Effect of C/N substrates for enhanced extracellular polymeric substances (EPS) production and Poly Cyclic Aromatic Hydrocarbons (PAHs) degradation.

Extracellular Polymeric Substances (EPS) influenced Poly Cyclic Aromatic Hydrocarbons (PAHs) degrading Klebsiella pneumoniae was isolated from the marine environment. To increase the EPS production by Klebsiella pneumoniae, several physicochemical parameters were tweaked such as different carbon sources (arabinose, glucose, glycerol, lactose, lactic acid, mannitol, sodium acetate, starch, and sucrose at 20 g/L), nitrogen sources (ammonium chloride, ammonium sulphate, glycine, potassium nitrate, protease peptone and urea at 2 g/L), different pH, carbon/nitrogen ratio, temperature, and salt concentration were examined. Maximum EPS growth and biodegradation of Anthracene (74.31%), Acenaphthene (67.28%), Fluorene (62.48%), Naphthalene (57.84%), and mixed PAHs (55.85%) were obtained using optimized conditions such as glucose (10 g/L) as carbon source, potassium nitrate (2 g/L) as the nitrogen source at pH 8, growth temperature of 37 °C, 3% NaCl concentration and 72 h incubation period. The Klebsiella pneumoniae biofilm architecture was studied by confocal laser scanning microscopy (CLSM) and scanning electron microscope (SEM). The present study demonstrates the EPS influenced PAHs degradation of Klebsiella pneumoniae.

Comparison of integrated sustainable biodiesel and antibacterial nano silver production by microalgal and yeast isolates.

Microalgal isolates (Chlorella sp. and Spirulina sp.) and yeast isolates (Candida albicans and Saccharomyces sp.) were employed as the resources of biodiesel production and silver nanoparticle synthesis. The prominent peaks of the FTIR spectrum accustomed the efficient lipid property. The developed profile containing fatty acid methyl ester (FAME) displayed the elevated amount of both saturated (C15:0, C17:0, C21:0) and unsaturated (C17:1, C18:2, C20:4) fatty acids. The physicochemical properties analyzed by using Biodiesel analyzer V1.1.software, confirmed the competency of the isolates for sustainable biodiesel production. Biosynthesis of silvernanoparticles (AgNPs) were accomplished extracellularly by using supernatant of microalgal and yeast culture. The maximum absorbance at 420 and 421 nm under UV-visible spectra showed the presence of nanoparticles. The purity of the synthesized AgNPs were analyzed by XRD analysis. The elemental silver presence was affirmed by EDAX, SEM and AFM, the results revealed spherical crystalline shaped nanoparticles of size ranging from 2.0 to 7.3 nm. The antimicrobial efficacy of the silver nanoparticles (AgNPs) against various clinical pathogens which includes Bacillus sp., E. coli, Klebsiella sp., Proteus sp. and Staphylococcus aureus were observed. However, enhanced antimicrobial activity was displayed by the AgNPs, produced by Candida albicans (12 mm) against Bacillus sp., and E.coli, the nanoparticle produced by Chlorella sp. showed the least antagonistic activity (07 mm).

Exploring multi potential uses of marine bacteria; an integrated approach for PHB production, PAHs and polyethylene biodegradation.

There are copious of bacteria exist in marine environment and it is very important to screen the potential microbes that has the ability to produce biopolymer polyhydroxybutyrate (PHB) as well as polycyclic aromatic hydrocarbons (PAHs) degradation and conventional plastic high density polyethylene (HDPE) biodegradation. Numerous studies have been investigated individually on either one of characteristic feature like PHB production, PAHs and high density polyethylene (HDPE) degradation, but not all together. Hence, in this study, we tried to screen potential marine microbes that have the ability to perform all three features. We have isolated 203 phenotyphicaly different colonies from 19 different sites (marine soil sediments, marine water and oil spilled marine water) which cover the north east to down south seashore regions of Tamilnadu, India. Of the 203 microbial isolates, the best PHB producing (Micrococcus luteus), PAHs degradation (Klebsiella pneumonia) and HDPE degradation (Brevibacillus borstelensis) microorganisms were identified through 16S rRNA sequencing. Analytical studies confirmed PHB production by fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (1H &13C NMR); PAHs degradation by high performance liquid chromatography (HPLC), confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM); HDPE degradation by CLSM, FT-IR and SEM which cover the spectroscopy studies on biological systems.