Iron Nanoparticle-Incorporated Laser-Induced Graphene Filters for Environmental Remediation via an In Situ Electro-Fenton Process.

Laser-induced graphene (LIG) has garnered much attention due to its facile and chemically free fabrication technique. Metal nanoparticle incorporation into the LIG matrix can improve its electrical and catalytical properties for environmental application. Here, we demonstrate the fabrication of nanoscale zerovalent iron (nZVI) nanoparticle-incorporated LIG (Fe-LIG) and sulfidized-nanoscale zerovalent iron (S-nZVI) nanoparticle-incorporated LIG (SFe-LIG) surfaces. The sheets were first fabricated to investigate nanoparticle loading, successful incorporation in the LIG matrix, and electrochemical performance as electrodes. Fe-LIG and SFe-LIG sheets showed ∼3-3.5 times more charge density as compared with the control LIG sheet. The XPS and its deconvolution confirmed the presence of nZVI and S-nZVI in the Fe-LIG and SFe-LIG surfaces, which can generate in situ hydroxyl radical (•OH) via iron activation of electrogenerated hydrogen peroxide (H2O2) in short in situ electro-Fenton process. After confirmation of the successful incorporation of iron-based nanoparticles in the LIG matrix, filters were fabricated to demonstrate the application in the flow-through filtration. The Fe-LIG and SFe-LIG filters showed ∼10-30% enhanced methylene blue removal under the application of 2.5 V at ∼1000 LMH flux. The Fe-LIG and SFe-LIG filters also showed complete 6-log bacteria and virus removal at 2.5 and 5 V, respectively, while the LIG filters showed only ∼4-log removal. Such enhanced removal by the Fe-LIG and SFe-LIG filters as compared to LIG filters is attributed to the improved charge density, electrochemical activity, and in situ electro-Fenton process. The study shows the potential to develop catalytic LIG-based surfaces for various applications, including contaminant removal and microbial inactivation.

Solar interfacial evaporation devices for desalination and water treatment: Perspective and future.

Water is an essential commodity for society, and alternate resources such as seawater and wastewater are vital for the future. There are various desalination technologies that can provide sufficient and sustainable water sources. Renewable energy-based desalination technologies like solar-based interfacial evaporation are very efficient and sustainable desalination methods. Solar-based interfacial evaporation has been a focus due to its efficient and easy-to-use methods. Still, research is needed for fouling resistance, scalable and low-cost materials, and devices for solar interfacial evaporation. Recent research focuses on the materials for evaporation devices, but various other aspects of device design and fabrication methods are also necessary to improve device performance. In this article, all the evaporator device configurations and strategies for efficient evaporator devices are compiled and summarized. The evaporator devices have been classified into eight main categories: monolayer, bilayer, tree-like design, low-temperature designs, 3D-Origami-based designs, latent heat recovery design, design with storage/batch process, and contactless design. It was found that a good absorber, well-engineered air-water interface, and bottom-layer insulation are necessary for the best systems. The current research focuses on the vapor production output of the devices but not on the water production from devices. So, the focus on device-based water production and the associated cost of the water produced is essential. This article articulates the strategies and various scalable and efficient devices for evaporation-based solar-driven desalination. This article will be helpful for the researchers in improving devices output and coming up with a sustainable desalination and water treatment.

Performance evaluation of sulfidated nanoscale iron for hexavalent chromium removal from groundwater in sequential batch study.

Chromium [Cr] contamination in groundwater is one of the serious environmental concerns due to the carcinogenicity of its water-soluble and mobile hexavalent [Cr(VI)] form. In spite of the existence of multiple precipitation and adsorption-based Cr(VI) remediation technologies, the usage of sulfidated nano zerovalent iron (S-nZVI) has recently attracted researchers due to its high selectivity. Although S-nZVI effectively immobilized Cr(VI), its long-term performance in multiple shifted equilibrium has not been explored. In this contribution, influences of S-nZVI dosage, initial concentration of Cr(VI), pH, ionic strength, total hardness, sulfate, carbonate, and silicate were probed in ultrapure water. Further experiments were performed in synthetic groundwater to investigate the effects of initial concentration of Cr(VI) in the pH range of 4-8 for 1 g L-1 S-nZVI dosage. Cr(VI) removal rate was quantified in groundwater without pH fixation. Finally, a comparative study between conventional nano zerovalent iron (nZVI) and S-nZVI was conducted in sequential batch reactors to investigate their respective efficiencies during repeated usage. Mechanistic interpretation of the processes governing the immobilization of Cr(VI) was done by integrating the results of these experiments with the metadata. While aggregation due to magnetic properties and rapid oxidation of Fe decreased the efficiency of nZVI with repeated usage, sulfidation minimized the passivation and favored an extended reducing environment because of continuous electron transfer from iron and sulfur components.

