Completely non-invasive cell manipulation in lens-integrated microfluidic devices by single-fiber optical tweezers.

In a fiber-based optical tweezer system, it is a common practice to insert the fiber probe into the sample solution to perform the tweezer function. Such a configuration of the fiber probe may lead to unwanted contamination and/or damage to the sample system and is thus potentially invasive. Here, we propose a completely non-invasive method for cell manipulation by combining a microcapillary microfluidic device and an optical fiber tweezer. We demonstrate that Chlorella cells inside the microcapillary channel can be successfully trapped and manipulated by an optical fiber probe located outside of the microcapillary, thus making the process completely non-invasive. The fiber does not even invade the sample solution. To our knowledge, this is the first report of such a method. The speed of stable manipulation can reach the 7 µm/s scale. We found that the curved walls of the microcapillaries worked like a lens, which helped to boost the light focusing and trapping efficiency. Numerical simulation of optical forces under medium settings reveals that the optical forces can be enhanced by up to 1.44 times, and the optical forces can change direction under certain conditions.

Combined Feature Extraction and Random Forest for Laser Self-Mixing Vibration Measurement without Determining Feedback Intensity.

To measure the vibration of a target by laser self-mixing interference (SMI), we propose a method that combines feature extraction and random forest (RF) without determining the feedback strength (C). First, the temporal, spectral, and statistical features of the SMI signal are extracted to characterize the original SMI signal. Secondly, these interpretable features are fed into the pretrained RF model to directly predict the amplitude and frequency (A and f) of the vibrating target, recovering the periodic vibration of the target. The results show that the combination of RF and feature extraction yields a fit of more than 0.94 for simple and quick measurement of A and f of unsmooth planar vibrations, regardless of the feedback intensity and the misalignment of the retromirror. Without a complex optical stage, this method can quickly recover arbitrary periodic vibrations from SMI signals without C, which provides a novel method for quickly implementing vibration measurements.