Intuitive eating has reduced the chances of being overweight in university students during the COVID-19 pandemic.

Introduction: University students have been particularly affected during the COVID-19 pandemic, and several sociodemographic and behavioral factors may be associated with the risk of overweight in this population. The aim of this study was to assess the impact of the pandemic on the eating behavior and nutritional status of university students, and the factors associated with these changes, especially the role of intuitive eating in this process. Methods: This is a repeated measures observational study with data collected in the first and third year of the COVID-19 pandemic in Brazil, from students over the age of 18 in the undergraduate programs of a federal university in southeastern Brazil. The survey was conducted using an online form. Eating behavior was assessed using the "Intuitive Eating Scale-2". Results: 251 university students took part, most of them female with a median age of 22. There was an increase in body mass index (BMI) and intuitive eating score between the two periods. In the first year of the pandemic, being female and using tobacco reduced the chances of students being overweight. Living in a marital relationship, worrying about weight gain and body dissatisfaction increased the chances of this outcome. In the third year of the pandemic, it was observed that practicing restrictive diets, having inadequate body perception, worrying about weight gain and living in a marital relationship increased the chances of students being overweight. Being female and eating more intuitively, in line with bodily needs, reduced these odds, demonstrating a protective role in this scenario. Conclusion: There was an increase in BMI and intuitive eating score during the pandemic. Sociodemographic, lifestyle and behavioral variables had both a positive and negative influence on nutritional status. Intuitive eating was shown to be a protective factor during this period, reducing the chances of being overweight in this population. Thus, more intuitive eating may favor greater weight stability, and may, therefore, have helped to reduce the impact of the pandemic on weight gain. In this way, people who ate more intuitively partially resisted the context that favored weight gain (stress, changes in diet and physical inactivity).

Intervenção Educativa Sobre Dermatite Associada à Incontinência: Estratégia de Ensino no Instagram / Educational Intervention on Incontinence-associated Dermatitis: Teaching Strategy on Instagram / Intervención Educativa en Dermatitis Asociada a Incontinencia: Estrategia Didáctica Sobre Instagram

Objetivos:Descrever e avaliar intervenção educativa realizada por meio de mídia social sobre dermatite associada à incontinência (DAI) com profissionais de enfermagem. Métodos:Estudo de intervenção educativa com abordagem qualitativa pela mídia social Instagram para veiculação dos conteúdos do curso sobre a DAI. A coleta de dados foi realizada com 30 profissionais de enfermagem da clínica médica e unidade de terapia intensiva adulta de hospital de ensino público de Mato Grosso do Sul, de fevereiro a março de 2022. Os dados foram coletados durante o curso e apreciados pela análise de conteúdo de Bardin e à luz do referencial teórico da aprendizagem significativa de Ausubel. Resultados: Da análise dos comentários dos cursistas, emergiram quatro categorias temáticas: ancoragem, que retrata o saber prévio; aprendizagem significativa subordinada, a qual apresenta a construção de conhecimento ancorada no saber prévio; aprendizagem por descoberta, evidenciando a autonomia do participante no aprendizado; e avaliação do processo de ensino-aprendizagem, que revela a avaliação do processo educativo. Conclusão: Os resultados mostraram que a intervenção educativa obteve êxito no que tange ao compartilhamento de evidências sobre DAI aos participantes e que a ferramenta Instagram pode ser adotada para ações educativas mesmo em ambiente hospitalar.

Objectives:To describe and evaluate, in a formative way, an educational intervention carried out through social media on incontinence-associated dermatitis with nursing professionals from a public hospital. Method: Educational intervention study with a qualitative approach conducted in a virtual way through the social media Instagram as a platform for the dissemination of course content on the subject. Data collection was carried out with 30 nursing professionals from the medical clinic and adult intensive care unit of a public teaching hospital in the state of Mato Grosso do Sul, Brazil, between February and March 2022. Data were collected during the course and analysed through content analysis and according to Ausubel's meaningful learning theoretical framework perspective. Results: From the analysis of the participants' comments, four thematic categories emerged: anchoring; subordinated meaningful learning; discovery learning; and evaluation of the teaching-learning process. Conclusion: Through the results found, it was identified that the educational intervention conducted was successful in terms of sharing evidence on the subject to the participants. It was found that the Instagram can be adopted as a tool to carry out educational actions, including in a hospital environment.

Objetivo:Describir y evaluar, de forma formativa, una intervención educativa realizada a través de las redes sociales sobre dermatitis asociada a incontinencia con profesionales de enfermería de un hospital público. Métodos: Estudio de intervención educativa, con enfoque cualitativo, realizado de forma virtual, a través de la red social Instagram® como plataforma de difusión de contenidos de cursos sobre el tema. La recolección de datos se realizó con 30 profesionales de Enfermería de la Clínica Médica y UTI Adultos de un hospital público de enseñanza en el estado de Mato Grosso do Sul, entre febrero y marzo de 2022. Este estudio fue aprobado por el Comité de Ética en Investigación. Los datos fueron recolectados durante el curso y analizados a través del análisis de contenido ya la luz del marco teórico del Aprendizaje Significativo de Ausubel. Resultados: Del análisis de los comentarios de los participantes surgieron cuatro categorías temáticas: anclaje; aprendizaje significativo subordinado; aprendizaje por descubrimiento; y evaluación del proceso de enseñanza-aprendizaje. Conclusión: A través de los resultados encontrados, se identifica que la intervención educativa realizada fue exitosa en cuanto a compartir evidencias sobre el tema a los participantes. Se constató que la herramienta Instagram® puede ser adoptada para realizar acciones educativas, incluso en ambiente hospitalario.

Piperazine designer drugs elicit toxicity in the alternative in vivo model Caenorhabditis elegans.

Piperazine designer drugs are a group of synthetic drugs of abuse that have appeared on the illicit market since the second half of the 1990s. The most common derivatives are 1-benzylpiperazine (BZP), 1-(4-methoxyphenyl)piperazine (MeOPP) and 1-(3,4-methylenedioxybenzyl)piperazine (MDBP). They can be consumed as capsules, tablets, but also in powder or liquid forms. Generally, although less potent than amphetamines, piperazines have dopaminergic and serotonergic activities. The aim of this work was to evaluate the toxic effects of BZP, MeOPP and MDBP using Caenorhabditis elegans as in vivo model for acute toxicity, development, reproduction and behavior testing. The LC50 for BZP, MeOPP and MDBP were 52.21, 5.72 and 1.22 mm, respectively. All concentrations induced a significant decrease in the body surface of the worms, indicating developmental alterations, and decrease in the brood size. Worms exposed to piperazine designer drugs also presented a decrease in locomotor activity and mechanical sensitivity, suggesting the possible dysfunction of the nervous system. Neuronal damage was confirmed through the decrease in fluorescence of BY200 strains, indicating loss of dopaminergic transporters. In conclusion, we suggest that piperazine designer drugs lead to neuronal damage, which might be the underlying cause of the altered behavior observed in humans.

Depressive symptoms and associated factors in elderly long-term care residents. / Sintomas depressivos e fatores associados em idosos residentes em instituição de longa permanência.

OBJECTIVE: To determine the prevalence and factors associated with depressive symptoms in institutionalized elderly. METHODS: This is an epidemiological cross-sectional study with 42 elderly in a Long-Term Care Institution for the Elderly (LTCIE). Data was collected from April to December 2014 through a questionnaire with information on demographic and socioeconomic aspects, the Geriatric Depression Scale short version (GDS-15) and the Mini Mental State Examination (MMSE). RESULTS: Of the elderly studied, 54.8% had depressive symptoms and were predominantly females (64.7%). There was a significant association between depressive symptoms and variables retired (p = 0.043); urinary incontinence (p = 0.028); self-perceived health (p-value = 0.042) and sleep quality (p-value = 0.000). CONCLUSION: The study found a high prevalence of depressive symptoms in institutionalized elderly, associated with the presence of urinary incontinence, (negative) self-perceived health, (poor) quality of sleep and retirement (yes). Following the study and in the face of the needs of this population, it is necessary to seek measures that act directly on the modifiable variables, preventing and treating them.

