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1.
Biochem Genet ; 2024 Sep 19.
Artículo en Inglés | MEDLINE | ID: mdl-39300006

RESUMEN

Severe Corona Virus Disease 2019 (COVID-19) patients may develop acute respiratory distress syndrome (ARDS). Modified Ginseng Baidu Powder (referred to as Baidu Powder) was used for respiratory system diseases caused by colds. To study the effect of Baidu Powder on protecting ARDS mice model and its underlying active ingredients and targets intervening in COVID-19. The optimal LPS concentration was selected for the induction of mouse ARDS model, and the protective effect of Baidu Powder prophylactic administration on LPS-induced ARDS mouse models was explored by mouse survival time analysis, lung wet/dry weight (W/D) ratio, pathological staining, and inflammatory factor detection. On the basis of pharmacodynamics, the network pharmacological analysis was used for target prediction for future mechanism study. 5 mg/kg LPS was selected for the construction of a mouse ARDS model, based on a mortality rate of 87% and the lung W/D ratio of 5.29 ± 0.23. Prophylactic administration of Baidu Powder at 125 g/L significantly reduced death, lung damage, inflammatory cell infiltration, and cytokine production (TNF-α, IL-6, and IL-10) caused by LPS-induced ARDS. The results of network pharmacological analysis showed that 42 target genes of Baidu Powder intervening in COVID-19 were involved in 30 biological processes related to COVID-19 and inflammation, and 11 signaling pathways related to lung diseases or inflammation. 5 mg/kg LPS can successfully establish a mice ARDS disease model; 125 g/L Baidu Powder prophylactic administration does not have toxicity and has a certain effect on protecting ARDS mouse models induced by LPS. Baidu Powder may intervene COVID-19-induced ARDS through multiple targets.

2.
Virol J ; 21(1): 222, 2024 Sep 19.
Artículo en Inglés | MEDLINE | ID: mdl-39300471

RESUMEN

BACKGROUND: Viral diseases of sweet potatoes are causing severe crop losses worldwide. More than 30 viruses have been identified to infect sweet potatoes among which the sweet potato latent virus (SPLV), sweet potato mild speckling virus (SPMSV), sweet potato virus G (SPVG) and sweet potato virus 2 (SPV2) have been recognized as distinct species of the genus Potyvirus in the family Potyviridae. The sweet potato virus 2 (SPV2) is a primary pathogen affecting sweet potato crops. METHODS: In this study, we detected an SPV2 isolate (named SPV2-LN) in Ipomoea nil in China. The complete genomic sequence of SPV2-LN was obtained using sequencing of small RNAs, RT-PCR, and RACE amplification. The codon usage, phylogeny, recombination analysis and selective pressure analysis were assessed on the SPV2-LN genome. RESULTS: The complete genome of SPV2-LN consisted of 10,606 nt (GenBank No. OR842902), encoding 3425 amino acids. There were 28 codons in the SPV2-LN genome with a relative synonymous codon usage (RSCU) value greater than 1, of which 21 end in A/U. Among the 12 proteins of SPV2, P3 and P3N-PIPO exhibited the highest variability in their amino acid sequences, while P1 was the most conserved, with an amino acid sequence identity of 87-95.3%. The phylogenetic analysis showed that 21 SPV2 isolates were clustered into four groups, and SPV2-LN was clustered together with isolate yu-17-47 (MK778808) in group IV. Recombination analysis indicated no major recombination sites in SPV2-LN. Selective pressure analysis showed dN/dS of the 12 proteins of SPV2 were less than 1, indicating that all were undergoing negative selection, except for P1N-PISPO. CONCLUSION: This study identified a sweet potato virus, SPV2-LN, in Ipomoea nil. Sequence identities and genome analysis showed high similarity between our isolate and a Chinese isolate, yu-17-47, isolated from sweet potato. These results will provide a theoretical basis for understanding the genetic evolution and viral spread of SPV2.


Asunto(s)
Uso de Codones , Genoma Viral , Ipomoea , Filogenia , Enfermedades de las Plantas , Potyvirus , Enfermedades de las Plantas/virología , Ipomoea/virología , Potyvirus/genética , Potyvirus/clasificación , Potyvirus/aislamiento & purificación , China , ARN Viral/genética , Recombinación Genética , Análisis de Secuencia de ADN , Ipomoea batatas/virología , Secuenciación Completa del Genoma
3.
ACS Sens ; 2024 Sep 20.
Artículo en Inglés | MEDLINE | ID: mdl-39300913

RESUMEN

Wearable thermoelectric (TE) materials are seen as excellent candidates for flexible electronics because of their unique self-powered properties, multistimulus sensing and human waste heat conversion. However, currently reported flexible TE materials still face challenges such as poor durability, uncomfortable wearing and sensing signals crosstalking each other. Herein, this study describes a hot-air cross-linking method for the preparation of multifunctional TE fabrics with enhanced durability. Poly(ethylene terephthalate) (PET) fibers with core and sheath structures having different melting points were selected as flexible substrates. Poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) and single-walled carbon nanotubes (SWCNTs) were embedded stably on the surface of the sheath layer in the presence of heat treatment. The fiber-welded structure created by thermal cross-linking improves the durability of TE fabrics, including consistent mechanical and electrical properties after a 6 h wash test and 6000 compression cycles. The unique fiber structure of TE fabrics ensures excellent breathability (313.7 mm s-1 at 200 Pa), which meets the breathability requirements for human wear. In addition, the fiber-prepared sensors have excellent compressive strain response (20 ms response time and 30 ms recovery time) and precise temperature discrimination (0.17 K minimum discrimination temperature) for accurate real-time monitoring of the sensed signals. Thus, the TE fabrics can be used for human motion recognition, including pulse monitoring, sign language expression, and motions in joint areas. Moreover, the fabricated wearable TE device is connected to a Bluetooth module for wireless transmission, which can be used for mechanical and temperature sensing of the robot arm without signals crosstalking. This new durable TE fabric paves the way for the next generation of smart wearable technology.

4.
Org Lett ; 26(37): 7920-7925, 2024 Sep 20.
Artículo en Inglés | MEDLINE | ID: mdl-39248657

RESUMEN

A cooperative Rh(II)/Pd(0) dual-catalysis strategy that enabled divergent reactions of α-diazo 1,3-dicarbonyl compounds with allylic carbonates involving ketene versus carbene intermediates is described. The efficient synthesis of α-quaternary allylated ß-keto-esters was accomplished by the Rh(II)/Pd(0) dual-catalysis allylic alkylation of α-diazo 1,3-dicarbonyl compounds. Alternatively, an unprecedented (1+4) annulation of α-diazo 1,3-dicarbonyl compounds with 2-(hydroxymethyl)allyl carbonates via Rh(II)/Pd(0) dual catalysis was also successfully developed, affording a wide variety of α-quaternary tetrahydrofurans in good to high yields.

5.
Nat Commun ; 15(1): 7738, 2024 Sep 04.
Artículo en Inglés | MEDLINE | ID: mdl-39232004

RESUMEN

China faces challenges in reaching its carbon neutrality goal by the year 2060 to meet the Paris Agreement and improving air quality simultaneously. Dramatic nitrogen emission reductions will be brought by this ambitious target, yet their impact on the natural ecosystem is not clear. Here, by combining two atmospheric chemistry models and two process-based terrestrial ecosystem models constrained using nationwide measurements, we show that atmospheric nitrogen deposition in China's terrestrial land will decrease by 44-57% following two emission control scenarios including one aiming at carbon neutrality. They consequently result in a pronounced shrinkage in terrestrial net ecosystem production, by 11-20% depending on models and emission scenarios. Our results indicate that the nitrogen emission reductions accompanying decarbonization would undermine natural carbon sinks and in turn set back progress toward carbon neutrality. This unintended impact calls for great concern about the trade-offs between nitrogen management and carbon neutrality.

6.
Stress Biol ; 4(1): 40, 2024 Sep 20.
Artículo en Inglés | MEDLINE | ID: mdl-39302547

RESUMEN

Aquilaria sinensis is a significant resin-producing plant worldwide that is crucial for agarwood production. Agarwood has different qualities depending on the method with which it is formed, and the microbial community structures that are present during these methods are also diverse. Furthermore, the microbial communities of plants play crucial roles in determining their health and productivity. While previous studies have investigated the impact of microorganisms on agarwood formation, they lack comprehensiveness, particularly regarding the properties of the microbial community throughout the entire process from seedling to adult to incense formation. We collected roots, stems, leaves, flowers, fruits and other tissues from seedlings, healthy plants and agarwood-producing plants to address this gap and assess the dominant bacterial species in the microbial community structures of A. sinensis at different growth stages and their impacts on growth and agarwood formation. The bacteria and fungi in these tissues were classified and counted from different perspectives. The samples were sequenced using the Illumina sequencing platform, and sequence analyses and species annotations were performed using a range of bioinformatics tools to assess the plant community compositions. An additional comparison of the samples was conducted using diversity analyses to assess their differences. This research revealed that Listeria, Kurtzmanomyces, Ascotaiwania, Acinetobacter, Sphingobium, Fonsecaea, Acrocalymma, Allorhizobium, Bacillus, Pseudomonas, Peethambara, and Debaryomyces are potentially associated with the formation of agarwood. Overall, the data provided in this article help us understand the important roles played by bacteria and fungi in the growth and agarwood formation process of A. sinensis, will support the theoretical basis for the large-scale cultivation of A. sinensis, and provide a basis for further research on microbial community applications in agarwood production and beyond.

7.
Exp Neurol ; 382: 114961, 2024 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-39288829

RESUMEN

Intracerebral hemorrhage, is a cerebrovascular disease with high morbidity, mortality, and disability. Due to the lack of effective clinical treatments, the development of new drugs to treat intracerebral hemorrhage is necessary. In recent years, ferroptosis has been found to play an important role in the pathophysiological process of intracerebral hemorrhage, which can be treated by inhibiting ferroptosis and thus intracerebral hemorrhage. This article aims to explain the mechanism of ferroptosis and its relationship to intracerebral hemorrhage. In the meantime, it briefly discusses the molecules identified to alleviate intracerebral hemorrhage by inhibiting ferroptosis, along with other clinical agents that are expected to treat intracerebral hemorrhage through this mechanism. In addition, a brief overview of the morphological alterations of different forms of cell death and their role in ICH is provided. Finally, the challenges that may arise in translating ferroptosis inhibitors from basic research to clinical use are presented. This article serves as a reference and provides insights to aid in the treatment of intracerebral hemorrhage in the clinic.

8.
Angew Chem Int Ed Engl ; : e202410848, 2024 Sep 13.
Artículo en Inglés | MEDLINE | ID: mdl-39268653

RESUMEN

There has been a growing interest in developing catalysts to enable the reversible iodine conversion reaction for high-performance aqueous zinc-iodine batteries (AZIBs). While diatomic catalysts (DACs) have demonstrated superior performance in various catalytic reactions due to their ability to facilitate synergistic charge interactions, their application in AZIBs remains unexplored. Herein, we present, for the first time, a DAC comprising Mn-Zn dual atoms anchored on a nitrogen-doped carbon matrix (MnZn-NC) for iodine loading, resulting in a high-performance AZIB with a capacity of 224 mAh g-1 at 1 A g-1 and remarkable cycling stability over 320,000 cycles. The electron hopping along the Mn-N-Zn bridge is stimulated via a spin exchange mechanism. This process broadens the Mn 3dxy band width and enhances the metallic character of the catalyst, thus facilitating charge transfer between the catalysts and reaction intermediates. Additionally, the increased electron occupancy within the d-orbital of Zn elevates Zn's d-band center, thereby enhancing chemical interactions between MnZn-NC and I-based species. Furthermore, our mechanism demonstrates potential applicability to other Metal-Zn-NC DACs with spin-polarized atoms. Our work elucidates a clear mechanistic understanding of diatomic catalysts and provides new insights into catalyst design for AZIBs.

9.
Polymers (Basel) ; 16(17)2024 Sep 08.
Artículo en Inglés | MEDLINE | ID: mdl-39274172

RESUMEN

In this paper, we developed a paper-based fluorescent sensor using functional composite materials composed of graphene quantum dots (GQDs) coated with molecularly imprinted polymers (MIPs) for the selective detection of tetracycline (TC) in water. GQDs, as eco-friendly fluorophores, were chemically grafted onto the surface of paper fibers. MIPs, serving as the recognition element, were then wrapped around the GQDs via precipitation polymerization using 3-aminopropyltriethoxysilane (APTES) as the functional monomer. Optimal parameters such as quantum dot concentration, grafting time, and elution time were examined to assess the sensor's detection performance. The results revealed that the sensor exhibited a linear response to TC concentrations in the range of 1 to 40 µmol/L, with a limit of detection (LOD) of 0.87 µmol/L. When applied to spiked detection in actual water samples, recoveries ranged from 103.3% to 109.4%. Overall, this paper-based fluorescent sensor (MIPs@GQDs@PAD) shows great potential for portable, multi-channel, and rapid detection of TC in water samples in the future.

10.
Cell Death Dis ; 15(9): 675, 2024 Sep 14.
Artículo en Inglés | MEDLINE | ID: mdl-39277583

RESUMEN

Rap2b, a proto-oncogene upregulated in colorectal cancer (CRC), undergoes protein S-palmitoylation at specific C-terminus sites (C176/C177). These palmitoylation sites are crucial for Rap2b localization on the plasma membrane (PM), as mutation of C176 or C177 results in cytosolic relocation of Rap2b. Our study demonstrates that Rap2b influences cell migration and invasion in CRC cells, independent of proliferation, and this activity relies on its palmitoylation. We identify ABHD17a as the depalmitoylating enzyme for Rap2b, altering PM localization and inhibiting cell migration and invasion. EGFR/PI3K signaling regulates Rap2b palmitoylation, with PI3K phosphorylating ABHD17a to modulate its activity. These findings highlight the potential of targeting Rap2b palmitoylation as an intervention strategy. Blocking the C176/C177 sites using an interacting peptide attenuates Rap2b palmitoylation, disrupting PM localization, and suppressing CRC metastasis. This study offers insights into therapeutic approaches targeting Rap2b palmitoylation for the treatment of metastatic CRC, presenting opportunities to improve patient outcomes.


Asunto(s)
Membrana Celular , Movimiento Celular , Neoplasias Colorrectales , Lipoilación , Proto-Oncogenes Mas , Proteínas de Unión al GTP rap , Humanos , Neoplasias Colorrectales/patología , Neoplasias Colorrectales/metabolismo , Neoplasias Colorrectales/genética , Proteínas de Unión al GTP rap/metabolismo , Proteínas de Unión al GTP rap/genética , Membrana Celular/metabolismo , Animales , Línea Celular Tumoral , Metástasis de la Neoplasia , Transducción de Señal , Ratones , Ratones Desnudos , Fosfatidilinositol 3-Quinasas/metabolismo , Receptores ErbB/metabolismo , Proliferación Celular
11.
Food Chem X ; 23: 101773, 2024 Oct 30.
Artículo en Inglés | MEDLINE | ID: mdl-39280223

RESUMEN

This study investigated the effects of Lycium barbarum pulp (LBP) on the properties of mixed dough and gluten protein. The results showed that appropriate addition of LBP (5 %) significantly improved the performance of the dough, promoted the aggregation of gluten protein, enhanced the water binding ability, and delayed the gelatinization of starch during cooking. Compared with the control group, the peak temperature (Tp) of the LBP sample gradually increased from 63.23 °C to 65.56 °C, the expansion force reduced by about 21.56 %, the absolute Zeta potential lowered by about 18.4 %, and the α -helix content and ß -folding increased by 32.36 % and 10.23 %, respectively, indicating the more orderly and stable overall structure. However, LBP did not change the crystal configuration of starch and still showed typical type A line diffraction. Moreover, the addition of LBP increased the polyphenol content, which further improved the antioxidant properties and provided the possibility to improve the health potential of the flour.

12.
Talanta ; 281: 126857, 2024 Sep 09.
Artículo en Inglés | MEDLINE | ID: mdl-39255620

RESUMEN

2,4,6-Trinitrotoluene (TNT) and its four metabolites, namely 2-ADNT, 4-ADNT, 2,4-DANT, and 2,6-DANT, are highly toxic substances. These metabolites also serve as biomarkers for assessing the health of individuals exposed to TNT. In this study, a homemade DDT-IMS apparatus was utilized to detect these metabolites. Under negative detection mode, the drift times of 2-ADNT and 4-ADNT showed subtle shifts within a drift tube temperature range of 100 °C-120 °C, aiding in their differentiation. In positive detection mode for 2,4-DANT and 2,6-DANT, significant variations were observed in both the number and drift time of their positive product ions across a drift tube temperature range of 80 °C-120 °C. Consequently, optimal analytical performance for these metabolites was achieved at approximately 100 °C. Evaluation of the instrumental response during the measurement of the four metabolites in both positive and negative modes revealed that negative detection mode offered greater advantages of detecting these compounds. The working ranges for measuring the four metabolites spanned two orders of magnitude, with detection limits for each metabolite nearly below 1 ng. Notably, clear identification of the signals for these metabolites was achieved even when samples were mixed in urine, highlighting the ability of the DDT-IMS in detecting TNT metabolites. The developed DDT-IMS detection method has significant potential for enhancing environmental risk assessment and biological hazard evaluation, particularly in relation to human exposure to TNT.

