Vitamin D regulates microbiome-dependent cancer immunity.

A role for vitamin D in immune modulation and in cancer has been suggested. In this work, we report that mice with increased availability of vitamin D display greater immune-dependent resistance to transplantable cancers and augmented responses to checkpoint blockade immunotherapies. Similarly, in humans, vitamin D-induced genes correlate with improved responses to immune checkpoint inhibitor treatment as well as with immunity to cancer and increased overall survival. In mice, resistance is attributable to the activity of vitamin D on intestinal epithelial cells, which alters microbiome composition in favor of Bacteroides fragilis, which positively regulates cancer immunity. Our findings indicate a previously unappreciated connection between vitamin D, microbial commensal communities, and immune responses to cancer. Collectively, they highlight vitamin D levels as a potential determinant of cancer immunity and immunotherapy success.

Bigger babies: what happens in real practice in a non-academic UK center? Detection accuracy and outcomes with induction.

BACKGROUND: There is emerging evidence of improved outcomes with induction of labour for pregnancies in which the baby is thought to be large. This trial identifies scan accuracy and the effect of intervention for pregnancies complicated by suspected large for gestational age (LGA) on customized chart outside an academic center. METHODS: This is a retrospective cohort study of 3 groups of induced pregnancies; women with a suspected LGA fetus, women with diabetes (DM) and a control group (C) of women that underwent induction of labour on or after 280 days gestation. Data collection and analysis were prespecified. Scan accuracy and outcomes between the cohorts were compared. RESULTS: Over 1 year there were 845 cases: LGA (128), DM (116) and control cases (601). Mean birthweights differed significantly. PPV of EFW for birthweight >90th centile on GROW chart, WHO chart, and >4 kg was 0.35-0.40. Projected birthweight of >4 kg significantly better predicted itself (AUROC 0.70, 0.74 and 0.80). Mean scan error was -5.2% and +15.6% for DM and LGA. Shoulder dystocia and neonatal morbidity were not increased in LGA despite the significant increase in AVD 28/128, 21.9% vs. 99/601, 16.5%, aOR 2.20 (1.07-4.5). SVD was significantly less likely LGA vs. C at 69/128, 53.9% vs. 413/601, 68.7% aOR 0.38 (95% CI: 0.21-0.70). CONCLUSIONS: Third trimester EFW for bigger babies was poorly predictive of macrosomia. Fetal outcomes were good but women selected and induced as LGA had higher rates of hemorrhage and intervention.

The ballistic hip thrust test: a potential tool to monitor neuromuscular performance.

To investigate the reliability of the ballistic hip thrust (BHT) test performed on force plates as a diagnostic tool to monitor posterior chain neuromuscular status and compare its usefulness with the counter movement jump (CMJ). Twenty-two male football players from an elite Under-19 French Ligue 1 football club (16.4 ± 0.6 years) performed two assessments; Assessment 1: Two testing sessions separated by one week were performed to assess the reliability of the test. Participants performed a 3-set workout of 4 repetitions of the BHTs each session. Intra-day (between set) and inter-day (between testing day) reliability of concentric mean force, takeoff peak force and peak power were assessed. Assessment 2: Participants performed a 1-set workout of 3 repetitions of the CMJ and 4 repetitions of the BHT tests pre- and post-training to compare the usefulness of both tests. Concentric mean force and takeoff peak force showed small-to-moderate standardised typical errors (TE: 0.2-0.7) for inter- and intra-day reliability. Inter-day concentric mean force showed a coefficient of variation (CV) of 7.2%. Intra-day concentric mean force and take-off peak force showed a CV of 4.2% and 5.5%. BHT and CMJ showed similar moderate levels of usefulness. The BHT test showed moderate levels of reliability and usefulness. This test could be a useful addition to a testing battery to monitor posterior chain neuromuscular status.

Effect of turbulence, dispersion, and stratification on Escherichia coli disinfection in a subtropical maturation pond.