O objetivo deste artigo é verificar a prevalência e fatores associados a sintomas depressivos em idosos institucionalizados. Trata-se de um estudo epidemiológico com delineamento transversal, composto por 42 idosos de uma Instituição de Longa Permanência para Idosos (ILPI). A coleta de dados foi realizada no período de abril a dezembro de 2014 por meio de um questionário com informações sobre aspectos demográficos e socioeconômicos, a Escala de Depressão Geriátrica em versão reduzida (EDG-15) e o Mini Exame do Estado Mental (MEEM). Dos idosos estudados, 54,8% apresentaram sintomas depressivos, predominando o sexo feminino com 64,7%. Houve associação significativa entre sintomas depressivos e as variáveis: aposentado (p = 0,043); incontinência urinária (p = 0,028); autopercepção de saúde (p-valor = 0,042) e qualidade do sono (p-valor = 0,000). O estudo verificou alta prevalência de sintomas depressivos em idosos institucionalizados, associado às variáveis presença de incontinência urinária, autopercepção de saúde (negativa), qualidade de sono (ruim) e aposentadoria (sim). Através do estudo e diante das necessidades enfrentadas por essa população, faz-se necessário a busca por medidas que atuem diretamente nas variáveis modificáveis, prevenindo e tratando-as.

Sintomas depressivos e fatores associados em idosos residentes em instituição de longa permanência / Depressive symptoms and associated factors in elderly long-term care residents

Resumo O objetivo deste artigo é verificar a prevalência e fatores associados a sintomas depressivos em idosos institucionalizados. Trata-se de um estudo epidemiológico com delineamento transversal, composto por 42 idosos de uma Instituição de Longa Permanência para Idosos (ILPI). A coleta de dados foi realizada no período de abril a dezembro de 2014 por meio de um questionário com informações sobre aspectos demográficos e socioeconômicos, a Escala de Depressão Geriátrica em versão reduzida (EDG-15) e o Mini Exame do Estado Mental (MEEM). Dos idosos estudados, 54,8% apresentaram sintomas depressivos, predominando o sexo feminino com 64,7%. Houve associação significativa entre sintomas depressivos e as variáveis: aposentado (p = 0,043); incontinência urinária (p = 0,028); autopercepção de saúde (p-valor = 0,042) e qualidade do sono (p-valor = 0,000). O estudo verificou alta prevalência de sintomas depressivos em idosos institucionalizados, associado às variáveis presença de incontinência urinária, autopercepção de saúde (negativa), qualidade de sono (ruim) e aposentadoria (sim). Através do estudo e diante das necessidades enfrentadas por essa população, faz-se necessário a busca por medidas que atuem diretamente nas variáveis modificáveis, prevenindo e tratando-as.

Abstract Objective: To determine the prevalence and factors associated with depressive symptoms in institutionalized elderly. Methods: This is an epidemiological cross-sectional study with 42 elderly in a Long-Term Care Institution for the Elderly (LTCIE). Data was collected from April to December 2014 through a questionnaire with information on demographic and socioeconomic aspects, the Geriatric Depression Scale short version (GDS-15) and the Mini Mental State Examination (MMSE). Results: Of the elderly studied, 54.8% had depressive symptoms and were predominantly females (64.7%). There was a significant association between depressive symptoms and variables retired (p = 0.043); urinary incontinence (p = 0.028); self-perceived health (p-value = 0.042) and sleep quality (p-value = 0.000). Conclusion: The study found a high prevalence of depressive symptoms in institutionalized elderly, associated with the presence of urinary incontinence, (negative) self-perceived health, (poor) quality of sleep and retirement (yes). Following the study and in the face of the needs of this population, it is necessary to seek measures that act directly on the modifiable variables, preventing and treating them.

Microcystin-LR exposure induces oxidative damage in Caenorhabditis elegans: Protective effect of lutein extracted from marigold flowers.

