RESUMEN
OBJECTIVE: To compare the functions and complications of forearm basilic vein transposition-arteriovenous fistula (BVT-AVF) created using the no-touch technique with that of conventional radiocephalic arteriovenous fistula (RC-AVF). MATERIALS AND METHODS: The no-touch technique was used to created basilic vein transposition-radial artery fistula in 22 patients. Another 30 patients received surgeries for RC-AVF. The fistula functions and complications were compared between these two groups. RESULTS: The two groups did not differ significantly in the incidence of postoperative bleeding, limb swelling, infection, steal syndrome, fistula thrombosis, fistula aneurysm, fistula flow, fistula maturation time, Kt/v, and fistula median survival. CONCLUSION: Forearm BVT-AVF created by the no-touch technique is a good alternative access for patients in whom the standard arteriovenous fistula cannot be established.
Asunto(s)
Derivación Arteriovenosa Quirúrgica , Antebrazo/irrigación sanguínea , Arteria Radial/cirugía , Venas/cirugía , Derivación Arteriovenosa Quirúrgica/efectos adversos , Derivación Arteriovenosa Quirúrgica/métodos , Derivación Arteriovenosa Quirúrgica/estadística & datos numéricos , Humanos , Hemorragia Posoperatoria/epidemiologíaRESUMEN
This study was conducted to investigate the biological role of periostin in gastric cancer (GC) under hypoxia. Western blot analysis revealed that along with an upregulation of hypoxia-inducible factor-1alpha, there was a time-dependent induction of periostin in MKN-45 cells under hypoxia (2% O2 ), increasing by eightfold as compared to normoxic cells. Pretreatment with 30 µM PD98059, an inhibitor of ERK1/2, significantly reduced hypoxia-stimulated periostin expression (P < 0.01). Periostin knockdown in MKN-45 cells was achieved by specific small interfering RNA (siRNA). The conditioned medium from periostin siRNA-transfected MKN-45 cells induced significantly less (P < 0.01) endothelial tube formation than control siRNA-transfected cells. Additionally, periostin silencing markedly decreased the mRNA expression and secretion of vascular endothelial growth factor (VEGF) in hypoxic MKN-45 cells. Thus, our data suggest that periostin is a hypoxia-response gene and mediates a cross talk between GC and endothelial cells under hypoxia, partially through regulation of the VEGF expression.
Asunto(s)
Moléculas de Adhesión Celular/metabolismo , Proteínas de Neoplasias/metabolismo , Neovascularización Patológica/metabolismo , Neoplasias Gástricas/metabolismo , Factor A de Crecimiento Endotelial Vascular/biosíntesis , Moléculas de Adhesión Celular/genética , Comunicación Celular/efectos de los fármacos , Comunicación Celular/genética , Hipoxia de la Célula/genética , Línea Celular Tumoral , Técnicas de Cocultivo , Flavonoides/farmacología , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Regulación Neoplásica de la Expresión Génica/genética , Técnicas de Silenciamiento del Gen , Células Endoteliales de la Vena Umbilical Humana , Humanos , Proteína Quinasa 1 Activada por Mitógenos/antagonistas & inhibidores , Proteína Quinasa 1 Activada por Mitógenos/genética , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos/antagonistas & inhibidores , Proteína Quinasa 3 Activada por Mitógenos/genética , Proteína Quinasa 3 Activada por Mitógenos/metabolismo , Proteínas de Neoplasias/antagonistas & inhibidores , Proteínas de Neoplasias/genética , Neovascularización Patológica/genética , Neovascularización Patológica/mortalidad , Neovascularización Patológica/patología , Inhibidores de Proteínas Quinasas/farmacología , Neoplasias Gástricas/genética , Neoplasias Gástricas/patología , Factor A de Crecimiento Endotelial Vascular/genéticaRESUMEN
48 patients with fractures of the jaw bones were included in this study. Intermaxillary fixation screws were used for tractions between the upper and lower jaw and to regain occlusion relationship, followed by internal rigid fixation. Two days after reduction of the fractures, intermaxillary tractions were carried out and the fixation screws remained for 2 weeks. All the wounds healed primarily with good occlusion. The wounds for screw placement healed well 3 to 5 days after removal of the screws. Excellent occlusion recovered in 45 patients, while in 3 patients, mild open bite developed which returned to normal after 2 weeks of intermaxillary tractions. It is concluded that intermaxillary fixation screws are time saving and efficient in the management of jaw fractures.
Asunto(s)
Fijación Interna de Fracturas , Técnicas de Fijación de Maxilares , Fracturas Maxilomandibulares/cirugía , Tornillos Óseos , Oclusión Dental , HumanosRESUMEN
The interaction between salicylic acid and bovine serum albumin has been studied by fluorescence spectroscopy. The results show that the quenching mechanism of the combination of bovine serum albumin with salicylic acid is a static quenching procedure, the quenching constant K(sv) is 1.097 x 10(4) (mol x L(-1))(-1), and the equilibrium constant is 7.377 x 10(4). The number of binding sites is 1 and it is a strong one. When the ratio of molar concentration of salicylic acid to bovine serum albumin is lower than 1:1, it binds to Trp residue first but it doesn't result in any microenvironment changes of Trp residue. The binding distance between salicylic acid and bovine serum albumin and the energy transfer efficiency were obtained based on the theory of Förester spectroscopy energy transfer.
Asunto(s)
Ácido Salicílico/química , Albúmina Sérica Bovina/química , Albúmina Sérica/química , Espectrometría de Fluorescencia/métodos , Sitios de Unión , Dicroismo Circular/métodos , Interacciones Farmacológicas , Transferencia de Energía , Fluorescencia , Unión Proteica , Conformación Proteica , Espectroscopía Infrarroja por Transformada de Fourier/métodosRESUMEN
Tobacco peroxidase (TOPI) has been purified by ammonium sulfate fractionation and column chromatography, involving ion exchange on DE-52 cellulose, gelfiltration on Sephadex G-75 and ion exchange on DEAE-sephadex A-50. The specific activity of peroxidase purified was 4,826 U/mg. It has been demonstrated by SDS-PAGE as a single band. Its molecular weight (matrix assisted laser desorption/ionization time of flight mass spectra) and isoelectric point (pI) have been determined to be 21,888.5 and 3.5 respectively. The native enzyme is one of a haemachrome-containing acidic protein and has its soret maximum at 402 nm and alpha and beta bands at 636 nm and 498 nm, respectively. Its characteristic absorption spectra and fluorescence spectra changed in different pH and denaturant. It has its own characteristic absorption spectra and fluorescence spectra.