Role of organisms and their enzymes in the biodegradation of microplastics and nanoplastics: A review.

Microplastic (MP) and Nanoplastic (NP) contamination have become a critical ecological concern due to their persistent presence in every aspect of the ecosystem and their potentially harmful effects. The current approaches to eradicate these wastes by burning up and dumping adversely impact the environment, while recycling has its own challenges. As a result, applying degradation techniques to eliminate these recalcitrant polymers has been a focus of scientific investigation in the recent past. Biological, photocatalytic, electrocatalytic, and, recently, nanotechnologies have been studied to degrade these polymers. Nevertheless, it is hard to degrade MPs and NPs in the environment, and these degradation techniques are comparatively inefficient and require further development. The recent research focuses on the potential use of microbes to degrade MPs and NPs as a sustainable solution. Therefore, considering the recent advancements in this important research field, this review highlights the utilization of organisms and enzymes for the biodegradation of the MPs and NPs with their probable degradation mechanisms. This review provides insight into various microbial entities and their enzymes for the biodegradation of MPs. In addition, owing to the lack of research on the biodegradation of NPs, the perspective of applying these processes to NPs degradation has also been looked at. Finally, a critical evaluation of the recent development and perspective for future research to improve the effective removal of MPs and NPs in the environment through biodegradation is also discussed.

In-situ fabrication of titanium suboxide-laser induced graphene composites: Removal of organic pollutants and MS2 Bacteriophage.

Titanium suboxides (TSO) are identified as a series of compounds showing excellent electro- and photo-chemical properties. TSO composites with carbon-based materials such as graphene have further improved water splitting and pollutant removal performance. However, their expensive and multi-step synthesis limits their wide-scale use. Furthermore, recently discovered laser-induced graphene (LIG) is a single-step and low-cost fabrication of graphene-based composites. Moreover, LIG's highly electrically conductive surface aids in tremendous environmental applications, including bacterial inactivation, anti-biofouling, and pollutant sensing. Here, we demonstrate the single-step in-situ fabrication of TSO-LIG composite by directly scribing the TiO2 mixed poly(ether) sulfone sheets using a CO2 infrared laser. In contrast, earlier composites were derived from either commercial-grade TSO or synthesized TSO with graphene in multi step processes. The characteristic Ti3+ peaks in XPS confirmed the conversion of TiO2 into its sub-stoichiometric form, enhancing the electro-catalytical properties of the LIG-TiOx composite surface. Electrochemical characterization, including impedance spectroscopy, validated the surface's enhanced electrochemical activity and electrode stability. Furthermore, the LIG-TiOx composite surfaces were tested for anti-biofouling action and electrochemical application as electrodes and filters. The composite electrodes exhibit enhanced degradation performance for removing emerging pollutant antibiotics ciprofloxacin and methylene blue due to the in-situ hydroxyl radical generation. Additionally, the LIG-TiOx conductive filters showed the complete 6-log killing of mixed bacterial culture and MS2 phage virus in flow-through filtration mode at 2.5 V, which is ∼2.5-log more killing compared to non-composited LIG filers at 500 Lm-2h-1. Nevertheless, these cost-effective LIG-TiOx composites have excellent electrical properties and can be effectively utilized for energy and environmental applications.

Effect of Laser Parameters on Laser-Induced Graphene Filter Fabrication and Its Performance for Desalination and Water Purification.