Microcystin-LR (MIC-LR) is a hepatotoxin, with toxicity mechanisms linked to oxidative stress. Besides, neurotoxic effects of MIC-LR have recently been described. Herein, we evaluated the effects of environmentally important concentrations of MIC-LR (1, 10, 100, 250, and 500 µg/L) on oxidative stress markers and the survival rate of the nematode Caenorhabditis elegans (C. elegans). In addition, a possible protective effect of the carotenoid lutein (LUT) extracted from marigold flowers against MIC-LR toxicity was investigated. Higher concentrations (250 and 500 µg/L) of MIC-LR induced the generation of reactive oxygen species (ROS) and resulted in a survival loss in C elegans. Meanwhile, all MIC-LR concentrations caused an increase in the superoxide dismutase (SOD) expression, while catalase (CAT) expression was only affected at 500 µg/L. The carotenoid LUT prevented the ROS generation, impairment in the CAT expression, and the survival loss induced by MIC-LR in C. elegans. Our results confirm the toxicity of MIC-LR even in a liver-lacking invertebrate and the involvement of oxidative events in this response. Additionally, LUT appears to be able to mitigate the MIC-LR toxic effects.

Development of an application for mobile devices to identify the frailty phenotype among the elderly / Desenvolvimento de aplicativo para dispositivos móveis voltado para identificação do fenótipo de fragilidade em idosos

Abstract Objective: to develop a mobile app to quickly and safely identify frailty syndrome features among the elderly. Method: a cross-sectional study was conducted. The application was developed for the Android platform in the Java programming language and XML markup. The study instrument was based on five frailty phenotype criteria. The tests were conducted with 20 elderly persons living in a long-term care facility. Results: the twenty elderly persons had a mean age of 76.55 (±9.5) years. Thirteen were identified as frail, five were pre-frail and two were non-frail. The comparison of the results of the instruments of analysis coincided in the general evaluation of frailty and in the individual identification of the five criteria. Conclusion: the data suggests that the use of the application for the evaluation of frailty among the elderly was performed safely, with the advantage of quick access to allow the monitoring of the clinical status and prognosis of the patient.

Resumo Objetivo: desenvolver um aplicativo para dispositivos móveis com o intuito de identificar de maneira rápida e segura as características da síndrome de fragilidade em idosos. Método: estudo transversal quantitativo. O aplicativo foi desenvolvido para a plataforma Android, em linguagem de programação Java e marcação em XML. Os fundamentos utilizados foram baseados nos cinco parâmetros para definição do fenótipo de fragilidade. Os testes foram realizados com 20 idosos residentes em uma instituição de longa permanência. Resultados: a média de idade foi de 76,55 (±9,5) anos. Dentre os 20 idosos investigados, 13 foram classificados como frágeis, cinco pré-frágeis e dois não frágeis. A comparação dos resultados entre os instrumentos de análise coincidiram na avaliação geral da fragilidade e na identificação dos cinco critérios individualmente. Conclusão: a utilização do aplicativo para avaliação da fragilidade em idosos foi feita de maneira segura, com a vantagem do acesso rápido às informações para acompanhamento do quadro clínico e prognóstico do paciente.

DNA damage in the elderly is associated with 5-MTHF levels: a pro-oxidant activity.

The aging phenomenon is associated with oxidative stress damage in biomolecules, especially DNA. 5-Methyltetrahydrofolate (5-MTHF), the active folate form, plays a pivotal role in maintaining genomic integrity. However, recently it was associated with cancer development. In Brazil, there are folic acid enriched foods, such as flour, making the general population chronically exposed to folates. Therefore, the aim of this study was to investigate whether erythrocytes 5-MTHF levels were associated with age-related DNA damage in two groups (elderly and young subjects). Additionally, a study in Caenorhabditis elegans, an in vivo alternative model, was performed to verify if 5-MTHF presents a pro-oxidant effect. A total of 50 elderly and 25 young subjects participated in this study, which analyzed whole blood DNA damage, plasma carbonyl proteins (PCO), and erythrocytes 5-MTHF levels. In addition, ROS and RNS production, survival rate, and lifespan were performed in C. elegans exposed to 5-MTHF. Blood 5-MTHF levels and DNA damage were increased in the elderly compared to the young group. A positive association was found between 5-MTHF levels and DNA damage, and between DNA damage and PCO levels, suggesting an oxidative cause of damage associated with the active folate form. In an experimental study it was observed that 5-MTHF increased ROS production in C. elegans, in a dose dependent manner, while survival rate and life span were not affected at the test doses. These findings suggest that 5-MTHF, the active folate form, may be involved in DNA damage in the elderly. This damage could be a result of oxidative stress, as observed in the in vivo alternative model; however, more studies are necessary to prove our present results.