Sunlight disinfection is important for treatment of wastewater within maturation ponds. This study analyses the movement of Escherichi coli within a slice of a maturation pond, being affected by stratification, sunlight attenuation and mixing driven by wind shear and natural convection using computational fluid dynamics (CFD). Since the exposure to ultraviolet light is most effective in the near-surface region of the pond, natural convective mixing mechanisms to transport the pathogens from the lower parts of the pond are critical for disinfection efficacy. Different turbulence models are considered for closure of the momentum conservation equations and compared with a laminar flow simulation and a completely stirred tank reactor (CSTR) model. The effect of turbulence and stratification is shown to be significant for thermal and velocity distributions, and predictions of E. coli die-off. Greater volume-averaged E. coli die-off was predicted by the computationally convenient CSTR model than the CFD turbulence and laminar models. The simulation results are compared with experimental data and show that complete vertical mixing occurs in a diurnal pattern aiding die-off in sunlight-attenuating water. Practical applications of the model can assist in management strategies for maturation ponds such as off-take locations/times and evaluating seasonal variations in sunlight disinfection.

Occurrences of near-to-maximal speed-running bouts in elite soccer: insights for training prescription and injury mitigation.

Aim: To quantify the occurrence of near-to-maximal speed-running bouts in elite soccer players.Methods: Tracking match data from 35 professional players (23 ± 3 yrs) were analysed over four seasons (2015-2019). The number of runs reaching >80, >85 and >90% of players' individual maximal sprinting speed (MSS) was computed. Differences in peak speed occurrences between halves and positions were analysed.Results: Occurrences were not different between halves, but slightly-to-moderately position-dependent, with wingers (3.5 ± 2.0 runs >80% per half) and central midfielders (1.6 ± 1.5 runs >80% per half) performing the most and the least number of near-to-maximal speed-running bouts. Players did not reach >90% of MSS at all in 35% (Attackers) to 65% (Midfielders) of their matches; they reached ≥3 times >90% MSS per match in 2% (Midfielders) to 11% (Attackers) of their matches only. The maximal number of cumulated match occurrences during congested periods was observed in acentral defender (2 runs >90% of MSS every 4 days for 31 days) and a winger (1 run every 4 days for 52 days).Conclusions: The occurrence of near-to-maximal speed-running bouts is low in elite soccer. Match context, playing position and individual player profile are the main determinants of those occurrences.

Monitoring Cardiorespiratory Fitness in Professional Soccer Players: Is It Worth the Prick?

PURPOSE: To compare between-tests changes in submaximal exercise heart rate (HRex, 3 min, 12 km/h) and the speed associated with 4 mmol/L of blood lactate (V4mmol) in soccer players to get insight into their level of agreement and respective sensitivity to changes in players' fitness. METHODS: A total of 19 elite professional players (23 [3] y) performed 2 to 3 graded incremental treadmill tests (3-min stages interspersed with 1 min of passive recovery, starting speed 8 km/h, increment 2 km/h until exhaustion or 18 km/h if exhaustion was not reached before) over 1.5 seasons. The correlation between the changes in HRex and V4mmol was examined. Individual changes in the 2 variables were compared (>2 × typical error considered "clear"). RESULTS: The changes in HRex and V4mmol were largely correlated (r = .82; 90% confidence interval, .65-.91). In more than 90% of the cases, when a clear individual change in HRex was observed, it was associated with a similar clear change in V4mmol (the same direction, improvement, or impairment of fitness) and conversely. CONCLUSIONS: When it comes to testing players submaximally, the present results suggest that practitioners can use HRex or V4mmol interchangeably with confidence. However, in comparison with a field-based standardized warm-up run (3-4 min, all players together), the value of a multistage incremental test with repeated blood lactate samplings is questionable for a monitoring purpose given its time, labor, cost, and poorer player buy-in.

Hamstring Eccentric Strengthening Program: Does Training Volume Matter?