13.
Adv Healthc Mater ; : e2401419, 2024 Sep 10.
Artículo en Inglés | MEDLINE | ID: mdl-39252653

RESUMEN

Stem cell transplantation has demonstrated efficacy in treating neurological disorders by generating functional cells and secreting beneficial factors. However, challenges remain for current cell suspension injection therapy, including uncontrollable cell distribution, the potential for tumor formation, and limited ability to treat spatial defects. Therefore, implants with programmable cell development, tailored 3D structure, and functionalized biomaterials have the potential to both control cell distribution and reduce or heal spatial defects. Here, a biomimetic material system comprising gelatin, alginate, and fibrinogen has been developed for neural progenitor cell constructs using 3D printing. The resulting constructs exhibit excellent formability, stability, and developmental functions in vitro, as well as biocompatibility and integration into the hippocampus in vivo. The controllability, reproducibility, and material composition of the constructs show potential for use in personalized stem cell-based therapies for defective neurological disorders, neural development research, disease modeling, and organoid-derived intelligent systems.

14.
Biodivers Data J ; 12: e129087, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39229385

RESUMEN

Background: As a genus within the Clavicipitaceae, Metarhizium exhibits rich morphological and ecological diversity, with a wide distribution and a variety of hosts. Currently, sixty-eight species of Metarhizium have been described. New information: A new species of Metarhizium, M.puerense (Hong Yu bis), was described in Pu'er City, Yunnan Province, south-western China. Based on morphological characteristics and multilocus phylogenetic analyses, Metarhiziumpuerense was confirmed to be phylogenetically related to M.album, but was clearly separated and formed a distinct branch. In contrast, the host of Metarhiziumalbum was plants and leafhoppers and that lepidopteran larvae were the host of M.puerense. The diagnostic features of M.puerense were solitary to multiple stromata and smooth-walled, cylindrical with rounded apices conidia.

15.
Front Pharmacol ; 15: 1324509, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-39246661

RESUMEN

Background: Chinese patent medicines are specialty preparations in China that are produced using traditional prescriptions processed by modern pharmaceutical technology. They contain complex ingredients and much attention is paid to their clinical safety. Demonstrating the clinical safety of Chinese patent medicines containing toxic ingredients in modern pharmacological studies has become one of the urgent issues to be solved for the safe use of clinical medicines. Objectives: The aim of this research is to evaluate the safety of Chinese patent medicines containing toxic ingredients by applying the risk-benefit assessment method. Additionally, a database of 'toxic ingredients-toxic Chinese herbal medicines-adverse reactions' will be established to explore the relationship between toxic ingredients and adverse reactions. This will lay the foundation for the rational clinical use of Chinese patent medicines containing toxic ingredients. Methods: 1) Establish a database of 'toxic Chinese herbal medicines-toxic ingredients-toxic Chinese patent medicines' to count the Chinese patent medicines containing toxic ingredients in the 2020 edition of Chinese Pharmacopoeia. 2) Filtered the clinical studies, extracted the drug-related ADEs, and analyzed the characteristics and correlations of these ADEs. 3) Finally, this section summarizes the causes of ADEs related to Chinese patent medicines containing toxic ingredients and extracts the main risk factors to provide a reference for further study. Outcomes: 1) There are four main types of Chinese patent medicines containing toxic ingredients. These include medicines with diester aconitine metabolites, mineral composition, Araceae metabolites, and hydrogen cyanide. 2) Digestive system, skin and its appendages, and allergic reactions were the main types of ADEs related to four types of Chinese patent medicines containing toxic ingredients. 3) There are four primary risk factors associated with the clinical use of Chinese patent medicines containing toxic ingredients: medicine, medication, individual and regulatory factors.

