Thiourethane-functionalized fillers: biological properties and degradation resistance

Abstract This study determined the effect of thiourethane-functionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutans colonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry.

Thiourethane-functionalized fillers: biological properties and degradation resistance.

This study determined the effect of thiourethane-functionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutans colonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry.

Plano de ação para prevenção da ocorrência de gravidez na adolescência na área da ESF Wandy de Moraes Silva - Itaguara/MG

Segundo a Organização Mundial de Saúde denomina-se gravidez na adolescência a gestação ocorrida em jovens de 10 a 20 anos (exclusive) que se encontram, portanto, em pleno desenvolvimento dessa fase da vida - a adolescência. Esse tipo de gravidez em geral não foi planejada, nem desejada e acontece em meio a relacionamentos sem estabilidade. A gravidez precoce é uma das ocorrências mais preocupantes relacionadas à sexualidade da adolescência, com sérias conseqüências para a vida dos adolescentes envolvidos, de seus filhos que nascerão ou não e de suas famílias. Este estudo tem o objetivo de elaborar um plano de ação para prevenção da ocorrência de gravidez na adolescência na área da ESF Wandy de Moraes Silva-Itaguara/MG o qual compreende os seguintes passos: definição do problema; priorização do problema; descrição do problema priorizado; explicação do problema; selecionação dos nós críticos; desenho das operações; identificação dos recursos críticos; análise de viabilidade do plano de ação; elaboração do plano operativo e gestão do plano. O plano de ação realizado pela ESF Wandy de Moraes Silva é aplicável, pois, possui todos os recursos necessários para sua realização e superação dos nós críticos que foram identificados. Isso será possível por meio da execução de projetos intersetoriais.