CTBP2 contributes to cisplatin resistance in lung adenocarcinoma by inhibiting generation of reactive oxygen species.

Background: Cisplatin (CP) is commonly used for the initial treatment of lung adenocarcinoma (LUAD). Resistance to CP has long been recognized as a significant obstacle to achieving improved therapeutic outcomes. Nevertheless, the intricate molecular mechanisms underlying the phenomenon remain incompletely understood. Methods: The present study utilized the University of ALabama at Birmingham CANcer data analysis Portal (UALCAN) and Gene Expression Profiling Interactive Analysis (GEPIA) databases to conduct an analysis of the expression of C-terminal binding protein 2 (CTBP2) in LUAD. The correlation between CTBP2 expression and survival data was investigated by the Kaplan-Meier (K-M) plotter. Subsequently, the roles of CTBP2 in CP resistance were explored by analyzing cell viability, cell apoptosis, reactive oxygen species (ROS), and mitochondrial membrane potential (MMP) in CP-resistant cells (A549/DDP). Results: Our data indicated that the CTBP2 expression in LUAD exhibited a significant increase compared to the non-malignant tissues. CTBP2 overexpression showed a correlation to poor survival. CTBP2 knockdown significantly enhanced cell sensitivity to CP in A549/DDP cells. The underlying mechanism is related to promoting ROS production and decreasing MMP after CP treatment. Conclusions: CTBP2 expression has been identified as a novel biomarker for resistance to CP, and its downregulation has been found to enhance sensitivity to CP. Therefore, CTBP2 can serve as a predictor related to CP resistance and a viable therapeutic target for CP resistance in LUAD.

Therapeutic effects of conjunctival flap covering combined with subconjunctival injection of fluconazole on patients with severe fungal corneal ulcer / 国际眼科杂志(Guoji Yanke Zazhi)

AIM:To analyze the clinical efficacy of conjunctival flap covering combined with subconjunctival injection of fluconazole on patients with severe fungal corneal ulcer.METHODS:Totally 50 cases with severe fungal corneal ulcer were collected in our hospital from January 2012 to December 2015.The cases were randomly divided into observation group and control group,25 cases in each group.All the cases were monocular sick.The control group took single drug treatment,but the observation group were given with conjunctival flap as an extra.The clinical efficacy,best corrected visual acuity and adverse effects rate were observed.And,the relapse of the two groups was followed for 1a.RESULTS:After 1 mo of treatment,the clinical curative effect of the observation group was significantly higher than the control group (P＜ 0.05),and corrected visual acuity of the observation group was higher than the control group (P＜0.05);the adverse reaction rate of the observation group was lower than the control group (P＜0.05).After 1a follow-up,the observation group only had 1 case of recurrence,control group had 8 cases with recurrence,the recurrence rate of the observation group was significantly lower than the control group (P＜0.05).CONCLUSION:The conjunctival flap combined with subconjunctival injection of fluconazole has good application value in severe fungal corneal ulcer patients,can effectively improve the clinical curative effect and the visual acuity,and can effectively reduce the incidence of adverse reactions and recurrence rate.