[Use of different media for growing the producer of the restriction enzyme XBA I]. / Ispol'zovanie razlichnykh sred dlia vyrashchivaniia produtsenta restriktiruiushchego fermenta XBA I.

The possibility of using culture media prepared from local ingredients and intended for growing the producers of restricting enzyme Xba 1 has been demonstrated. The yield of restricting enzyme Xba 1 per g of crude biomass, obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories (USA), has proved to be 4 times greater than that obtained with the use of peptone-yeast medium prepared from local ingredients. At the same time the use of casein-saline medium ensures the yield of the enzyme, similar to that obtained with the use of peptone-yeast medium prepared from ingredients supplied by Difco Laboratories, but with a greater content of nonspecific nucleases.

[Development and experimental study of a dry selective medium for staphylococci]. / Razrabotka i éksperimental'noe izuchenie sukhoi élektivnoi sredy dlia stafilokokkov.

A new dried selective medium for the cultivation of staphylococci has been developed. The medium is based on the mixture of hydrolysin past its expiration date and aminopeptide. To inhibit extraneous microflora, sodium chloride in a high concentration is introduced into the medium. The possibility of using this selective medium in the form of milk-yolk-salt agar for the determination of the pigment formation and lecithinase activity of staphylococci is shown.