Laser-induced graphene (LIG) is a low-cost, chemical-free single-step fabrication process and has shown its potential in water treatment, electronics, and sensing. LIG fabrication optimization is mostly explored for dense polyimide (PI) polymers. However, LIG-based filters and membranes for water treatment need to be porous, and additional steps are required to get porous surfaces from PI-based surfaces. Polyethersulfone (PES) porous membranes are cost-effective and are common in water purification as compared to PI; further, the optimization of LIG fabrication on PES-based porous membranes is not explored. So, this study demonstrated the fabrication, optimization, and characterization of LIG with different laser parameters such as power, speed, image density (ID), focusing, laser platforms, and membrane support layer effect on porous PES commercial (UP010) and lab-casted 15% PES (PES15) membranes. The performance of optimized LIG filters was tested for interfacial evaporation (IE)-based desalination in single and stacked layer configuration and water purification applications such as dye removal and disinfection. IE was done in Joule heating (JH) and solar heating (SH) modes, and the UP010-ID7 LIG filter showed the highest JH evaporation rates of ∼1.1, 1.8, and 2.82 kg m-2 h-1 in single, double, and triple stacked configurations, respectively. Using a JH IE setup, the best-performing UP010-ID7 LIG filters have also shown ∼100% removal of methylene blue dye from the contaminated water. Furthermore, all LIG filters showed a complete 6-log bacterial inhibition at the 5 V filtration experiments; at 2.5 V, the optimized LIG filters showed a higher removal than the non-optimized filters. Additionally, the LIGs obtained with the aluminum platform were the best quality. This work demonstrates that laser power, ID, platform, and membrane support are critical parameters for the best-performing PES-LIG filters, and they can be effectively utilized to fabricate PES-based LIG porous surfaces for various energy, environmental, and catalysis applications.

Design, synthesis, and application of thermally responsive draw solutes for sustainable forward osmosis desalination: A review.

Forward osmosis (FO) is an emerging sustainable desalination technology; however, it is not a stand-alone process and requires an additional step to recover the water or regenerate the draw solute (DS), making it energy extensive. Therefore, incorporating inexpensive energy sources for DS regeneration is a viable solution to compete with reverse osmosis desalination technology. Hence, selecting suitable DS and its regeneration became a crucial research focus in FO desalination. Among various DSs reported, thermally responsive DSs (TRDS) provide an opportunity to integrate low-grade energy sources for DS regeneration. Utilizing such inexpensive energy will reduce fossil fuel energy demand, lower the cost of desalination, and minimize the carbon footprint. Hence, this review explores the TRDS for FO-based desalination with its design, synthesis, and applications. The manuscript has discussed the classification and selection criteria for the DSs, and how traditional and new-generation TRDSs are designed and synthesized from cationic and anionic moieties of ionic liquids, hydrogels, and other chemicals. The manuscript has also given importance to design criteria such as osmotic strength, viscosity, toxicity, and thermal stability for TRDSs. Furthermore, a detailed discussion on the FO performance, energy, and economic aspects of TRDSs has been reviewed, along with a discussion on the possible low-grade energy sources for the recovery of TRDS. Finally, the challenges and future directions for TRDSs have been discussed to drive FO toward sustainable desalination technology.

Laser-induced graphene-based electrochemical biosensors for environmental applications: a perspective.

Biosensors are miniaturized devices that provide the advantage of in situ and point-of-care monitoring of analytes of interest. Electrochemical biosensors use the mechanism of oxidation-reduction reactions and measurement of corresponding electron transfer as changes in current, voltage, or other parameters using different electrochemical techniques. The use of electrochemically active materials is critical for the effective functioning of electrochemical biosensors. Laser-induced graphene (LIG) has garnered increasing interest in biosensor development and improvement due to its high electrical conductivity, specific surface area, and simple and scalable fabrication process. The effort of this perspective is to understand the existing classes of analytes and the mechanisms of their detection using LIG-based biosensors. The manuscript has highlighted the potential use of LIG, its modifications, and its use with various receptors for sensing various environmental pollutants. Although the conventional graphene-based sensors effectively detect trace levels for many analytes in different applications, the chemical and energy-intensive fabrication and time-consuming processes make it imperative to explore a low-cost and scalable option such as LIG for biosensors production. The focus of these potential biosensors has been kept on detection analytes of environmental significance such as heavy metals ions, organic and inorganic compounds, fertilizers, pesticides, pathogens, and antibiotics. The use of LIG directly as an electrode, its modifications with nanomaterials and polymers, and its combination with bioreceptors such as aptamers and polymers has been summarized. The strengths, weaknesses, opportunities, and threats analysis has also been done to understand the viability of incorporating LIG-based electrochemical biosensors for environmental applications.

