is not common. Also, less than 1% of all cases of breast cancer occur in males. We present herein a case having both breast and concurrent pancreatic cancer after being effectively treated for testicular cancer. To the best of our knowledge, this is the first case of synchronous breast and pancreatic cancer in a male patient following testicular cancer. Second cancer is the most severe long-term complication of chemotherapy or radiotherapy for patients with testicular cancer and the possibility of multiple cancers has to be taken into consideration when multiple lesions are present.