AIM: To compare the effect of low versus high volume of eccentric-biased hamstring training programs on knee-flexor strength and fascicle length changes in elite soccer players. METHODS: A total of 19 elite youth soccer players took part in this study and were randomly assigned into 2 subgroups. For 6 weeks in-season, the groups performed either a low-volume (1 set per exercise; 10 repetitions in total) or a high-volume (4 sets; 40 repetitions) eccentric training of their knee flexors. After 6-weeks midtraining (MID), players performed the alternate training regimen. Each training set consisted of 4 repetitions of the Nordic hamstring exercise and 6 repetitions of the bilateral stiff-leg deadlift. Eccentric knee-flexor strength (NordBord) as well as biceps femoris long head and semimembranosus fascicle length (scanned with ultrasound scanner) were assessed during pretraining (PRE), MID, and posttraining (POST) tests. RESULTS: Knee-flexor eccentric strength very likely increased from PRE to MID (low volume: +11.3% [7.8%] and high volume: 11.4% [5.3%]), with a possibly-to-likely increase in biceps femoris long head (+4.5% [5.0%] and 4.8% [2.5%]) and semimembranosus (+4.3% [4.7%] and 6.3% [6.3%]) fascicle length in both groups. There was no substantial changes between MID and POST. Overall, there was no clear between-group difference in the changes from PRE to MID and MID to POST for neither knee-flexor eccentric strength, biceps femoris long head, nor semimembranosus fascicle length. CONCLUSIONS: Low-volume knee-flexor eccentric training is as effective as a greater training dose to substantially improve knee-flexor strength and fascicle length in-season in young elite soccer players. Low volume is, however, likely more appropriate to be used in an elite team facing congested schedules.

Monitoring of Post-match Fatigue in Professional Soccer: Welcome to the Real World.

Participation in soccer match-play leads to acute and transient subjective, biochemical, metabolic and physical disturbances in players over subsequent hours and days. Inadequate time for rest and regeneration between matches can expose players to the risk of training and competing whilst not entirely recovered. In professional soccer, contemporary competitive schedules can require teams to compete in excess of 60 matches over the course of the season with periods of fixture congestion occurring, prompting much attention from researchers and practitioners to the monitoring of fatigue and readiness to play. A comprehensive body of research has investigated post-match acute and residual fatigue responses. Yet the relevance of the research for professional soccer contexts is debatable, notably in relation to the study populations and designs employed. Monitoring can indeed be invasive, expensive, time inefficient, and difficult to perform routinely and simultaneously in a large squad of regularly competing players. Uncertainty also exists regarding the meaningfulness and interpretation of changes in fatigue response values and their functional relevance, and practical applicability in the field. The real-world need and cost-benefit of monitoring must be carefully weighed up. In relation to professional soccer contexts, this opinion paper intends to (1) debate the need for post-match fatigue monitoring; (2) critique the real-world relevance of the current research literature; (3) discuss the practical burden relating to measurement tools and protocols, and the collection, interpretation and application of data in the field; and (4) propose future research perspectives.

Monitoring Players' Readiness Using Predicted Heart-Rate Responses to Soccer Drills.

PURPOSE: To examine the ability of multivariate models to predict the heart-rate (HR) responses to some specific training drills from various global positioning system (GPS) variables and to examine the usefulness of the difference in predicted vs actual HR responses as an index of fitness or readiness to perform. METHOD: All data were collected during 1 season (2016-17) with players' soccer activity recorded using 5-Hz GPS and internal load monitored using HR. GPS and HR data were analyzed during typical small-sided games and a 4-min standardized submaximal run (12 km·h-1). A multiple stepwise regression analysis was used to identify which combinations of GPS variables showed the largest correlations with HR responses at the individual level (HRACT, 149 [46] GPS/HR pairs per player) and was further used to predict HR during individual drills (HRPRED). Then, HR predicted was compared with actual HR to compute an index of fitness or readiness to perform (HRΔ, %). The validity of HRΔ was examined while comparing changes in HRΔ with the changes in HR responses to a submaximal run (HRRUN, fitness criterion) and as a function of the different phases of the season (with fitness being expected to increase after the preseason). RESULTS: HRPRED was very largely correlated with HRACT (r = .78 [.04]). Within-player changes in HRΔ were largely correlated with within-player changes in HRRUN (r = .66, .50-.82). HRΔ very likely decreased from July (3.1% [2.0%]) to August (0.8% [2.2%]) and most likely decreased further in September (-1.5% [2.1%]). CONCLUSIONS: HRΔ is a valid variable to monitor elite soccer players' fitness and allows fitness monitoring on a daily basis during normal practice, decreasing the need for formal testing.

Neuromuscular Responses to Conditioned Soccer Sessions Assessed via GPS-Embedded Accelerometers: Insights Into Tactical Periodization.

