[Optical introscopy is a new diagnostic technique in reproductive medicine].

The WHO classification's concept "infertility of unclear genesis" is due to a number of circumstances. On the one hand, this is a preponderance of the subtle forms of diseases, which are a cause of female infertility, including the subclinical forms of small pelvic inflammatory diseases (SPID). On the other hand, this is an imperfection of existing diagnostic methods. Laparoscopy considered to be the gold standard demonstrates a not very high efficiency in diagnosing SPID because of its low sensitivity. In practice, laparoscopic diagnosis of SPID is combined with ultrasound study, computed tomography, and magnetic resonance tomography. This paper proposes to use optical coherent tomography (OCT) in addition to laparoscopy. OCT makes it possible to noninvasively in real time obtain information on the internal structure of biological tissues with a resolution of 10-15 pm at a depth of at least 2 mm. Removable endoscopic probes make OCT compatible with standard endoscopic studies. The use of OCT during laparoscopy yielded optical images of the internal structure of the fallopian tube wall in different conditions: unaltered fallopian tubes; an acute inflammatory process with pronounced changes; minimal manifestations of fallopian tube inflammatory changes. Based on the comparative analysis of OCT data and histological findings, the authors elaborated OCT criteria for health and disease. A blind test indicated the high diagnostic efficacy of the technique. The additional processing of images makes it possible to objectify the data and to automate the optical introscopic technique proposed by the authors.

[Noninvasive estimation of the oxygen state of experimental tumor by diffuse optical spectroscopy].

The potentialities of diffuse optical spectroscopy for the noninvasive estimation of the oxygen state of experimental tumors have been demonstrated. The distribution of total, oxygenated and deoxygenated hemoglobin, as well as the level oxygen saturation of blood have been shown using two tumor models differing in the histological structure and functional characteristics. The results obtained by the optical method have been verified by immunohistochemical examination of tissue specimens with the exogenous hypoxia marker pimonidazole.

[Optic coherent tomography: a new high-resolution technology of visualization of tissue structures. Communication II. Optical images of benign and malignant entities]. / Opticheskaia kogerentnaia tomografiia -- novaia vysokorazreshaiushchaia tekhnologiia vizualizatsii struktury tkanei. Soobshchenie II. Opticheskie izobrazheniia dobrokachestvennykh i zlokachestvennykh sostoianii.

This is the second communication of a series of publications on Russian studies in the field of optical coherent tomography (OCT), the newest noninvasive highly resolving technology of visualization of the structure of biological tissues. By using the investing tissues as an example, this paper demonstrates the universal types of changes in their optical properties. Optimal images permit differentiate benign and malignant processes with a high degree of diagnostic accuracy. Diverse benign processes occurring in the epithelium are detected on the OCT images as changes in its height, the scattering properties and stroke of a basilar membrane. The absence of any structure on the image is the main OCT criterion for malignancy. The diagnostic efficiency of OCT is high in recognizing neoplasia of various mucous membranes: the sensitivity of the technique is 77-98%; its specificity and diagnostic accuracy are 71-96 and 81-87%, respectively.

Diagnostic efficacy of optical coherence tomography in the management of preinvasive and invasive cancer of uterine cervix and vulva.

The purpose of this study was to understand the capabilities and utility of optical coherence tomography (OCT) in characterizing tissue in patients with precancer and cancer of the uterine cervix and vulva. OCT is an optical technique that uses low-coherence interferometer to develop a two-dimensional image of optical scattering from internal tissue microstructure. This study was designed to develop diagnostic criteria. Women undergoing colposcopic evaluation secondary to an abnormal Papanicolaou smear or visualized grossly abnormal vulvar lesion comprised the study population. Under colposcopic visualization, the OCT device was applied to normal regions in all patients and abnormal areas when present, and images were captured. Each subject then underwent multiple directed biopsies. Images were then reviewed and compared with matched histology. A total of 50 women were recruited for the study. Of the 50 patients evaluated, 18 had cervical intraepithelial neoplasia (CIN) II,III, 14 had CIN I, 13 had metaplasia/inflammation, two had invasive squamous cell carcinoma of the cervix, and three had a diagnosis of Paget's disease of the vulva. Analysis of the OCT images showed a repetitive pattern that represented normal squamous epithelium of the cervix in 100% of the normal biopsies. Images of the 18 patients with histologically proven CIN II,III showed an unstructured homogeneous highly backscattering region with fast attenuation of the signal in 16 (89%) of the patients. OCT is a new approach for the early identification of cervix and vulvar malignancies. Using information inherent to the returning photon signals from tissue, early morphological and light-scattering changes can be detected during tumorigenesis. It has the potential to be a true optical biopsy. If diagnostically comparable to a biopsy, then clearly the ability of OCT to provide a point of service diagnosis would serve a significant advantage.

[Optic coherent tomography: a new high-resolution technology of visualization of tissue structures. Part 1. Principle of the technique. Objects of OCT applications and technical decisions for their study]. / Opticheskaia kogerentnaia tomografiia--novaia vysokorazreshaiushchaia tekhnologiia vizualizatsii struktury tkanei. Soobshchenie 1. Printsip metoda. Ob"ekty prilozheniia OKT i tekhnicheskie resheniia dlia ikh issledovaniia.

The authors present a series of three papers dedicated to studies made in Russia in the field of optic coherent tomography (OCT), the latest noninvasive high-resolution technology of visualization of the structure of biological tissues. A group of medical specialists of different disciplines has been examined over 2,000 patients for 8 years of the use of an original class of the Russian OCT devices developed at the Institute of Applied Physics, Russian Academy of Sciences, and awarded a RF State Prize in the field of science and technology in 1999. The first paper discusses the basic aspects of OCT, the objects of study, and technical decisions for the clinical application of the technique. The paper shows a place of OCT, whose resolving capacity is close to the cellular level (approximately 10-15 microns), among classical methods for imaging biological tissues. The optical images of different types of normal tissues and the method of their identification are demonstrated. It has been found that different optical properties of investing tissues and dentin permit their tomographic differentiation by showing their regular bedded structure. The tomographic pattern of investing tissues is affected by their specific features, such as the type of the lining epithelium, its keratosic processes, and the architecture of the basilar membrane.