16.
CNS Neurosci Ther ; 30(9): e70021, 2024 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-39258790

RESUMEN

BACKGROUND: Sepsis-associated encephalopathy (SAE) is a neuronal injury with poor prognosis. Mitochondrial dysfunction is critical in SAE development, and hydrogen gas (H2) has a protective effect on septic mice. This study aimed to investigate the effect of high concentration (67%) of H2 on SAE and whether it is related to mitochondrial biogenesis and mitochondrial dynamics. METHODS: A mouse sepsis model was induced by cecal ligation and puncture. The mice inhalated 67% H2 for 1 h at 1 and 6 h post-surgery, respectively. The 7-day survival rate was recorded. Cognitive function was assessed using the Y-maze test and Morris water maze test. Serum inflammatory factors, antioxidant enzymes, as well as mitochondrial function indexes including mitochondrial membrane potential (MMP) and ATP in the hippocampal tissue were evaluated 24 h after surgery. Mitochondrial dynamic proteins (DRP1 and MFN2) and biosynthetic proteins (PGC-1α, NRF2, and TFAM) in the hippocampal tissue were detected. Moreover, the morphology of mitochondria was observed by transmission electron microscopy. RESULTS: Inhalation of 67% H2 improved the 7-day survival rates and recognition memory function of septic mice, alleviated brain antioxidant enzyme activity (SOD and CAT), and reduced serum proinflammatory cytokine levels. H2 inhalation also enhanced the expression of MFN2 and mitochondrial biogenesis-related factors (PGC-1α, NRF2, and TFAM) and decreased the expression of fission protein (DRP1), leading to improvement in mitochondrial function, as evidenced by MMP and ATP levels. CONCLUSIONS: Inhalation of high concentration (67%) of H2 in septic mice improved the survival rate and reduced neuronal injury. Its mechanism might be mediated by enhancing mitochondrial biogenesis and mitochondrial dynamics.


Asunto(s)
Hidrógeno , Dinámicas Mitocondriales , Encefalopatía Asociada a la Sepsis , Animales , Encefalopatía Asociada a la Sepsis/tratamiento farmacológico , Ratones , Hidrógeno/farmacología , Hidrógeno/administración & dosificación , Hidrógeno/uso terapéutico , Dinámicas Mitocondriales/efectos de los fármacos , Masculino , Administración por Inhalación , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Sepsis/complicaciones , Sepsis/tratamiento farmacológico , Sepsis/metabolismo , Ratones Endogámicos C57BL , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Aprendizaje por Laberinto/efectos de los fármacos
17.
J Sport Health Sci ; : 100976, 2024 Sep 03.
Artículo en Inglés | MEDLINE | ID: mdl-39237062

RESUMEN

BACKGROUND: Deficits in quadriceps strength of the injured leg have been observed in patients following anterior cruciate ligament (ACL) reconstructions and may contribute to ACL re-injury risk. Single-leg forward hopping is a widely used task for assessing knee function in patients following ACL reconstructions as it has been shown not to be particularly challenging to the knee. This study aimed to quantify the effect of decreased quadriceps strength induced by a fatigue protocol on hopping performance and lower limb mechanics in single-leg forward, vertical, and backward hopping. METHODS: Thirty-four injury-free participants performed single-leg forward, vertical, and backward hopping on both legs pre- and post-fatigue, with 1 leg experiencing a fatigue protocol. Peak moments, power, and work of hip, knee, and ankle joints were quantified during the jumping phase. Hopping performance and bilateral asymmetries in performance were assessed. RESULTS: Single-leg backward hopping demonstrated the greatest knee moments, power, and work compared to forward and vertical hopping, regardless of leg and fatigue. Fatigue protocol resulted in significantly less knee moments, power, and work, and decreased performance of the fatigued leg among all tasks. Bilateral symmetries in hopping performance decreased in post-fatigue, with the greatest decrease in backward hopping. CONCLUSION: The greater sensitivity of the backward hopping to detect quadriceps fatigue suggests it may act as a better or at least an additional metric to evaluate quadriceps strength deficits. The findings may contribute to the development of a clinically applicable and valid strength assessment to monitor the rehabilitation progress in patients following ACL reconstructions.

18.
Nanoscale Adv ; 6(18): 4611-4624, 2024 Sep 10.
Artículo en Inglés | MEDLINE | ID: mdl-39263398

RESUMEN

We explore the novel photodecomposition capabilities of ß-Ga2O3 when augmented with reduced graphene oxide (rGO). Employing real-time spectroscopy, this study unveils the sophisticated mechanisms of photodecomposition, identifying an optimal 1 wt% ß-Ga2O3-rGO ratio that substantially elevates the degradation efficiency of Methylene Blue (MB). Our findings illuminate a direct relationship between the photocatalyst's composition and its performance, with the quantity of rGO synthesis notably influencing the catalyst's morphology and consequently, its photodegradation potency. The 1 wt% ß-Ga2O3-rGO composition stands out in its class, showing a notable 4.7-fold increase in CO production over pristine ß-Ga2O3 and achieving CO selectivity above 98%. This remarkable performance is a testament to the significant improvements rendered by our novel rGO integration technique. Such promising results highlight the potential of our custom-designed ß-Ga2O3-rGO photocatalyst for critical environmental applications, representing a substantial leap forward in photocatalytic technology.

19.
Heliyon ; 10(16): e35607, 2024 Aug 30.
Artículo en Inglés | MEDLINE | ID: mdl-39224360

RESUMEN

The human rights and mega sport events (SMEs) discourses gain massive importance and have grown immensely in recent years. Most of the time, the sports governance bodies are responsible for administration and management of these sport mega events. Keeping in consideration the situation regarding increasing involvement, the study focuses on the field of sport along with pleasure and basic rights. Through re-evaluation the commercial factors of sports, the mega governing bodies of sport (IOC & FIFA), and researchers discuss what is referred to commonly as Ruggie Principle, and both would be applied to IOC & FIFA practices, operation, and management regarding their events. This study is essential as it emphasizes the contribution and importance of carrying out human rights impact assessments, particularly in the context of hosting rights. The survey's findings reveal that human rights and due diligence assessments are integral components of organizations such as FIFA and the IOC. This study, being an empirical investigation, allowed us to arrive at some normative conclusions that had significant implications for practices and policy issues. Additionally, it laid the groundwork for future research in the crucial area of SMEs globally.

20.
J Proteomics ; 307: 105278, 2024 Sep 15.
Artículo en Inglés | MEDLINE | ID: mdl-39142625

RESUMEN

Depression is a prominent contributor to global disability. A growing body of data suggests that depression is associated with the pathophysiology of the medial prefrontal cortex (mPFC), but the underlying mechanisms remain poorly understood. Mice were subjected to chronic restraint stress (CRS) for 3 weeks to create depression models during this investigation. Protein tandem mass tag (TMT) quantification and LC-MS/MS analysis were conducted to examine proteome patterns. Afterwards, to further explore the enrichment of differential proteins and the signaling pathways involved, we annotated these differentially expressed proteins. We confirmed that CRS mice developed depression-like and anxiety-like behaviors. Among the 8081 measured proteins, a total of 15 proteins were found to be differentially expressed. These proteins exhibited functional enrichment in a variety of biological functions, and among these pathways, alterations in synaptic function and autophagy are noteworthy. In addition, we identified a differentially expressed protein called Wnt2b and found that CRS may disrupt synaptic plasticity by affecting the activation of the Wnt2b/ß-catenin pathway. Our findings showed depression-like behaviors in the CRS mouse model and molecular alterations in the mPFC, which may help explain the pathogenesis of depression and identify novel antidepressant medication targets. SIGNIFICANCE: Depression is a prevalent and frequent chronic mental illness and is now a significant contributor to global disability. In this study, we used chronic restraint stress to establish a mouse model of depression, and differentially expressed proteins in the medial prefrontal cortex of depressed model mice were detected by TMT proteomics. Our study verified the presence of altered synaptic function and excessive autophagy in the mPFC of CRS-induced mice from a proteomic perspective. Furthermore, we demonstrated that CRS may disrupt synaptic plasticity by affecting the activation of the Wnt2b/ß-catenin pathway, which may be a key link in the pathogenesis of depression and may provide new insights for identifying new antidepressant drug targets.


Asunto(s)
Depresión , Corteza Prefrontal , Proteómica , Restricción Física , Estrés Psicológico , Animales , Corteza Prefrontal/metabolismo , Ratones , Estrés Psicológico/metabolismo , Proteómica/métodos , Depresión/metabolismo , Masculino , Modelos Animales de Enfermedad , Proteoma/metabolismo , Ratones Endogámicos C57BL
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