Association between inflammation processes, DNA damage, and exposure to environmental pollutants.

Environmental exposure to pollutants, especially polycyclic aromatic hydrocarbons (PAHs), could lead to carcinogenesis development. However, there is a gap on the mechanisms involved in this effect. Therefore, the aim of this study was to investigate the potential relationship between exposure to environmental air pollution and inflammation process in DNA damage in taxi drivers. This study included 45 taxi drivers and 40 controls; non-smokers composed both groups. Biological monitoring was performed through quantification of urinary 1-hydroxypyrene (1-OHP). ICAM-1 (CD54) expression, NTPDase activity, inflammatory cytokine (IL-1ß, IL-6, IL-10, TNF-α and IFN-γ) levels, and comet and micronucleus assays were evaluated. The results demonstrated that 1-OHP levels, ICAM-1 expression, NTPDase activity, and DNA damage biomarkers (% tail DNA and micronucleus frequency) were increased in taxi drivers compared to the control group (p < 0.01). Moreover, significant associations were found between 1-OHP levels and ICAM-1 expression, % tail DNA, and micronucleus frequency (p < 0.05). Besides, pro-inflammatory cytokine levels were positively correlated to % tail DNA and micronucleus frequency (p < 0.001). Our findings suggest an important association between environmental exposure to air pollution with increase of ICAM-1 expression and NTPDase activity in taxi drivers. Additionally, the multiple regression linear-analysis demonstrated association between IL-6 and DNA damage. Thus, the present study has provided important evidence that, in addition to environmental exposure to air pollutants, the inflammation process may contribute to DNA damage.

Profile of work-related road traffic accident victims recorded by sentinel health units in Pernambuco, Brazil, 2012-2014. / Perfil das vítimas de acidentes de transporte terrestre relacionados ao trabalho em unidades de saúde sentinelas de Pernambuco, 2012 - 2014.

OBJECTIVE: to describe the profile of work-related road traffic accident (RTA) victims, reported by Road Traffic Accident Information Sentinel Units in the state of Pernambuco, Brazil, from 2012-2014. METHODS: descriptive study of fatal and non-fatal work-related road traffic accident victims, reported by 21 RTA Information Sentinel Units in Pernambuco. RESULTS: 87.8% of the 10,691 cases reported occurred among males; 69.0% of all records were related to the 20-39 age group; the sectors with most injured workers were Transport (24.4%) and Trade (21.3%); most of the victims were drivers (82.0%) and motorcycles were the most frequent vehicle at the time of the accident (77.0%). CONCLUSION: victims were predominantly young male motorcyclists; findings may serve to inform intersectoral actions to prevent work-related RTAs, appropriate to the profile of the victims.

Perfil das vítimas de acidentes de transporte terrestre relacionados ao trabalho em unidades de saúde sentinelas de Pernambuco, 2012 - 2014 / Perfil de las víctimas de accidentes de tránsito relacionados con el trabajo en unidades de salud centinela de Pernambuco, Brasil, en el período de 2012 a 2014 / Profile of work-related road traffic accident victims recorded by sentinel health units in Pernambuco, Brazil, 2012-2014

