[Experimentally determined safety and immunological activity of vaccine based on antigens isolated from Pseudomonas aeruginosa in medium K-4].

The safety and immunological activity of P. aeruginosa vaccine were experimentally evaluated. The vaccine was prepared on the basis of the antigens of P. aeruginosa extracellular slime which was accumulated in medium K-4, obtained with the use of original technology. The immunization of animals with P. aeruginosa vaccine induced the synthesis of antibodies. The introduction of the vaccine in 2 or 3 injections resulted in a high level of antibody formation, differing with the use of various strains. Hyperimmune sera, obtained by the multiple immunization of rabbits with P. aeruginosa vaccine, ensured high protection of mice from P. aeruginosa infection. The vaccine proved to be safe when evaluated in experiments of acute and chronic toxicity, made on laboratory animals.

[Pseudomonas aeruginosa vaccine on the basis of antigens isolated from the supernatant of culture media K-4]. / Sinegnoinaia vaktsina na osnove antigenov, vydelennykh iz supernatanta sredy kul'tivirovaniia K-4.

The possibility of using experimental culture medium K-4 prepared on the basis of casein hydrolysate peptides with the isoelectric point 4.1 for obtaining antigens from P. aeruginosa strains was evaluated. Two antigenic fractions were isolated from the culture fluid containing extracellular slime. The study of the toxicity of the antigenic preparations revealed that one of these fractions had low toxicity for mice (the second antigenic fraction was highly toxic). The former P. aeruginosa antigenic fraction was used for obtaining pyocyanic vaccine. One vaccination dose of this vaccine contained 0.2 mg of the antigen adsorbed on aluminum hydroxide. Pyocyanic vaccine ensured the active protection of mice challenged with P. aeruginosa homologous and heterologous strains.

[Study on the effect of a biostimulant on the growth and toxigenic function of Clostridium tetani strain Copenhagen-471]. / Izuchenie vliianiia biostimuliatora na rost i toksinoobrazuiushchuiu funktsiiu Clostridium tetani "Kopenhagen-471."

Bakstim, a new biostimulating preparation obtained from the organs of the immune system of animals, was developed. The impact of Bakstim on the growth and toxigenic function of C. tetani production strain Copenhagen-471 was evaluated. The addition of the preparation to Gluzman commercial medium for obtaining tetanus toxoid led to an increase in the yield of bacterial biomass from 1.9 to 4-fold and an increase in the toxoid production from 2 to 2.8-fold. The optimum concentration of this biostimulant ensuring the maximum yield of tetanus toxin from the production culture was determined (1,000 mg/l). Bakstim will supposedly be used as additive to nutrient media for the production of tetanus toxoid.

[Evaluation of the toxicity of a chemical by adsorbed typhoid vaccine by a pharmacological test (hexenal sleep) and by a change in thymus weight in white mice]. / Otsenka toksichnosti khimicheskoi sorbirovannoi briushnotifoznoi vaktsiny po farmakologicheskomu testu (geksenalovyi son) i izmeneniiu massy timusa belykh myshei.

With the wide spread of immunoprophylaxis, investigations aimed at introducing new methods for the control of vaccines are gaining in importance. As indicated by the results of investigations, the reactogenicity of vaccinal preparations for humans may be evaluated, to a certain extent, by the degree of the stress action produced by these preparations and the duration of hexenal narcosis in laboratory animals.