RESUMEN
We report here on a new amphiphilic homopolymer that binds noncovalently to proteins. This polymer not only binds to the target protein chymotrypsin with submicromolar affinity but also stabilizes the native structure of the protein. Since the polymer-protein binding process is based on electrostatic interaction, the bound protein can be released from the polymer surface and reactivated either by increasing the ionic strength or by adding complementary cationic surfactants. The electrostatic binding of polymer to the protein results in a marked change in the substrate specificity of chymotrypsin.
Asunto(s)
Quimotripsina/química , Polímeros/química , Animales , Bovinos , Quimotripsina/antagonistas & inhibidores , Quimotripsina/metabolismo , Dicroismo Circular , Interacciones Hidrofóbicas e Hidrofílicas , Cinética , Micelas , Conformación Molecular , Fenilalanina/análogos & derivados , Fenilalanina/química , Fenilalanina/metabolismo , Polímeros/metabolismo , Unión Proteica , Espectrometría de Fluorescencia , Especificidad por Sustrato , Propiedades de SuperficieRESUMEN
Negatively charged gold nanoparticles featuring 2-(10-mercapto-decyl)-malonic acid were synthesized using the Murray place-displacement reaction. These water-soluble malonate gold mixed monolayer protected clusters (MMPCs) effectively bind and inhibit chymotrypsin based on complementary electrostatic surface recognition. The effect of increasing ionic strength on inhibition was also studied. It was observed that addition of high ionic strength solutions to protein-nanoparticle complexes show almost complete restoration of protein activity. The conformational change of chymotrypsin upon binding to the MMPC was investigated using fluorescence spectrometry and circular dichroism, thus correlating structural changes with enzyme activity.
Asunto(s)
Quimotripsina/antagonistas & inhibidores , Quimotripsina/química , Oro/química , Malonatos/química , Nanoestructuras/química , Compuestos de Sulfhidrilo/química , Animales , Aniones/química , Sitios de Unión , Bovinos , Dicroismo Circular/métodos , Malonatos/síntesis química , Estructura Molecular , Concentración Osmolar , Páncreas/enzimología , Sensibilidad y Especificidad , Solubilidad , Espectrometría de Fluorescencia/métodos , Electricidad Estática , Relación Estructura-Actividad , Compuestos de Sulfhidrilo/síntesis química , Propiedades de Superficie , Agua/químicaRESUMEN
Positively charged trimethylammonium-functionalized mixed monolayer protected clusters (MMPCs) of different chain lengths (C(8) and C(11)) have been used to bind beta-galactosidase through complementary electrostatic interactions, resulting in complete enzyme inhibition. This inhibition can be reversed in vitro by intracellular concentrations of glutathione (GSH), the main thiol component of the cell. The restoration of activity depends on the chain length of the monolayer. The activity of enzyme bound to particles with C(8) monolayer was completely restored by intracellular concentrations (1-10 mM) of GSH; however, little or no release was observed at extracellular GSH concentrations. In contrast, no restoration was observed for enzyme bound to the C(11) particles at any of the concentrations studied. Taken together, these studies demonstrate that the GSH-mediated release of enzymes bound to MMPCs can be tuned through the structure of the monolayer, a significant tool for protein and drug delivery applications.
Asunto(s)
Glutatión/farmacología , Compuestos de Amonio Cuaternario/farmacología , beta-Galactosidasa/antagonistas & inhibidores , beta-Galactosidasa/metabolismo , Electroforesis en Gel de Agar , Activación Enzimática , Inhibidores Enzimáticos/química , Inhibidores Enzimáticos/farmacología , Glutatión/química , Glutatión/metabolismo , Nanoestructuras , Compuestos de Amonio Cuaternario/química , Compuestos de Amonio Cuaternario/metabolismoRESUMEN
alpha-Helices are important structural elements in proteins. To provide a scaffold for the facial recognition of peptides, we have explored the interaction of cationic mixed monolayer protected clusters (MMPCs) with a tetra-aspartate peptide in water. In these studies, substantial enhancement of peptide helicity was observed upon addition of the MMPC. Significantly, this stabilization increased with time, demonstrating templation of the monolayer to the peptide helix.
