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1.
Sensors (Basel) ; 23(23)2023 Dec 02.
Artículo en Inglés | MEDLINE | ID: mdl-38067941

RESUMEN

Vehicle type and brand information constitute a crucial element in intelligent transportation systems (ITSs). While numerous appearance-based classification methods have studied frontal view images of vehicles, the challenge of multi-pose and multi-angle vehicle distribution has largely been overlooked. This paper proposes an appearance-based classification approach for multi-angle vehicle information recognition, addressing the aforementioned issues. By utilizing faster regional convolution neural networks, this method automatically captures crucial features for vehicle type and brand identification, departing from traditional handcrafted feature extraction techniques. To extract rich and discriminative vehicle information, ZFNet and VGG16 are employed. Vehicle feature maps are then imported into the region proposal network and classification location refinement network, with the former generating candidate regions potentially containing vehicle targets on the feature map. Subsequently, the latter network refines vehicle locations and classifies vehicle types. Additionally, a comprehensive vehicle dataset, Car5_48, is constructed to evaluate the performance of the proposed method, encompassing multi-angle images across five vehicle types and 48 vehicle brands. The experimental results on this public dataset demonstrate the effectiveness of the proposed approach in accurately classifying vehicle types and brands.

2.
Genes (Basel) ; 14(3)2023 03 07.
Artículo en Inglés | MEDLINE | ID: mdl-36980935

RESUMEN

SSR (simple sequence repeat) DNA markers are widely used for genotype DNA identification, QTL mapping, and analyzing genetic biodiversity. However, SSRs in grapes are still in their early stages, with a few primer pairs accessible. With the whole-genome sequencing (WGS) of several grape varieties, characterization of grape SSR changed to be necessary not only to genomics but to also help SSR development and utility. Based on this, we identified the whole-genome SSR of nine grape cultivars ('PN40024', 'Cabernet Sauvignon', 'Carménère', 'Chardonnay', 'Merlot', 'Riesling', 'Zinfandel', 'Shine Muscat', and 'Muscat Hamburg') with whole-genome sequences released publicly and found that there are great differences in the distribution of SSR loci in different varieties. According to the difference in genome size, the number of SSRs ranged from 267,385 (Cabernet Sauvignon) to 627,429 (Carménère), the density of the SSR locus in the genome of nine cultivars was generally 1 per Kb. SSR motif distribution characteristic analysis of these grape cultivars showed that the distribution patterns among grape cultivars were conservative, mainly enriched in A/T. However, there are some differences in motif types (especially tetranucleotides, pentanucleotides, and hexanucleotides), quantity, total length, and average length in different varieties, which might be related to the size of the assembled genome or the specificity of variety domestication. The distribution characteristics of SSRs were revealed by whole-genome analysis of simple repeats of grape varieties. In this study, 32 pairs of primers with lower polymorphism have been screened, which provided an important research foundation for the development of molecular markers of grape variety identification and the construction of linkage maps of important agronomic traits for crop improvement.


Asunto(s)
Vitis , Vitis/genética , Genoma de Planta/genética , Mapeo Cromosómico , Polimorfismo Genético , Repeticiones de Microsatélite/genética
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