RESUMEN
BACKGROUND: : Circular RNAs (circRNAs) have been shown to be extensively involved in preeclampsia progression. At present, the role of circ_0007445 in preeclampsia progression is not clear. METHODS: A total of 30 preeclampsia patients and 30 normal pregnant women were recruited in our study. The function of trophoblast cells was explored to clarify the role and mechanism of circ_0007445 on the preeclampsia progression. The expression of circ_0007445, microRNA (miR)-4432 and high temperature requirement A1 (HTRA1) was analyzed by quantitative real-time PCR. The proliferation, migration and invasion of trophoblast cells were determined by cell counting kit 8 assay, EdU assay, colony formation assay, flow cytometry, and transwell assay. Protein expression was examined by western blot analysis. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay were used to assess RNA interaction relationships. RESULTS: Our data suggested that circ_0007445 had increased expression in preeclampsia patients. Knockdown of circ_0007445 enhanced trophoblast cell proliferation, migration and invasion. MiR-4432 was lowly expressed in preeclampsia patients, and it could be sponged by circ_0007445. MiR-4432 inhibitor overturned the promotion effects of circ_0007445 knockdown on trophoblast cell functions. HTRA1 was highly expressed in preeclampsia patients, and it could be targeted by miR-4432. HTRA1 overexpression could also reverse the proliferation, migration and invasion of trophoblast cells promoted by miR-4432 mimic. In addition, circ_0007445 positively regulated HTRA1 through targeting miR-4432. CONCLUSION: :Our results suggested that circ_0007445 facilitated the development of preeclampsia by suppressing trophoblast cell function through miR-4432/HTRA1 axis.
Asunto(s)
Movimiento Celular , Proliferación Celular , Serina Peptidasa A1 que Requiere Temperaturas Altas , MicroARNs , Preeclampsia , ARN Circular , Trofoblastos , Humanos , Femenino , Preeclampsia/genética , Preeclampsia/metabolismo , MicroARNs/genética , MicroARNs/metabolismo , Trofoblastos/metabolismo , Serina Peptidasa A1 que Requiere Temperaturas Altas/genética , Serina Peptidasa A1 que Requiere Temperaturas Altas/metabolismo , ARN Circular/genética , ARN Circular/metabolismo , Embarazo , AdultoRESUMEN
Meteorological parameters such as relative humidity have a significant impact on the precision of PM2.5 measurement instruments based on light scattering. Instead of adding meteorological sensors or dehumidification devices used widely in commercial PM2.5 measurement instruments, a novel particle sensor based on multi-angle light scattering and data fusion is proposed to eliminate the effect of meteorological factors. Three photodiodes are employed to collect the scattered light flux at three distinct angles. Weather index is defined as the ratio of scattered light fluxes collected at the 40° and 55° angles, which can be used to distinguish the mass median diameter variation caused by different meteorological parameters. Simulations based on Lorenz-Mie theory and field experiments establish the feasibility of this scheme. Experimental results indicate that mass median diameter has less effect on the photodiode at the 55° angle in comparison with photodiodes at the 40° angle and 140° angle. After correction using the weather index, the photodiode at the 40° angle yielded the best results followed by photodiodes at the 55° angle and the 140° angle.
