[Digestive enzyme activity during immobilization stress and its pharmacologic correction with adrenoblockaders]. / Aktivnost' pishchevaritel'nykh fermentov pri immobilizatsionnom stresse i ego farmakologicheskoi korrektsii adrenoblokatorami.

The change from relative digestive rest to the phase of digestion is characterized by various dynamics of the activity of enterokinase, amylase, and intestinal isoenzyme of alkaline phosphatase. The effect of the food stimulus in 3-hour immobilization can be considered antistress because the activity of the intestinal enzymes is almost the same as that in intact animals. It was found that the intestinal digestive enzymes become adapted to repeated short-term immobilization stress. Administration of alpha- and beta-adrenergic blocking agents changes the response of the digestive enzymes to stress by lowering their activity.

[Functional biochemical changes and their pharmacologic correction by pirroksan in renal ischemia]. / Funktsional'no-biokhimicheskie izmeneniia i ikh farmakologicheskaia korrektsiia pirroksanom pri ishemii pochek.

Bilateral one-hour ischemia of the kidneys in rats causes essential metabolic shifts in the renal tissues, and diminished intensity of the energetic status as a result of which the intactness of the cell membranes and the main functions of the nephron are disturbed. Pharmacological correction of postischemic disorders by the alpha-adrenergic blocking agent pyrroxane increases the activity of oxidative processes and intensifies the glycolysis rate. Improvement of the energy provision of the nephron function occurs in parallel with restoration of the diuresis level and processes of filtration and reabsorption.

[Effect of pyruvate on metabolic processes in erythrocytes after acute massive blood loss]. / Vliianie piruvata na obmennye protsessy v éritrotsitakh posle ostroi massivnoi poteri krovi.

It is shown in the in vitro experiments that addition into the incubation medium of 10 mumol pyruvate is accompanied by the inhibition of the lactate dehydrogenase activity. The level of 2.3-biphosphoglycerate, ATP, lactate and hemoglobin increases by 92%, 265%, 93% and 56%, respectively. The acute massive haemorrhage against a background of infusion of 0.065% solution of pyruvate does not evoke a decrease in the hemoglobin level, which is observed after infusion of the physiological solution. The value of hematocrit proved to be higher to some extent. The 2,3-biphosphoglycerate level and the activity of glucoso-6-phosphate dehydrogenase are not changed in comparison with the results of intact rats. The glutathione reductase activity rises by 38%. The lactate level diminishes by 34%. Pyruvate is established to exert a therapeutic effect in acute massive haemorrhage. The survival rate of rats after infusion of pyruvate increases by 65%.

[Metabolic changes in blood cells in various forms of ischemic heart disease]. / Metabolicheskie izmeneniia v kletkakh krovi pri razlichnykh formakh ishemicheskoi bolezni serdtsa.

In various clinical types of heart ischemic disease (stenocardia of stress and rest, large scale- and small scale myocardial infarction) alterations in activity of glycolysis and Krebs cycle enzymes as measured in leukocytes were especially distinct under conditions of large scale myocardial infarction as compared with the small scale infarction and stenocardia. Energy metabolism of leukocytes in heart ischemic disease was altered due to functional impairment of these cells, of the cellular immunity state as well as to the alteration in the activity of hydrolytic enzymes of leukocytes accumulating in the inflammatory infiltrate in myocardium. In the ischemic disease of heart the gas transport functions of erythrocytes were altered; the cells, exhibiting high resistance and elevated activity of the pentose-phosphate shunt, were found in circulation. The metabolic alterations in erythrocytes affected the rheological properties of blood. Estimation of enzymatic activity in blood cells is important for diagnosis of the heart ischemic disease of various types.

[Metabolic consequences of kidney normothermic ischemia]. / Metabolicheskie posledstviia normotermicheskoi ishemin pochek.

Normothermic ischemia of kidney within 1 hr caused critical impairments in metabolic reactions of nephrons. Activities of glucose-6-phosphate and lactate dehydrogenases in erythrocytes may characterize the functional state of kidney tissue in ischemia. Amount of 2,3-glucose diphosphate in erythrocytes correlated with the diuresis level. Kidney tissue ischemia led to alteration in the ratio of young and old erythrocyte populations in circulation. Alterations in activity of glucose-6-phosphate and lactate dehydrogenases in urine occurred due to both an increase in kidney clearance and the impairment of kidney tissue.

[Metabolic response of leukocytes as an index of the individual response of an animal to stress and traumatic shock]. / Metabolicheskii otvet leikotsitov kak pokazatel' individual'noi reakstii zhivotnogo na stress i shokogennuiu travmu.

A relationship has been established between varying metabolic response of leukocytes to immobilization stress and severity of the shock progress. Initiation of the shock is accompanied by different response of animal leukocytes that correlates with the severity of the subsequent process. Formation of the resistance to shock is associated with an active glucose involvement into energy and plastic metabolic reactions, activation of the substrate-enzymic systems of glycolysis, pentose shunt, terminal stage of the respiratory chain, decreased rate of succinte dehydrogenation.