Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Más filtros











Intervalo de año de publicación
1.
Biol Res ; 47: 52, 2014 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-25418358

RESUMEN

BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101. CONCLUSIONS: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.


Asunto(s)
Proteínas de la Membrana/metabolismo , Epitelio Seminífero/metabolismo , Seminoma/metabolismo , Neoplasias Testiculares/metabolismo , Western Blotting , Diferenciación Celular , Epitelio/metabolismo , Femenino , Tracto Gastrointestinal/metabolismo , Humanos , Inmunohistoquímica , Tejido Linfoide/metabolismo , Masculino , Tejido Nervioso/metabolismo , Especificidad de Órganos/fisiología , Ovario/metabolismo , Epitelio Seminífero/patología , Seminoma/patología , Maduración del Esperma/fisiología , Espermatozoides/crecimiento & desarrollo , Neoplasias Testiculares/patología , Testículo/metabolismo , Testículo/patología
2.
Biol. Res ; 47: 1-6, 2014. ilus
Artículo en Inglés | LILACS | ID: biblio-950748

RESUMEN

BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101. CONCLUSIONS: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.


Asunto(s)
Humanos , Masculino , Femenino , Epitelio Seminífero/metabolismo , Neoplasias Testiculares/metabolismo , Seminoma/metabolismo , Proteínas de la Membrana/metabolismo , Especificidad de Órganos/fisiología , Ovario/metabolismo , Epitelio Seminífero/patología , Maduración del Esperma/fisiología , Espermatozoides/crecimiento & desarrollo , Neoplasias Testiculares/patología , Testículo/metabolismo , Testículo/patología , Inmunohistoquímica , Diferenciación Celular , Western Blotting , Seminoma/patología , Tracto Gastrointestinal/metabolismo , Epitelio/metabolismo , Tejido Linfoide/metabolismo , Tejido Nervioso/metabolismo
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA