RESUMEN
A 12-year-old Japanese boy had ectopic eruption of the permanent maxillary canines and permanent mandibular second molars accompanied by root resorption of the adjacent permanent teeth. The permanent maxillary lateral incisors were extracted, and fenestration traction treatment was performed for the ectopically erupting canines. They were moved to the occlusal position orthodontically using a lingual arch appliance and sectional arch wire. The mandibular second molars were moved distally with an appliance designed to minimize the load on the teeth. The treatment is discussed with consideration of the diagnosis and appliances.
Asunto(s)
Resorción Radicular/etiología , Erupción Ectópica de Dientes/complicaciones , Niño , Diente Canino/patología , Humanos , Incisivo/cirugía , Masculino , Mandíbula , Maxilar , Diente Molar/patología , Diseño de Aparato Ortodóncico , Extrusión Ortodóncica/instrumentación , Alambres para Ortodoncia , Extracción Dental , Técnicas de Movimiento Dental/instrumentaciónRESUMEN
A disintegrin and metalloprotease with thrombospondin type 1 motifs (ADAMTS) is a family of extracellular proteases and implicated in cleaving proteoglycans, such as aggrecan, versican and brevican. No information is available about expression or localization of these ADAMTSs in teeth. Versican is a large chondroitin sulfate proteoglycan that is present in a variety of connective tissue including dental pulp, dentin, cementum and periodontal ligaments. The present study was designed to investigate expression of ADAMTSs and versican during rat tooth eruption. Rat maxillary first molars in weeks 1, 2, 3, 4 and 6 were examined. The mRNA expression of ADAMTS1, ADAMTS4, ADAMTS5 and versican was localized using in situ hybridization. ADAMTS1, ADAMTS4, ADAMTS5 and versican were expressed in dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes. The temporal and spatial expression pattern in these cellular phenotypes was comparable among ADAMTSs and versican. The present study suggests that dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes may be involved in both production and degradation of versican with secreting ADAMTS1, ADAMTS4 and ADAMTS5.
Asunto(s)
Proteínas ADAM/genética , Erupción Dental/genética , Versicanos/genética , Animales , Cemento Dental/citología , Cemento Dental/metabolismo , Pulpa Dental/citología , Pulpa Dental/metabolismo , Regulación de la Expresión Génica , Hibridación in Situ , Masculino , Odontoblastos/citología , Odontoblastos/metabolismo , Ligamento Periodontal/citología , Ligamento Periodontal/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Corona del Diente/citología , Corona del Diente/metabolismoRESUMEN
Extracellular matrix (ECM) remodeling is achieved by both production and degradation of ECM molecules during bone development. ADAMTS (a disintegrin and metalloprotease with thrombospondin type 1 motifs) constitutes a family of extracellular proteases which are implicated in cleaving the protein versican. The present study was designed to investigate the expression of versican and ADAMTS1, 4, and 5 mRNA during bone development in rat mandibles and hind limbs by RT-PCR and in situ hybridization. Versican was localized by immunohistochemistry. The process of bone development from day 14 postcoitum through week 6 postnatum was divided into the beginning of osteogenesis, woven bone, and lamellar bone stages. Versican protein was abundant in the woven bone matrix, but decreased in the lamellar bone matrix. Versican mRNA was prominent in some osteoblasts with corresponding localization of the cognate protein. The temporal and spatial mRNA expression pattern of ADAMTS1, 4, and 5 was comparable to that of versican. These results suggest that woven bone rich in versican alters into lamellar bone containing little versican during bone development in both mandibles and hind limbs, where some osteoblasts may be involved in production as well as degradation of versican by secreting ADAMTS1, 4, and 5.