Magnéli-Phase Ti4O7-Doped Laser-Induced Graphene Surfaces and Filters for Pollutant Degradation and Microorganism Removal.

Laser-induced graphene (LIG) has recently become a point of attraction globally as an environmentally friendly method to fabricate graphene foam in a single step using a CO2 laser. The electrical properties of LIG are studied in different environmental applications, such as bacterial inactivation, antibiofouling, and pollutant sensing. Furthermore, metal or nonmetal doping of graphene enhances its catalytical performance in pollutant degradation and decontamination. Magnéli phase (TinO2n-1) is a substoichiometric titanium oxide known for its high electrocatalytic behavior and chemical inertness and is being explored as a membrane or electrode material for environmental decontamination. Here, we show the fabrication and characterization of LIG-Magnéli-phase (Ti4O7) titanium suboxide composites as electrodes and filters on poly(ether sulfone). Unlike undoped LIG electrodes, the doped Ti4O7-LIG electrodes exhibit enhanced electrochemical activity, as demonstrated in electrochemical characterization using cyclic voltammetry and electrochemical impedance spectroscopy. Due to the in situ generation of hydroxyl radicals on the surface, the doped electrodes exhibit increase in methylene blue degradation and microorganism removal. Effects of voltage and doping were examined, resulting in a clear trend of degradation and decontamination performance proportional to the doping concentration and applied voltage giving the best result at 2.5 V for 10% Ti4O7 doping. The LIG-Ti4O7 surfaces also showed biofilm inhibition against mixed bacterial culture. The flow-through filtration using a LIG-Ti4O7 conductive filter showed complete bacterial killing with 6 log removal in the permeate at 2.5 V, an enhancement of ∼2.5 log compared to undoped LIG filters at a flow rate of ∼500 L m-2 h-1. The facile fabrication of Ti4O7-doped LIG with enhanced electrochemical properties can be effectively used for energy and environmental applications.

Magnéli phase titanium sub-oxides synthesis, fabrication and its application for environmental remediation: Current status and prospect.

Sub-stoichiometric titanium oxide, also called titanium suboxides (TSO), had been a focus of research for many decades with a chemical composition of TinO2n-1 (n ≥ 1). It has a unique oxygen-deficient crystal structure which provides it an outstanding electrical conductivity and high corrosion resistance similar to ceramic materials. High electrical conductivity and ability to sustain in adverse media make these phases a point of attention for researchers in energy storage and environmental remediation applications. The Magnéli phase-based reactive electroconductive membranes (REM) and electrodes have demonstrated the electrochemical oxidation of pollutants in the water in flow-through and flow by configuration. Additionally, it has also shown its potential for visible light photochemical degradation as well. This review attempts to summarize state of the art in various Magnéli phases materials synthesis routes and their electrochemical and photochemical ability for environmental application. The manuscript introduces the Magnéli phase, its crystal structure, and catalytic properties, followed by the recent development in synthesis methods from diverse titanium sources, notably TiO2 through thermal reduction. The various fabrication methods for Magnéli phase-base REMs and electrodes have also been summarized. Furthermore, the article discussed the environmental remediations via electrochemical and photochemical advanced oxidation processes. Additionally, the hybrid technology with REMs and electrodes is used to counter membrane biofouling and develop electrochemical sensing devices for the pollutants. The Magnéli phase materials have a bright future for both electrochemical and photochemical advanced oxidation of emerging contaminants in water and wastewater treatment.

Laser-Induced Graphene (LIG) as a Smart and Sustainable Material to Restrain Pandemics and Endemics: A Perspective.

