Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
BMC Res Notes ; 15(1): 188, 2022 May 21.
Artículo en Inglés | MEDLINE | ID: mdl-35597992

RESUMEN

OBJECTIVE: Toxoplasma gondii is a ubiquitous parasite of medical and veterinary importance; however, there exists no cure for chronic toxoplasmosis. Metabolic enzymes required for the production and maintenance of tissue cysts represent promising targets for novel therapies. Here, we use reverse genetics to investigate the role of Toxoplasma phosphoglucomutase 1, PGM1, in Toxoplasma growth and cystogenesis. RESULTS: We found that disruption of pgm1 did not significantly affect Toxoplasma intracellular growth and the lytic cycle. pgm1-defective parasites could differentiate into bradyzoites and produced cysts containing amylopectin in vitro. However, cysts produced in the absence of pgm1 were significantly smaller than wildtype. Together, our findings suggest that PGM1 is dispensable for in vitro growth but contributes to optimal Toxoplasma cyst development in vitro, thereby necessitating further investigation into the function of this enzyme in Toxoplasma persistence in its host.


Asunto(s)
Fosfoglucomutasa , Toxoplasma , Toxoplasmosis , Humanos , Fosfoglucomutasa/genética , Fosfoglucomutasa/metabolismo , Toxoplasma/enzimología , Toxoplasma/genética , Toxoplasmosis/parasitología
2.
Microbes Infect ; 22(10): 525-533, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32931908

RESUMEN

Toxoplasma gondii reproduces sexually in felines and asexually in virtually all warm-blooded animals, including humans. This obligate intracellular parasite alternates between biologically distinct developmental stages throughout its complex life cycle. Stage conversion is crucial for T. gondii transmission, persistence, and the maintenance of genetic diversity within the species. Genome-wide comparative transcriptomic studies have contributed invaluable insights into the regulatory gene networks underlying T. gondii development.


Asunto(s)
Regulación del Desarrollo de la Expresión Génica , Estadios del Ciclo de Vida/genética , Toxoplasma/crecimiento & desarrollo , Transcriptoma , Animales , Variación Genética , Humanos , Reproducción , Toxoplasma/genética , Toxoplasmosis/parasitología , Toxoplasmosis/transmisión
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA
...