Asunto(s)
Articulación de la Cadera , Histiocitosis de Células de Langerhans/diagnóstico , Niño , Diagnóstico Diferencial , Articulación de la Cadera/diagnóstico por imagen , Histiocitosis de Células de Langerhans/patología , Humanos , Imagen por Resonancia Magnética , Masculino , Tomografía Computarizada por Rayos XRESUMEN
The application of new technology to the diagnosis of infectious disease in neonates has markedly increased the potential for early clinical intervention. Although far from perfect, the new tests have increased knowledge of the nature of these diseases and give physicians the opportunity to apply current treatments more effectively as well as to develop new modes of therapy for this important class of diseases.
Asunto(s)
Enfermedades Fetales/diagnóstico , Enfermedades del Recién Nacido/diagnóstico , Infecciones Bacterianas/diagnóstico , Técnicas de Laboratorio Clínico , Femenino , Humanos , Recién Nacido , Embarazo , Toxoplasmosis/diagnóstico , Virosis/diagnósticoRESUMEN
The purpose of this study was to develop an enzyme-linked antiglobulin test (ELAT) for IgG on RBC without the hemolysis caused by the high pH of the alkaline phosphatase reaction. This was achieved by fixing the RBC with 0.05% glutaraldehyde after attachment of the antibodies. Assays using anti-D reference standards demonstrated the sensitivity to be 1-2 ng of antibody as compared to 7.5 ng for the manual indirect antiglobulin test. The coefficients of variation of these assays ranged from 10.4 to 20.1%. The mean background absorbance at 405 nm of 105 normal RBC samples was 0.08 +/- 0.03 SD. There was an increase in sensitivity of the test after the fixed RBC were stored. Dilute glutaraldehyde stabilizes the RBC membrane and the antigen-antibody linkage resulting in a more sensitive ELAT.