Asunto(s)
Antineoplásicos/uso terapéutico , Linfoma de Células B de la Zona Marginal/diagnóstico , Biopsia , Diagnóstico Diferencial , Progresión de la Enfermedad , Endoscopía Gastrointestinal , Resultado Fatal , Estudios de Seguimiento , Humanos , Linfoma de Células B de la Zona Marginal/tratamiento farmacológico , Linfoma de Células B de la Zona Marginal/radioterapia , Masculino , Persona de Mediana Edad , Radioterapia AdyuvanteRESUMEN
Mantle cell lymphoma (MCL) is an IgM-expressing B cell lymphoma that originates from naive B cells and responds poorly to chemotherapy. We show here that several MCLs harbour isotype-switched subclones. Similar to the situation in normal B cells, in vitro stimulation of MCL cell lines with CD40 ligand (CD40L) and interleukin-4 induced expression of activation-induced cytidine deaminase (AID) and germline transcription at the immunoglobulin heavy chain gene locus. Additionally, the occurrence of switch-circle transcripts and mature IgG transcripts after stimulation indicated ongoing class-switch recombination in mantle cell lymphoma cell lines. Furthermore, stimulation of primary MCL cells in vitro induced expression of class-switched IgG mRNA in the tumour cells. Our data indicate that mantle cell lymphomas have retained the ability to undergo class-switch recombination if appropriate stimuli, such as the CD40 ligand, are provided.
Asunto(s)
Cambio de Clase de Inmunoglobulina/genética , Linfoma de Células del Manto/genética , Antígenos CD40/inmunología , Línea Celular Tumoral , Citidina Desaminasa/inmunología , Células Dendríticas Foliculares/inmunología , Genes de las Cadenas Pesadas de las Inmunoglobulinas/genética , Genes de las Cadenas Pesadas de las Inmunoglobulinas/inmunología , Humanos , Cambio de Clase de Inmunoglobulina/inmunología , Inmunoglobulina G/genética , Inmunoglobulina G/inmunología , Inmunohistoquímica/métodos , Interleucina-4/inmunología , Linfoma de Células del Manto/inmunología , Mutación/genética , Mutación/inmunología , ARN Mensajero/análisis , ARN Neoplásico/análisis , Recombinación Genética/genética , Recombinación Genética/inmunología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Transcripción Genética/genética , Transcripción Genética/inmunologíaRESUMEN
OBJECTIVE: To evaluate telomerase activity as a marker of lymphocyte proliferation in systemic lupus erythematosus (SLE). METHODS: CD19+, CD4+, and CD8+ lymphocytes were isolated from the peripheral blood of nine patients with SLE and nine healthy controls by means of magnetic bead-coupled antibodies and tested for telomerase activity with the TRAP assay. RESULTS: Telomerase activity was significantly increased in CD19+ B cells from patients with SLE. CD4+ and CD8+ T cells from lupus patients displayed increased mean telomerase activity, although the difference from normal controls did not reach statistical significance. CONCLUSIONS: Increased telomerase activity in the B and the T cell lineage might indicate activation and proliferation of these lymphocytes.
Asunto(s)
Subgrupos de Linfocitos B/enzimología , Lupus Eritematoso Sistémico/inmunología , Subgrupos de Linfocitos T/enzimología , Telomerasa/sangre , Subgrupos de Linfocitos B/patología , División Celular/inmunología , Femenino , Humanos , Lupus Eritematoso Sistémico/genética , Subgrupos de Linfocitos T/patología , Telómero/ultraestructuraRESUMEN
The comparison of the absolute figures of the tick-borne encephalitis morbidity and mortality rates in the Primorye Territory for 16-22 years with the climatic parameters and the characteristics of the natural focus in the key area has allowed one to reveal close relationship between the two above-mentioned epidemiological characteristics and such factors as the intensity of monsoon rains and the size of the population of large-toothed redback voles in the previous year. Multiple regression equations suitable for the prognosis of the morbidity and mortality levels in the territory for the coming year have been set up.