PURPOSE: To examine the reliability of field-based running-specific measures of neuromuscular function assessed using global positioning system (GPS)-embedded accelerometers and their responses to 3 typical conditioned sessions (ie, strength, endurance, and speed) in elite soccer players. METHODS: Before and immediately after each session, vertical jump (countermovement jump [CMJ]) and adductor squeeze strength (groin) performances were recorded. Players also performed a 4-min run at 12 km/h followed by four ∼60-m runs (run = 12 s, r = 33 s). GPS (5 Hz) and accelerometer (100 Hz) data collected during the 4 runs and the recovery periods, excluding the last recovery period, were used to derive vertical stiffness (K), peak loading force (peak force over all the foot strikes [Fpeak]), and propulsion efficiency (ie, the ratio between velocity and force loads [Vl/Fl]). RESULTS: Typical errors were small (CMJ, groin, K, and Vl/Fl) and moderate (Fpeak), with moderate (Fpeak), high (K and Vl/Fl), and very high ICCs (CMJ and groin). After all sessions, there were small decreases in groin and increases in K, but changes in F were all unclear. By contrast, the CMJ and Vl/Fl ratio responses were session dependent. There was a small increase in CMJ after speed and endurance, but unclear changes after strength; the Vl/Fl ratio increased substantially after strength, but there were a small and a moderate decrease after endurance and speed, respectively. CONCLUSIONS: Running-specific measures of neuromuscular function assessed in the field via GPS-embedded accelerometers show acceptable levels of reliability. Although the 3 sessions examined may be associated with limited neuromuscular fatigue, changes in neuromuscular performance and propulsion efficiency are likely session-objective dependent.

Small-Sided Games in Elite Soccer: Does One Size Fit All?

PURPOSE: To compare the peak intensity of typical small-sided games (SSGs) with those of official matches in terms of running demands and mechanical work (MechW) over different rolling average durations and playing positions. METHODS: Data were collected in 21 players (25 [5] y, 181 [7] cm, and 77 [7] kg) belonging to an elite French football team. SSG data were collected over 2 seasons during typical training sessions (249 files, 12 [4] per player) and official matches (n = 12). Players' locomotor activity was recorded using 5-Hz Global Positioning System. Total distance (m), high-speed distance (HS, distance above 14.4 km·h-1, m), and MechW (a.u.) were analyzed during different rolling average periods (1-15 min). The SSGs examined were 4v4+goalkeepers (GKs), 6v6+GKs, 8v8+GKs, and 10v10+GKs. RESULTS: Peak total distance and HS during 4v4, 6v6, and 8v8 were likely-to-most likely lower than during matches (effect size: -0.59 [±0.38] to -7.36 [±1.20]). MechW during 4v4 was likely-to-most likely higher than during matches (1-4 min; 0.61 [±0.77] to 2.30 [±0.64]). Relative to their match demands, central defenders performed more HS than other positions (0.63 [±0.81] to 1.61 [±0.52]) during 6v6. Similarly, central midfielders performed less MechW than the other positions during 6v6 (0.68 [±0.72] to 1.34 [±0.99]) and 8v8 (0.73 [±0.50] to 1.39 [±0.32]). CONCLUSION: Peak locomotor intensity can be modulated during SSGs of various formats and durations to either overload or underload match demands, with 4v4 placing the greatest and the least emphasis on MechW and HS, respectively. Additionally, in relation to match demands central defenders and central midfielders tend to be the most and least overloaded during SSGs, respectively.

Locomotor and Heart Rate Responses of Floaters During Small-Sided Games in Elite Soccer Players: Effect of Pitch Size and Inclusion of Goalkeepers.

PURPOSE: To (1) compare the locomotor and heart rate responses between floaters and regular players during both small and large small-sided games (SSGs) and (2) examine whether the type of game (ie, game simulation [GS] vs possession game [PO]) affects the magnitude of the difference between floaters and regular players. METHODS: Data were collected in 41 players belonging to an elite French football team during 3 consecutive seasons (2014-2017). A 5-Hz global positionning system was used to collect all training data, with the Athletic Data Innovation analyzer (v5.4.1.514) used to derive total distance (m), high-speed distance (>14.4 km·h-1, m), and external mechanical load (MechL, a.u.). All SSGs included exclusively 1 floater and were divided into 2 main categories, according to the participation of goalkeepers (GS) or not (PO) and then further divided into small and large (>100 m2per player) SSGs based on the area per player ratio. RESULTS: Locomotor activity and MechL performed were likely-to-most likely lower (moderate to large magnitude) in floaters compared with regular players, whereas differences in heart rate responses were unclear to possibly higher (small) in floaters. The magnitude of the difference in locomotor activity and MechL between floaters and regular players was substantially greater during GS compared with PO. CONCLUSIONS: Compared with regular players, floaters present decreased external load (both locomotor and MechL) despite unclear to possibly slightly higher heart rate responses during SSGs. Moreover, the responses of floaters compared with regular players are not consistent across different sizes of SSGs, with greater differences during GS than PO.

