How many microplastic particles are present in Canadian biosolids?

Application of treated sewage sludge (biosolids) from wastewater treatment plants (WWTPs) to farmlands is an important pathway through which microplastic particles (MPs) enter terrestrial ecosystems. Yet, microplastic concentrations in Canadian biosolids have only been estimated in samples from four WWTPs previously. We aimed to fill this knowledge gap by quantifying microplastics in biosolids from 22 WWTPs located in nine provinces and two commercial fertilizer producers in Canada. All samples had substantial microplastic concentrations ranging from 228 to 1353 particles per gram dry weight (median = 636 particles), which are orders of magnitude greater than MPs reported from earlier investigations of biosolids from other countries. Fibers (median: 86%) were the most common type of MPs observed, followed by fragments (median: 13%). There were no statistically significant differences in the amount of microplastics observed in the biosolids from different geographical regions, WWTP types, and sludge treatment processes. This suggests that diverse combinations of local sewershed characteristics, site-specific treatment approaches, and daily flow at WWTPs may be influencing concentrations of microplastics in biosolids. Our results indicate that microplastic concentrations in biosolids are substantially higher than they are in other environmental matrices, and this has important implications to managing microplastic pollution in terrestrial ecosystems.

Eutrophication and climatic changes lead to unprecedented cyanobacterial blooms in a Canadian sub-Arctic landscape.

Cyanobacterial blooms have been increasing in frequency and intensity but are often considered an issue restricted to temperate and tropical lakes. Here we report on one of the first occurrences of recurring cyanobacterial (Planktothrix spp.) blooms in a sub-Arctic lake from Yellowknife (Northwest Territories, Canada) and provide a long-term environmental context for the recent blooms using local meteorological data and multi-proxy paleolimnological analyses. Multiple co-occurring regional (gold mining emissions and climatic change) and local (land clearance and urbanization) stressors have impacted Jackfish Lake during the 20th and early-21st centuries, which have led to biological responses across multiple trophic levels. The unprecedented post-2013 cyanobacterial blooms were likely a cumulative response to nutrient enrichment and complex climate-mediated changes to lake thermal properties. A regional analysis of eight lakes around Yellowknife revealed that reduced ice cover duration and longer growing seasons have led to an increase in whole-lake primary production, whilst urban lakes were also fertilized by nutrients from local land-use changes in their catchments. Our findings suggest that anthropogenically nutrient-enriched sub-Arctic lakes, akin to their lower-latitude counterparts, may be vulnerable to cyanobacterial blooms in a warming world.

Impacts on aquatic biota from salinization and metalloid contamination by gold mine tailings in sub-Arctic lakes.

Precious metal mining activities have left complex environmental legacies in lakes around the world, including some sites in climatically sensitive regions of the Canadian sub-Arctic. Here, we examined the long-term impacts of past regional gold mining activities on sub-Arctic lakes near Con Mine (Yellowknife, Northwest Territories) based on sediment core analysis (paleolimnology). In addition to receiving metal(loid)s from roaster stack emissions, the study lakes were also influenced by salt-rich mine drainage from Con Mine tailings. Water samples from these lakes had some of the highest concentrations for salinity-related variables (e.g. Ca2+, Cl-, Na+) and metal(loid)s (e.g. As, Cu, Ni, Sb) in the Yellowknife area. Furthermore, the presence of halophilic diatom (Bacillariophyceae) taxa (Achnanthes thermalis and Navicula incertata) in the recent sediments of Keg and Peg lakes suggest that the extreme saline conditions are strongly influencing the present biota, more than 10 years after the cessation of gold mining activities at Con Mine. The sedimentary metal(loid) profiles (e.g. As, Cu, Ni) of Kam Lake tracked the influence of regional gold mining activities, particularly those at Con Mine, while the algal assemblages recorded the biological responses to salinization and metal(loid) pollution (e.g. marked decreases in diatom species richness, Hill's N2 diversity, and chrysophyte cyst:diatom valve ratio). At Kam Lake, the algal assemblage changes in the post-mining era were indicative of climate-mediated changes to lake thermal properties (e.g. rise in planktonic diatoms), nutrient enrichment related to urbanization (e.g. increase in eutrophic Stephanodisucs taxa), and/or a combination of both stressors. The lack of biological recovery (i.e. return to pre-mining assemblages) is consistent with investigations of mine-impacted lakes in temperate regions where elevated contaminant levels and emerging stressors (e.g. climate warming, land-use changes) are influencing lake recovery.

The impacts of century-old, arsenic-rich mine tailings on multi-trophic level biological assemblages in lakes from Cobalt (Ontario, Canada).

Silver mining in the early-1900s has left a legacy of arsenic-rich mine tailings around the town of Cobalt, in northeastern Ontario, Canada. Due to a lack of environmental control and regulations at that time, it was common for mines to dispose of their waste into adjacent lakes and land depressions, concentrating metals and metalloids in sensitive aquatic ecosystems. In order to examine what impacts, if any, these century-old, arsenic-rich mine tailings are having on present-day aquatic ecosystems, we sampled diatom assemblages in lake surface sediment in 24 lakes along a gradient of surface water arsenic contamination (0.4-972 µg/L). In addition, we examined sedimentary Cladocera and chironomid abundances and community composition, as well as open-water zooplankton communities and chlorophyll-a concentrations in10 of these study lakes along a gradient of arsenic contamination (0.9-1113 µg/L). Our results show that present-day arsenic concentration is not a significant driver of biotic community composition of the organisms we studied, but instead, that other variables such as lake depth and pH were more important in structuring assemblages. These results suggest that, while legacy contamination has greatly increased metal concentration beyond historical conditions, variability in lake-specific controls among the study lakes appear to be more important in the structuring of diatom, Cladocera, chironomidae, and zooplankton in these lakes.

Tracking pesticide use in the Saint Lawrence River and its ecological impacts during the World Exposition of 1967 in Montreal, Canada.

We analyzed dated sediment cores for evidence of Rhothane (dichlorodiphenyldichloroethane; DDD) applications to the Saint Lawrence River at Montreal, QC, Canada for the World Exposition of 1967 (Expo 67). More than 16,000kg of this pesticide were applied between 1965 and 1967 to abate nuisance shadflies that threatened visitor enjoyment. Concentrations of DDD and DDE in Lake Saint-François, 70km upstream of Expo 67, reached 12.2 and 11.5µg/kg dry weight (dw), respectively, with clear peaks between 1945 and 1970, consistent with historical use patterns; DDT was not detected. In Lake Saint-Pierre, ~100km downstream, DDD and DDE concentrations were 2 to 5 times higher, exceeding sediment quality guidelines, and DDT concentrations were as high as 3.8µg/kg. Once normalized for grain size and organic carbon, peaks of DDD, DDE and DDT were observed between 1945 and 1990 in the sediment record. Ratios of DDD to DDE were 1.0 or less in Lake Saint-François, consistent with their formation as degradation products of DDT. In contrast, ratios exceeded 1.0 in Lake Saint Pierre between 1965 and 1970, coinciding with Rhothane applications at Expo 67. Downstream, subfossil diatom assemblages showed little response to DDD inputs, but the abundance of some chironomid taxa increased while others decreased in tandem with elevated DDD concentrations. Overall, contamination of river sediments and impacts on insect communities by DDD applications at Expo 67 were still evident in sediment records 100km downstream of Montreal.