RESUMEN
Biohydrogen production from marine macroalgal biomass by advanced pre-treatment strategies is considered a clean energy technology. The present study focuses on investigating the effects of sonication pre-treatment (SP) and saponin coupled sonic pre-treatment (SSP) on Ulva fasciata for enhancing the production of biohydrogen. The SP and SSP were optimized to improve the hydrolysis process during digestion. The optimized time and sonication power were found respectively as 30 min and 200 W. A high concentration of biopolymer release was noticed in SSP than SP at optimized conditions. The surfactant dosage in SSP was optimized at 0.0036 g/g TS. The effect of SSP process was assessed by estimation of COD (Chemical Oxygen Demand) and SCOD (Soluble Chemical Oxygen Demand) release. The study revealed that, at a specific energy of 36,000 KJ/Kg TS, the SCOD release was higher in SSP (1900 mg/L) than SP (1050 mg/L). The SSP process could improve the COD solubilization to 15 % more than the SP. Carbohydrate and protein release are also more in SSP than SP. The use of biosurfactants significantly reduced the energy utilization in the hydrolysis process. The SSP pre-treated Ulva fasciata biomass has yielded a higher biohydrogen of 91.7 mL/g COD which is higher compared to SP (40.5 mL/g COD) and Control (9 mL/g COD).