RESUMEN
Introduction: This study aims to determine the baseline seroprevalence of leptospirosis, a zoonotic and neglected disease, in people living with HIV (PWH) in Maputo, Mozambique, and to evaluate the relationship between selected HIV-related factors that might influence risk of coinfection with leptospirosis, such as degree of immunosuppression, as assessed by CD4 cell count, World Health Organization (WHO) HIV/AIDS clinical stage and antiretroviral therapy (ART) intake. Methods: This was a descriptive cross-sectional analysis of 157 PWH, aged over 18 years old, admitted to the Maputo Central Hospital, in Maputo, Mozambique, between March 2020 and October 2021. The study participants were recruited as a convenience sample regardless of the reasons for their admission. We collected sociodemographic and clinical data, including ART and WHO HIV/AIDS clinical stage, and blood for CD4 cell count and detection of Leptospira IgG antibodies using a commercial Kit ab247199 Leptospira IgG ELISA (www.abcam.com/ab247199) with sensitivity and specificity of 100% and 97.3%, respectively. Laboratory testing was performed at the Faculty of Medicine, Eduardo Mondlane University and Laboratory of Clinical Analysis, in Maputo. Results: Participants were aged 18 to 72 years (median age 39 years; SD ± 10.5), the majority were female 100 (63.7%), from urban areas 138 (87.9%), with secondary-level education 80 (51%). The overall seroprevalence of Leptospira IgG antibodies was 40.1%. The median CD4 cell count was 385 cells/µl (02 to 2297; SD ± 378.47). Higher seroprevalence of Leptospira antibodies was found among participants with CD4 cell counts <250 cells/µl (54.8%), WHO HIV/AIDS stage IV (70.2%) and those on ART (92%), though there were no statistically significant differences between groups with and without Leptospira antibodies. Conclusion: Our study confirmed that Leptospira antibodies are highly prevalent in PWH in Maputo; however, Leptospira infection was not associated with the degree of immunosuppression, WHO HIV/AIDS clinical stage, or the use of ART. Our data support the need for routine screening for leptospirosis in PWH in Mozambique. Future studies are warranted to characterize the incidence and outcomes of symptomatic leptospirosis in this patient population and to identify circulating serovars and species in the country and region, as well as the implicated reservoirs.
RESUMEN
Background: In Mozambique, vegetables are the main source of nutrients and they are consumed raw or cooked. Their consumption, especially in their raw form, might be a source of contamination, which may take place during production and/or transportation as well as in the markets. In this study we aimed to assess the profile and frequency of parasites in lettuces and cabbages sold in some selected markets in Maputo city, the capital of Mozambique. Methods: A cross sectional study was conducted between February and March 2018, in 10 selected markets from Maputo City. A total of 100 combined samples of lettuces and cabbages were analyzed using a spontaneous sedimentation method. Data such as gender and age of the vendors and about handling of vegetables at the point of sale were also collected. Results: Out of the samples, 84 (84%) were contaminated with parasites (86% of lettuces and 82% of cabbages). The analyses performed revealed the presence of E. coli (66%), S. stercoralis (40%), E. histolytica (20%), A. duodenalis (6%), S. haematobium (4%), A. lumbricoides (2%), S. mansoni (2%) and T. trichiura (1%). Two markets, Janete and Fajardo showed contamination in all samples. A Chi square analysis revealed a significant association between the contaminated cabbages and the market where they were obtained. The majority of vendors (57%) do not wash their hands before handling vegetables. Most of the vendors had tables to place vegetables (72%) and those tables are cleaned before display (86%). None of the differences observed in each risk factor studied were statistically significant. Conclusion: The present study highlights that contamination of raw vegetables with pathogenic parasites in markets might represent a vector for transmission of intestinal and water- borne parasites to consumers. Future studies should investigate the extent of vegetable contamination at each stage of the supply chain from the farm to consumers, as well as its relationship to human parasitic infection and its consequences. Good hygienic practices of farmers, vendors and consumers should be enforced to break the contamination chain. Whenever possible, hydroponic cultivation should be encouraged to avoid the influence of highly contaminated soils.
