Preclinical evaluation of oncolytic potential human rotavirus Wt 1-5 in gastric adenocarcinoma.

Despite advances in biomedical research, gastric cancer remains the leading cause of morbidity and mortality worldwide due to the limited efficacy of conventional therapies. In recent decades, oncolytic viruses have emerged as a biological therapeutic alternative to cancer due to their selectivity, effectiveness, and low toxicity. However, clinical trials have shown that developing a virus with selectivity for multiple tumor receptors and the ability to penetrate and diffuse through the tumor microenvironment to reactivate the immune system remains challenging. This study aimed to examine the oncolytic potential of tumor cell-adapted rotavirus Wt1-5 in gastric adenocarcinoma samples. This study focused on determining the propagation capacity of the RV Wt1-5 through the tumor and the importance of the expression of cell surface co-receptors, including integrin ß3, protein disulfide isomerase (PDI), and heat shock proteins (Hsp-90, -70, -60, -40, and Hsc 70), during infection of tumor cells. These proteins were found to be differentially expressed in tumor cells compared to adjacent non-tumor cells. Preincubation of gastric tumor cells with antibodies against these proteins decreased rotavirus infections, validating their importance in the binding and entry of RV Wt1-5 into tumor cells, as previously reported. Upon RV infection, apoptosis was one of the types of death that was observed. This was evidenced by evaluating the expression of CASP-3, -9, PARP, cytochrome C, Bax, Bid, p53, and Bcl-2, as well as observing morphological changes such as chromatin margination, nuclear condensation, and fragmentation. Finally, at 60 h.p.i, histological analysis revealed that oncolysis compromised the entire thickness of the tumor. Therefore, the results suggest that RV Wt1-5 could be a novel therapeutic agent co-adjuvant agent for conventional and targeted therapies in managing GC. Ex vivo infection of the tumor tissue model showed characteristics of an immune response that could be explored in future studies.

Pulp revascularization with and without platelet-rich plasma in two anterior teeth with horizontal radicular fractures: a case report.

Pulp revascularization is an alternative treatment in immature traumatized teeth with necrotic pulp. However, this procedure has not been reported in horizontal root fractures. This is a case report of a 9-year-old patient with multiple horizontal root fractures in 2 upper central incisors that were successfully treated with pulp revascularization. The patient presented for treatment 2 years after the initial trauma, and revascularization was attempted after the initial treatment with calcium hydroxide had failed. Prior to pulp revascularization, cone-beam computed tomography and autoradiograms demonstrated multiple horizontal fractures in the middle and apical thirds of the roots of the 2 affected teeth. Revascularization was performed in both teeth; platelet-rich plasma (PRP) was used in one tooth (#11) and the conventional method (blood clot) was used in the other tooth (#21). Clinical and radiographic follow-up over 4 years demonstrated pulp calcification in the PRP-treated tooth. Neither of the 2 teeth were lost, and the root canal calcification of tooth #11 was greater than that of tooth #21. This case suggests that PRP-based pulp revascularization may be an alternative for horizontal root fractures.

Protocolo de manejo de diente permanente avulsionado para el servicio de salud oral de la Fundación Hospital La Misericordia y la Facultad de Odontología de la Universidad Nacional de Colombia, sede Bogotá (2011) / Protocol for Treatment of Avulsed Permanent Tooth at the Dental Care Unit of the La Misericordia Hospital Foundation and the National University of Colombia Dental School, campus Bogotá (2011)

Objetivo: la presente guía se elaboró con el fin de actualizar y complementar la guía demanejo de trauma dentoalveolar de la Fundación Hospital de la Misericordia y la Facultadde Odontología de la Universidad Nacional de Colombia. Métodos: se realizó una búsquedade literatura internacional en diferentes bases de datos y se analizaron los protocolos másrecientes y con mejores resultados en cuanto a la disminución de la aparición y al progresode la reabsorción radicular por remplazo, principal complicación al ser reimplantado undiente permanente avulsionado. Resultados: con esta actualización se pretende sugerir lamodificación de la terapia medicamentosa intraconducto y el acondicionamiento radicular,al encontrarse mejores mecanismos de acción de los medicamentos utilizados actualmente.Se recomienda llevar a cabo una socialización y aplicación de esta guía de manejo dentro dela Facultad de Odontología de la Universidad Nacional de Colombia, como en el Servicio deSalud Oral de la Fundación Hospital de la Misericordia...

Objective: This guide was conducted to update and complement the current managementguide for dentoalveolar trauma at the La Misericordia Hospital Foundation and the NationalUniversity of Colombia Dental School. Methods: A search of international literature in differentdatabases was carried out as well as the analysis of the most recent protocols andwith better results for decreasing the occurrence of root resorption, the main complicationafter an avulsed permanent teeth has been replanted. Results: This update is intended tosuggest the modification of the intracanal drug therapy and root surface conditioning to findbetter mechanisms of action of drugs currently used. It is recommended to disseminate andimplement this guide at the National University of Colombia Dental School and the dentalcare unit of the La Misericordia Hospital Foundation...