Triazophos-induced oxidative stress and histomorphological changes in ovary of female Wistar rats.

Triazophos (TZ), a non-systemic broad spectrum organophosphate (OP), is being extensively used against a wide range of pests in agricultural practices. The present study was carried out to investigate the toxic effects of triazophos (TZ) in female Wistar rats. Three sub-chronic dose levels of TZ corresponding to 1/10th, 1/20th and 1/40th of LD50 were given for 30 days to adult female Wistar rats through oral intubation. During the treatment period estrous cycle was significantly altered. Activity levels of different oxidative stress (OS) parameters viz. catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were differentially altered in the ovary of treated rats. Estradiol levels were significantly high while progesterone levels were significantly reduced in plasma of 1/10th and 1/20th of LD50 TZ-treated rats. Histomorphological studies of ovary revealed increased follicular atresia and increased ovarian surface epithelial height in 1/10th and 1/20th of LD50 TZ-treated rats. Enhanced apoptosis and necrosis were also observed in ovarian granulosa cells at dose-dependent manner. Results infer that TZ exposure may lead to the number of pathophysiological conditions in female rats and severity increases at high doses.

Triazophos induced oxidative stress and histomorphological changes in liver and kidney of female albino rats.

Organophosphate (OP) pesticides toxic manifestations have been associated with enhanced generation of reactive oxygen species (ROS) which culminates into oxidative stress (OS). Triazophos (TZ), a broad spectrum OP, was used in the present study to investigate toxic effects on liver and kidney of female albino rats at three sub-chronic dose levels of 1/10th, 1/20th and 1/40th of LD50 for 30 days. Activity levels of different OS parameters viz: catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were differentially altered in liver and kidney of TZ treated rats as compared to control rats. Histomorphological studies further revealed number of abnormalities as infiltration, vacuolization, enlarged sinusoids and necrosis in liver of TZ treated rats, while renal histoarchitecture of 1/10th and 1/20th of LD50 TZ treated rats showed high degeneration of glomeruli. Enhanced apoptosis was also observed in hepatocytes at dose levels of 1/10th and 1/20th of LD50 TZ treated female rats. The results infer that exposure of female albino rats to TZ leads to number of pathophysiological conditions in the liver and kidney at dose dependent manner.