Asunto(s)
Condroma/diagnóstico por imagen , Rodilla/diagnóstico por imagen , Neoplasias de los Tejidos Blandos/diagnóstico por imagen , Adulto , Condroma/patología , Condroma/cirugía , Humanos , Masculino , Radiografía , Neoplasias de los Tejidos Blandos/patología , Neoplasias de los Tejidos Blandos/cirugíaRESUMEN
The qualitative features of the intracytoplasmic vacuoles occurring in the cells of pleural malignant mesothelioma and of metastatic carcinoma exfoliated in pleural fluid were studied. There were important differences in the two types of vacuoles. In mesotheliomas, the vacuoles were centrally situated, small and regular in size; these vacuoles stained positively with fat stains and always negatively with the periodic acid-Schiff stain. In routine cytology, these lipid-containing vacuoles can best be seen in air-dried May-Grünwald-Giemsa-stained smears and are almost invisible in wet-fixed Papanicolaou-stained smears. In metastatic carcinoma, the vacuoles were irregular in size, noncentral in location and often positive with mucin stains. Electron microscopy was used in one case to further elucidate the staining patterns of the vacuoles. This study proves that the analysis of the qualitative features of vacuoles of malignant cells exfoliated in pleural fluid can be of great value in the distinction between metastatic carcinoma and mesothelioma.
Asunto(s)
Mesotelioma/patología , Metástasis de la Neoplasia/patología , Derrame Pleural , Histocitoquímica , Humanos , Métodos , Microscopía ElectrónicaRESUMEN
Bronchi biopsy specimens of 42 patients with chronic respiratory diseases were studied for immunoglobulin-containing cells, using an indirect immunoperoxidase technique. The number of immunocytes was expressed per unit tissue section surface. The cells were located directly under the epithelium. In most biopsy specimens IgA-, IgG- and IgM-containing cells were demonstrable, with IgA- and IgG-containing cells in the majority. IgE-containing cells were found only in small numbers in biopsy specimens of a few patients. The presence of IgE-containing cells was nearly always accompanied by a high amount of mast cell-bound IgE in the bronchial mucosa. No correlation could be found between the number and class of immunocytes and clinical data.