A healthy environment is necessary for a human being to survive. The contagious COVID-19 virus has disastrously contaminated the environment, leading to direct or indirect transmission. Therefore, the environment demands adequate prevention and control strategies at the beginning of the viral spread. Laser-induced graphene (LIG) is a three-dimensional carbon-based nanomaterial fabricated in a single step on a wide variety of low-cost to high-quality carbonaceous materials without using any additional chemicals potentially used for antiviral, antibacterial, and sensing applications. LIG has extraordinary properties, including high surface area, electrical and thermal conductivity, environmental-friendliness, easy fabrication, and patterning, making it a sustainable material for controlling SARS-CoV-2 or similar pandemic transmission through different sources. LIG's antiviral, antibacterial, and antibiofouling properties were mainly due to the thermal and electrical properties and texture derived from nanofibers and micropores. This perspective will highlight the conducted research and the future possibilities on LIG for its antimicrobial, antiviral, antibiofouling, and sensing applications. It will also manifest the idea of incorporating this sustainable material into different technologies like air purifiers, antiviral surfaces, wearable sensors, water filters, sludge treatment, and biosensing. It will pave a roadmap to explore this single-step fabrication technique of graphene to deal with pandemics and endemics in the coming future.

Biocatalytic membranes for combating the challenges of membrane fouling and micropollutants in water purification: A review.

Over the last few years, the list of water contaminants has grown tremendously due to many anthropogenic activities. Various conventional technologies are available for water and wastewater treatment. However, micropollutants of emerging concern (MEC) are posing a great threat due to their activity at trace concentration and poor removal efficiency by the conventional treatment processes. Advanced technology like membrane technology can remove MEC to some extent. However, issues like the different chemical properties of MEC, selectivity, and fouling of membranes can affect the removal efficiency. Moreover, the concentrate from the membrane filtration may need further treatment. Enzymatic degradation of pollutants and foulants is one of the green approaches for removing various contaminants from the water as well as mitigating membrane fouling. Biocatalytic membranes (BCMs), in which enzymes are immobilized on membranes, combines the advantages of membrane separation and enzymatic degradation. This review article discussed various commonly used enzymes in BCMs for removing MEC and fouling. The majorly used enzymes were oxidoreductases and hydrolases for removing MEC, antifouling, and self-cleaning ability. The various BCM synthesis processes based on entrapment, crosslinking, and binding have been summarized, along with the effects of the addition of the nanoparticles on the performances of the BCMs. The scale-up, commercial viability, challenges, and future direction for improving BCMs have been discussed and shown bright possibilities for these new generation membranes.

The Future of Flash Graphene for the Sustainable Management of Solid Waste.

Graphene research has steadily increased, and its commercialization in many applications is becoming a reality because of its superior physicochemical properties and advances in synthesis techniques. However, bulk-scale production of graphene still requires large amounts of solvents, electrochemical treatment, or sonication. Recently, a method was discovered to convert bulk quantities of carbonaceous materials to graphene using flash Joule heating (FJH) and, so named, flash graphene (FG). This method can be used to turn various solid wastes containing the prerequisite element carbon into FG. Globally, more than 2 billion tons of municipal solid waste (MSW) are generated every year and, in many municipalities, are becoming unmanageable. The most commonly used waste management methods include recycling, composting, anaerobic digestion, incineration, gasification, pyrolysis, and landfill disposal. However, around 70% of global waste ends up in landfills or open dumps, while the rest is recycled, composted, or incinerated. Even the various waste valorization techniques, such as pyrolysis and gasification, produce some waste residues that have their ultimate destination in landfills. Thus, technologies that can minimize waste volume or convert waste into valuable products are required. The thermal treatment process of FJH for FG production provides both waste volume reduction and valorization in the form of FG. In this Perspective, we provide an overview of FJH and its possible applications in various types of waste conversion/valorization. We describe the typical current MSW management system as well as the potential for creating FG at various stages and propose a schematic plan for the incorporation of FG in MSW management. We also analyze the strengths, weaknesses, opportunities, and threats of MSW as an FG precursor in terms of technical, economic, environmental, and social sustainability. This valuable waste valorization and management strategy can help achieve near-zero waste and an economy-boosting MSW management system.

Virus Inactivation in Water Using Laser-Induced Graphene Filters.