Player-Tracking Technology: Half-Full or Half-Empty Glass?

With the ongoing development of microtechnology, player tracking has become one of the most important components of load monitoring in team sports. The 3 main objectives of player tracking are better understanding of practice (provide an objective, a posteriori evaluation of external load and locomotor demands of any given session or match), optimization of training-load patterns at the team level, and decision making on individual players' training programs to improve performance and prevent injuries (eg, top-up training vs unloading sequences, return to play progression). This paper discusses the basics of a simple tracking approach and the need to integrate multiple systems. The limitations of some of the most used variables in the field (including metabolic-power measures) are debated, and innovative and potentially new powerful variables are presented. The foundations of a successful player-monitoring system are probably laid on the pitch first, in the way practitioners collect their own tracking data, given the limitations of each variable, and how they report and use all this information, rather than in the technology and the variables per se. Overall, the decision to use any tracking technology or new variable should always be considered with a cost/benefit approach (ie, cost, ease of use, portability, manpower/ability to affect the training program).

Relative Match Intensities at High Altitude in Highly-Trained Young Soccer Players (ISA3600).

To compare relative match intensities of sea-level versus high-altitude native soccer players during a 2-week camp at 3600 m, data from 7 sea-level (Australian U17 National team, AUS) and 6 high-altitude (a Bolivian U18 team, BOL) native soccer players were analysed. Two matches were played at sea-level and three at 3600 m on Days 1, 6 and 13. The Yo-Yo Intermittent recovery test (vYo-YoIR1) was performed at sea-level, and on Days 3 and 10. Match activity profiles were measured via 10-Hz GPS. Distance covered >14.4 km.h(-1) (D>14.4 km·h(-1)) and >80% of vYo-YoIR1 (D>80%vYo-YoIR1) were examined. Upon arrival at altitude, there was a greater decrement in vYo-YoIR1 (Cohen's d +1.0, 90%CL ± 0.8) and D>14.4 km·h(-1) (+0.5 ± 0.8) in AUS. D>14.4 km.h(-1) was similarly reduced relative to vYo-YoIR1 in both groups, so that D>80%vYo-YoIR1 remained similarly unchanged (-0.1 ± 0.8). Throughout the altitude sojourn, vYo-YoIR1 and D>14.4 km·h(-1) increased in parallel in AUS, so that D>80%vYo-YoIR1 remained stable in AUS (+6.0%/match, 90%CL ± 6.7); conversely D>80%vYo-YoIR1 decreased largely in BOL (-12.2%/match ± 6.2). In sea-level natives competing at high-altitude, changes in match running performance likely follow those in high-intensity running performance. Bolivian data confirm that increases in 'fitness' do not necessarily translate into greater match running performance, but rather in reduced relative exercise intensity. Key pointsWhen playing at high-altitude, players may alter their activities during matches in relation to their transient maximal physical capacities, possibly to maintain a 'tolerable' relative exercise intensity.While there is no doubt that running performance per se in not the main determinant of match outcomes (Carling, 2013), fitness levels influence relative match intensity (Buchheit et al., 2012, Mendez-Villanueva et al., 2013), which in-turn may impact on decision making and skill performance (Rampinini et al., 2008).In the context of high-altitude competitions, it is therefore recommended to arrive early enough (i.e., ~2 weeks) to allow (at least partial) acclimatisation, and in turn, allow sea-level native players to regulate their running activities in relation to both actual game demands and relative match intensity.

Peak match speed and maximal sprinting speed in young soccer players: effect of age and playing position.

