Asunto(s)
Mapeo Cromosómico , Cisteína Endopeptidasas , Endopeptidasas/genética , Animales , Bovinos , Genes MHC Clase II , Humanos , Ratones , Alineación de SecuenciaAsunto(s)
Interleucina-6/genética , Polimorfismo de Longitud del Fragmento de Restricción , Porcinos/genética , Animales , Secuencia de Bases , ADN/química , Desoxirribonucleasa HpaII , Desoxirribonucleasas de Localización Especificada Tipo II , Femenino , Masculino , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , LinajeRESUMEN
A five-point linkage map has been established between the loci encoding liver/bone/kidney alkaline phosphatase (ALPL), enolase 1-alpha (ENO1), glucose-phosphate isomerase (GPI), phosphogluconate dehydrogenase (PGD), and transforming growth factor beta 1 (TGFB1) in swine. Linkage analysis was performed using the Meishan x Yorkshire three-generation reference pedigree at the University of Illinois (n = 91). Previously ENO1, GPI, PGD, and TGFB1 were mapped to porcine chromosome 6q by in situ hybridization but the linkage relations of TGFB1 and ENO1 with other loci in this group were not investigated. Based on mapping data from human chromosomes 1 and 19 and mouse chromosomes 4 and 7, it was postulated that ALPL should reside among or near these loci. Restriction fragment length polymorphisms were identified for ALPL, ENO1, and TGFB1. GPI (EC 5.3.1.9) and PGD (EC 1.1.1.44) phenotypes were determined by agarose gel electrophoresis of isozymes. Marker data were analyzed using the MLINK (two locus) and ILINK (multilocus) programs from LINKAGE (version 5.10). The most likely locus order between GPI-TGFB1-(PGD-ENO1)-ALPL with recombination rates of 0.049, 0.044, 0.000, and 0.156, respectively, could not be significantly determined. The maximum five-point lod score was the same to four decimal places irrespective of the order of ENO1 and PGD. This indicates that ENO1 and PGD are very closely linked and that ALPL is located telomeric to the established linkage group on pig chromosome 6.
Asunto(s)
Fosfatasa Alcalina/genética , Mapeo Cromosómico , Ligamiento Genético , Glucosa-6-Fosfato Isomerasa/genética , Isoenzimas/genética , Fosfogluconato Deshidrogenasa/genética , Porcinos/genética , Factor de Crecimiento Transformador beta/genética , Animales , Cromosomas Humanos Par 1 , Cromosomas Humanos Par 19 , Cruzamientos Genéticos , Enzimas de Restricción del ADN , Femenino , Humanos , Hibridación in Situ , Masculino , Linaje , Polimorfismo de Longitud del Fragmento de Restricción , Mapeo RestrictivoRESUMEN
Pituitary response to exogenous gonadotrophin-releasing hormone (GnRH) was examined at a late stage of pregnancy in 10 Booroola-Assaf ewes heterozygous at the FecB locus (FecBFec+) and in 11 Assaf ewes that were non-carriers (Fec+Fec+). Basal plasma luteinizing hormone (LH) and follicle stimulating hormone (FSH) concentrations were similar in the two genotypes. Administration of 100 micrograms of GnRH resulted in a significant increase (P < 0.05) in plasma LH and FSH concentrations in most of the ewes treated. Maximal responses were observed 105-135 min after GnRH treatment. Pituitary responses to GnRH were significantly lower (P < 0.05) in Booroola-Assaf than in Assaf ewes. The decreased pituitary responsiveness observed in FecB gene carriers compared with Fec+Fec+ ewes might be due to differences in the concentrations of ovarian or uterine hormones modulating the release of gonadotrophin. The results suggest that FecB-specific differences can be observed at the pituitary level.