Interest in the pathogenesis, detection, and prevention of viral infections has increased broadly in many fields of research over the past year. The development of water treatment technology to combat viral infection by inactivation or disinfection might play a key role in infection prevention in places where drinking water sources are biologically contaminated. Laser-induced graphene (LIG) has antimicrobial and antifouling surface effects mainly because of its electrochemical properties and texture, and LIG-based water filters have been used for the inactivation of bacteria. However, the antiviral activity of LIG-based filters has not yet been explored. Here we show that LIG filters also have antiviral effects by applying electrical potential during filtration of the model prototypic poxvirus Vaccinia lister. This antiviral activity of the LIG filters was compared with its antibacterial activity, which showed that higher voltages were required for the inactivation of viruses compared to that of bacteria. The generation of reactive oxygen species, along with surface electrical effects, played a role in the mechanism of virus inactivation. This new property of LIG highlights its potential for use in water and wastewater treatment for the electrochemical disinfection of various pathogenic microorganisms, including bacteria and viruses.

Silver-doped laser-induced graphene for potent surface antibacterial activity and anti-biofilm action.

Previously, laser-induced graphene (LIG) coated surfaces were shown to resist biofilm growth, although the material was not strongly antibacterial. Here, we show LIG surfaces doped with silver nanoparticles (Ag0 or AgNPs) as highly antibacterial surfaces. Starting from AgNO3 polyethersulfone (PES) polymer substrates, silver nanoparticles between 5-10 nm were generated in situ during the lasing process and stably embedded in the fibrous and porous structure of LIG in a single step. These silver doped LIG (Ag@LIG) surfaces were highly toxic to bacteria via a mechanism of both Ag+ ion release and possible surface toxicity of the AgNPs. The added antibacterial function of Ag-nanoparticles expands the functionality of LIG coated surfaces and might lead to highly effective point of use/entry devices in rural areas or in disaster situations with contaminated water sources.

Laser-Induced Graphene-PVA Composites as Robust Electrically Conductive Water Treatment Membranes.

Graphene nanomaterials can feature both superb electrical conductivity and unique physical properties such as extreme surface wettability, which are potentially applicable for many purposes including water treatment. Laser-induced graphene (LIG) is an electrically conductive three-dimensional porous carbon material prepared by direct laser writing on various polymers in ambient conditions with a CO2 laser. Low-fouling LIG coatings in water technology have been reported; however, the mechanical strength and the separation properties of LIG-coated membranes are limited. Here, we show mechanically robust electrically conductive LIG-poly(vinyl alcohol) (PVA) composite membranes with tailored separation properties suitable for ultrafiltration processes. PVA has outstanding chemical and physical stability with good film-forming properties and is a biocompatible and nontoxic polymer. Compared to LIG-coated filters, the PVA-LIG composite membrane filters exhibited up to 63% increased bovine serum albumin rejection and up to ∼99.9% bacterial rejection, which corresponded well to the measured molecular weight cutoff ∼90 kDa. Compared to LIG fabricated on a polymer membrane control, the composite membranes showed similar excellent antifouling properties including low protein adsorption, and the antibiofilm effects were more pronounced at lower PVA concentrations. Notably for the antibacterial capabilities, the LIG-supporting layer maintained its electrical conductivity and a selected LIG-PVA composite used as electrodes showed complete elimination of mixed bacterial culture viability and indicated that the potent antimicrobial killing effects were maintained in the composite. This work demonstrates that the use of LIG for practical industrial filtration applications is possible.

Laser-Induced Graphene Composites as Multifunctional Surfaces.

Laser-induced graphene (LIG) is a platform material for numerous applications. Despite its ease in synthesis, LIG's potential for use in some applications is limited by its robustness on substrates. Here, using a simple infiltration method, we develop LIG composites (LIGCs) with physical properties that are engineered on various substrate materials. The physical properties include surface properties such as superhydrophobicity and antibiofouling; the LIGCs are useful in antibacterial applications and Joule-heating applications and as resistive memory device substrates.

Sulfur-Doped Laser-Induced Porous Graphene Derived from Polysulfone-Class Polymers and Membranes.