This study assessed the relationship between peak match speed (PMS) and maximal sprinting speed (MSS) in regard to age and playing positions. MSS and absolute PMS (PMSAbs) were collected from 180 male youth soccer players (U13-U17, 15.0 ± 1.2 y, 161.5 ± 9.2 cm, and 48.3 ± 8.7 kg). The fastest 10-m split over a 40-m sprint was used to determine MSS. PMSAbs was recorded using a global positioning system and was also expressed as a percentage of MSS (PMSRel). Sprint data were compared between age groups and between playing positions. Results showed that regardless of age and playing positions, faster players were likely to reach higher PMSAbs and possibly lower PMSRel. Despite a lower PMSAbs than in older groups (eg, 23.4 ± 1.8 vs 26.8 ± 1.9 km/h for U13 and U17, respectively, ES = 1.9 90%, confidence limits [1.6;2.1]), younger players reached a greater PMSRel (92.0% ± 6.3% vs. 87.2% ± 5.7% for U13 and U17, respectively, ES = -0.8 90% CL [-1.0;-0.5]). Playing position also affected PMSAbs and PMSRel, as strikers were likely to reach higher PMSAbs (eg, 27.0 ± 2.7 vs 23.6 ± 2.2 km/h for strikers and central midfielders, respectively, ES = 2.0 [1.7;2.2]) and PMSRel (eg, 93.6% ± 5.2% vs 85.3% ± 6.5% for strikers and central midfielders, respectively, ES = 1.0 [0.7;1.3]) than all other positions. The findings confirm that age and playing position affect the absolute and relative intensity of speed-related actions during matches.

Monitoring changes in jump and sprint performance: best or average values?

This study compares different approaches to monitor changes in jump and sprint performance while using either the best or the average performance of repeated trials. One hundred two highly trained young footballers (U13 to U17) performed, in 2 different testing sessions separated by 4 mo, 3 countermovement jumps (n = 87) and 2 sprints (n = 98) over 40 m with 10-m splits to assess acceleration (first 10 m) and maximal sprinting speed (best split, MSS). Standardized group-average changes between the 2 testing periods and the typical error (TE) were calculated and compared for each method. The likelihood of substantial changes in performance for each individual player was also calculated. There was a small increase in jump performance (+6.1% for best and +7% for average performance). While 10-m time was likely unchanged (+~1.2% for both best and average performance), MSS showed likely small improvements (+~2.0% for both best and average performance). The TEs for jumping performance were 4.8% (90% confidence limits 4.3;5.6) and 4.3% (3.8;5.0) for best and average values, respectively; 1.8% (1.6;2.1) and 1.7% (1.5;1.9) for 10-m time and 2.0% (1.8;2.3) and 2.0% (1.8;2.3) for MSS. The standardized differences between TE were likely unclear or trivial for all comparisons (eg, 10-m, 0.01 [-0.09;0.10]). The numbers of players showing a likely increase or decrease in performance were 30/0 and 29/0 for best and average jump performances, 9/4 and 12/2 for 10-m times, and 33/4 and 33/4 for MSS. In conclusion, the 2 monitoring approaches are likely to provide similar outcomes.

Monitoring accelerations with GPS in football: time to slow down?

The aims of the current study were to examine the magnitude of between-GPS-models differences in commonly reported running-based measures in football, examine between-units variability, and assess the effect of software updates on these measures. Fifty identical-brand GPS units (15 SPI-proX and 35 SPIproX2, 15 Hz, GPSports, Canberra, Australia) were attached to a custom-made plastic sled towed by a player performing simulated match running activities. GPS data collected during training sessions over 4 wk from 4 professional football players (N = 53 files) were also analyzed before and after 2 manufacturer-supplied software updates. There were substantial differences between the different models (eg, standardized difference for the number of acceleration >4 m/s2 = 2.1; 90% confidence limits [1.4, 2.7], with 100% chance of a true difference). Between-units variations ranged from 1% (maximal speed) to 56% (number of deceleration >4 m/s2). Some GPS units measured 2-6 times more acceleration/deceleration occurrences than others. Software updates did not substantially affect the distance covered at different speeds or peak speed reached, but 1 of the updates led to large and small decreases in the occurrence of accelerations (-1.24; -1.32, -1.15) and decelerations (-0.45; -0.48, -0.41), respectively. Practitioners are advised to apply care when comparing data collected with different models or units or when updating their software. The metrics of accelerations and decelerations show the most variability in GPS monitoring and must be interpreted cautiously.