RESUMEN
Human leukocyte antigen (HLA)-A, -B, and -DRB1 alleles were typed in 46,915 healthy Taiwanese volunteers recruited for Tzu Chi Taiwan Marrow Donor Registry (TCTMDR). The volunteers were separated into Taiwanese and Taiwanese aborigines. In this study, a total of 51 A, 121 B, and 53 DRB1 alleles were found in the Taiwanese group, and 17 A, 32 B, and 23 DRB1 alleles were identified in the Taiwanese aborigines. Some commonly shared alleles appeared more frequently in one group than in the other. The two haplotypes, among the 20 most frequently observed haplotypes in each group, shared in common by both groups were A*3303-B*5801-DRB1*0301 and A*0207-B*4601-DRB1* 0901. However, both haplotype frequencies in each group were extremely different, indicating the existence of genetic diversity between the two groups. In addition, principal component analysis and clustering results based on high-resolution HLA-A, -B, and -DRB1 alleles indicated that the Taiwanese group was closest to Southern Chinese and reiterated HLA diversity between the Taiwanese and the Taiwanese aborigines. We believe that our findings in this study may provide useful information in search for HLA-matched donors for patients.
Asunto(s)
Antígenos HLA-A/genética , Antígenos HLA-B/genética , Antígenos HLA-DR/genética , Donantes de Tejidos , Alelos , Pueblo Asiatico/genética , Médula Ósea/inmunología , Análisis por Conglomerados , Frecuencia de los Genes , Variación Genética , Antígenos HLA-A/clasificación , Antígenos HLA-B/clasificación , Antígenos HLA-DR/clasificación , Cadenas HLA-DRB1 , Haplotipos , Humanos , Análisis de Componente Principal , Sistema de Registros , TaiwánRESUMEN
Using the maximum-likelihood method with the expectation-maximization (EM) algorithm of PYPOP, high-resolution human leukocyte antigen (HLA) three-locus haplotypes (HLA-A, -B, and -C; HLA-A, -B, and -DRB1) and four-locus haplotypes (HLA-A, -B, -C, and -DRB1) were determined. Linkage disequilibrium of high-resolution HLA-B and -C alleles and HLA-DRB1 and -DQB1 alleles was also calculated. Comparison of the Taiwanese haplotypes and haplotypes from donors in the Chinese Han population, the Asia Pacific Islander ethnic category of the NMDP (National Marrow Donor Program), and the Taiwanese cord blood units demonstrated similarities and dissimilarities among the four populations. HLA allele frequencies of our study suggested that the Taiwanese have a relative population relationship with the southern Han Chinese with regard to HLA. Our results also indicated that the Taiwanese population exhibits genetic proximity with Asian Americans with regard to HLA-A and -DRB1 but not HLA-B.