Graphene based materials have profoundly impacted research in nanotechnology, and this has significantly advanced biomedical, electronics, energy, and environmental applications. Laser-induced graphene (LIG) is made photothermally and has enabled a rapid route for graphene layers on polyimide surfaces. However, polysulfone (PSU), poly(ether sulfone) (PES), and polyphenylsulfone (PPSU) are highly used in numerous applications including medical, energy, and water treatment and they are critical components of polymer membranes. Here we show LIG fabrication on PSU, PES, and PPSU resulting in conformal sulfur-doped porous graphene embedded in polymer dense films or porous substrates using reagent- and solvent-free methods in a single step. We demonstrate the applicability as flexible electrodes with enhanced electrocatalytic hydrogen peroxide generation, as antifouling surfaces and as antimicrobial hybrid membrane-LIG porous filters. The properties and surface morphology of the conductive PSU-, PES-, and PPSU-LIG could be modulated using variable laser duty cycles. The LIG electrodes showed enhanced hydrogen peroxide generation compared to LIG made on polyimide, and showed exceptional biofilm resistance and potent antimicrobial killing effects when treated with Pseudomonas aeruginosa and mixed bacterial culture. The hybrid PES-LIG membrane-electrode ensured complete elimination of bacterial viability in the permeate (6 log reduction), in a flow-through filtration mode at a water flux of ∼500 L m-2 h-1 (2.5 V) and at ∼22 000 L m-2 h-1 (20 V). Due to the widespread use of PSU, PES, and PPSU in modern society, these functional PSU-, PES-, and PPSU-LIG surfaces have great potential to be incorporated into biomedical, electronic, energy and environmental devices and technologies.

Laser-Induced Graphene Layers and Electrodes Prevents Microbial Fouling and Exerts Antimicrobial Action.

Prevention of fouling on surfaces is a major challenge that broadly impacts society. Water treatment technologies, hospital infrastructure, and seawater pipes exemplify surfaces that are susceptible to biofouling. Here we show that laser-induced graphene (LIG) printed on a polyimide film by irradiation with a CO2 infrared laser under ambient conditions is extremely biofilm resistant while as an electrode is strongly antibacterial. We investigated the antibacterial activity of the LIG surface using LIG powder in suspension or deposited on surfaces, and its activity depended on the particle size and oxygen content. Remarkably, the antimicrobial effects of the surface were greatly amplified when voltages in the range of 1.1-2.5 were applied in an electrode configuration in bacterial solutions. The bactericidal mechanism was directly observed using microscopy and fast photography, which showed a rapid bacterial movement toward the LIG surface and subsequent bacterial killing. In addition, electrochemical generation of H2O2 was observed; however, the bacterial killing mechanism depended strongly on the physical and electrical contact of the bacterial cells to the surfaces. The anti-biofilm activity of the LIG surfaces and electrodes could lead to efficient protection of surfaces that are susceptible to biofouling in environmental applications by incorporating LIG onto the surfaces.

Impact of addition of amendments on the degradation of DDT and its residues partitioned on soil.

Market-grade DDT used for mosquito control and other purposes is a mixture of 4,4-DDT, 2,4-DDT and smaller amounts of 4,4-DDD, 2,4-DDD, 4,4-DDE and 4,4-DDMU. All above components (together known as DDTr) are strongly hydrophobic and hence are present in the environment predominantly in the soil/sediment phases. The persistence of DDTr and the feasibility of attenuation of DDTr concentration in soil matrix through addition of amendments is a subject of ongoing interest. The objective of this study was to compare the decline of soil-partitioned DDTr concentration through, (1) the natural attenuation process, (2) enhanced aerobic and anaerobic biodegradation processes involving addition of acclimatized seed and co-metabolites and (3) Nanoscale Zero Valent Iron (NZVI) addition. The extent of decline in soil DDTr concentration in control experiments, where biodegradation and photolysis were excluded, was around 10-15% in ∼100d. Extent of DDTr decline in natural attenuation experiments was 25-30% and 15-20% under aerobic and anaerobic conditions respectively. In enhanced biodegradation experiments, addition of acclimatized seed and/or co-metabolites did not enhance the extent of DDTr attenuation over and above the natural attenuation rates both in aerobic and anaerobic conditions. It thus appeared that biodegradation of DDTr adsorbed on soil was severely limited and controlled by desorption and consequent bioavailability of DDTr in the aqueous phase. In case of NZVI addition, the rate of DDTr degradation was much faster, with 40% decrease in DDTr concentration within 28h of NZVI addition. Here, the faster DDTr degradation may be through direct electron transfer between NZVI particles and DDTr molecules adsorbed on soil. Increase in the concentration of 4,4-DDD and 2,4-DDD during NZVI addition suggest that these compounds are either intermediate or end products of DDT degradation process.