Asunto(s)
Nanotecnología/métodos , Péptidos/química , Receptores de Péptidos/química , Oro/química , Tamaño de la Partícula , Estructura Secundaria de Proteína , Espectrometría de FluorescenciaRESUMEN
Negatively charged carboxylate-functionalized mixed monolayer protected clusters (MMPCs) effectively bind and inhibit alpha-chymotrypsin based on complementary electrostatic surface recognition. We demonstrate that this binding can be disrupted by varying the ionic strength of the medium. Enzyme activity in the presence of MMPCs increases from 5% to 97% of native activity as salt concentration is increased from 0 to 1.5 M. Variation of ionic strengths after complete binding over 13 h results only in a modest restoration of enzymatic activity (< 35%). The conformation of chymotrypsin was characterized using circular dichroism and fluorescence spectroscopy, correlating structure with enzymatic activity. This work provides a useful insight of the electrostatic influence on protein--MMPC interactions and can be applied toward MMPC-based controlled release of proteins in vivo.
Asunto(s)
Quimotripsina/química , Nanoestructuras/química , Animales , Ácidos Carboxílicos , Bovinos , Quimotripsina/metabolismo , Oro , Concentración Osmolar , Unión Proteica , Conformación Proteica , Electricidad EstáticaRESUMEN
Anionically functionalized amphiphilic nanoparticles efficiently inhibit chymotrypsin through electrostatic binding followed by protein denaturation. We demonstrate the ability to disrupt this "irreversible" inhibition of chymotrypsin through modification of the nanoparticle surface using cationic surfactants. Up to 50% of original chymotrypsin activity is rescued upon long-chain surfactant addition. Dynamic light-scattering studies demonstrate that chymotrypsin is released from the nanoparticle surface. The conformation of the rescued chymotrypsin was characterized by fluorescence and fluorescence anisotropy, indicating that chymotrypsin regains a high degree of native structure upon surfactant addition.
Asunto(s)
Ácidos Carboxílicos/química , Quimotripsina/antagonistas & inhibidores , Oro/química , Polarización de Fluorescencia , Nanotecnología , Tamaño de la Partícula , Desnaturalización Proteica , Electricidad Estática , Propiedades de Superficie , Tensoactivos/químicaRESUMEN
Efficient binding of biomacromolecular surfaces by synthetic systems requires the effective presentation of complementary elements over large surface areas. We demonstrate here the use of mixed monolayer protected gold clusters (MMPCs) as scaffolds for the binding and inhibition of chymotrypsin. In these studies anionically functionalized amphiphilic MMPCs were shown to inhibit chymotrypsin through a two-stage mechanism featuring fast reversible inhibition followed by a slower irreversible process. This interaction is very efficient, with a K(i)(app) = 10.4 +/- 1.3 nM. The MMPC-protein complex was characterized by CD, demonstrating an almost complete denaturation of the enzyme over time. Dynamic light scattering studies confirm that inhibition proceeds without substantial MMPC aggregation. The electrostatic nature of the engineered interactions provides a level of selectivity: little or no inhibition of function was observed with elastase, beta-galactosidase, or cellular retinoic acid binding protein.
Asunto(s)
Quimotripsina/antagonistas & inhibidores , Quimotripsina/metabolismo , Aniones , Cationes , Quimotripsina/química , Dicroismo Circular , Relación Dosis-Respuesta a Droga , Oro/química , Concentración de Iones de Hidrógeno , Cinética , Luz , Modelos Químicos , Modelos Moleculares , Nanotecnología , Unión Proteica , Dispersión de Radiación , Factores de TiempoRESUMEN
Mixed monolayer protected gold clusters (MMPCs) functionalized with quaternary ammonium chains efficiently transfect mammalian cell cultures, as determined through beta-galactosidase transfer and activity. The success of these transfection assemblies depended on several variables, including the ratio of DNA to nanoparticle during the incubation period, the number of charged substituents in the monolayer core, and the hydrophobic packing surrounding these amines. Complexes of MMPCs and plasmid DNA formed at w/w ratios of 30 were most effective in promoting transfection of 293T cells in the presence of 10% serum and 100 microM chloroquine. The most efficient nanoparticle studied (MMPC 7) was approximately 8-fold more effective than 60 kDa polyethylenimine, a widely used transfection agent.