OBJETIVO: descrever o perfil das vítimas de acidentes de transporte terrestre (ATT) relacionados ao trabalho, notificados nas Unidades Sentinelas de Informação sobre Acidentes de Transporte Terrestre no estado de Pernambuco, Brasil, no período 2012-2014. MÉTODOS: estudo descritivo das vítimas (fatais e não fatais) de acidente de transporte terrestre relacionados ao trabalho, notificados em 21 Unidades Sentinelas de Informação sobre ATT de Pernambuco. RESULTADOS: dos 10.691 casos notificados, 87,8% ocorreram entre homens; do total dos registros, 69,0% concentraram-se na faixa etária de 20 a 39 anos; os setores com mais trabalhadores acidentados foram Transporte (24,4%) e Comércio (21,3%); a maioria das vítimas eram condutores (82,0%) e a motocicleta o meio de locomoção mais utilizado no momento do acidente (77,0%). CONCLUSÃO: entre as vítimas, predominaram motociclistas jovens e do sexo masculino; os achados poderão subsidiar ações intersetoriais de prevenção dos ATT relacionados ao trabalho, adequadas ao perfil das vítimas.

OBJETIVO: describir el perfil de las víctimas de accidentes de tránsito (ATT) relacionados con el trabajo, notificados en Unidades Centinelas de Información sobre Accidentes de Transporte Terrestre en el estado de Pernambuco, Brasil, en el período 2012-2014. MÉTODOS: estudio descriptivo de las víctimas (mortal y no mortal) de ATT relacionados con el trabajo, notificados en 21 Unidades Centinelas de Información sobre ATT de Pernambuco. RESULTADOS: de los 10.691 casos registrados, 87,8% ocurrieron en hombres; 69,0% de los registros se centraron en el grupo de edad de 20-39 años; los sectores con más trabajadores accidentados fueron Transporte (24,4%) y Comercio (21,3%); la mayoría de las víctimas eran conductores (82,0%) y la motocicleta el medio de locomoción más usado en el momento del accidente (77,0%). CONCLUSIÓN: entre las víctimas, predominaron ciclistas jóvenes y hombres; los hallazgos pueden subsidiar acciones intersectoriales para prevenir los ATT relacionados con el trabajo, adecuadas al perfil de las víctimas.

OBJECTIVE: to describe the profile of work-related road traffic accident (RTA) victims, reported by Road Traffic Accident Information Sentinel Units in the state of Pernambuco, Brazil, from 2012-2014. METHODS: descriptive study of fatal and non-fatal work-related road traffic accident victims, reported by 21 RTA Information Sentinel Units in Pernambuco. RESULTS: 87.8% of the 10,691 cases reported occurred among males; 69.0% of all records were related to the 20-39 age group; the sectors with most injured workers were Transport (24.4%) and Trade (21.3%); most of the victims were drivers (82.0%) and motorcycles were the most frequent vehicle at the time of the accident (77.0%). CONCLUSION: victims were predominantly young male motorcyclists; findings may serve to inform intersectoral actions to prevent work-related RTAs, appropriate to the profile of the victims.

Caenorhabditis elegans as an alternative in vivo model to determine oral uptake, nanotoxicity, and efficacy of melatonin-loaded lipid-core nanocapsules on paraquat damage.

Caenorhabditis elegans is an alternative in vivo model that is being successfully used to assess the pharmacological and toxic effects of drugs. The exponential growth of nanotechnology requires the use of alternative in vivo models to assess the toxic effects of theses nanomaterials. The use of polymeric nanocapsules has shown promising results for drug delivery. Moreover, these formulations have not been used in cases of intoxication, such as in treatment of paraquat (PQ) poisoning. Thus, the use of drugs with properties improved by nanotechnology is a promising approach to overcome the toxic effects of PQ. This research aimed to evaluate the absorption of rhodamine B-labeled melatonin (Mel)-loaded lipid-core nanocapsules (LNC) by C. elegans, the application of this model in nanotoxicology, and the protection of Mel-LNC against PQ damage. The formulations were prepared by self-assembly and characterized by particle sizing, zeta potential, drug content, and encapsulation efficiency. The results demonstrated that the formulations had narrow size distributions. Rhodamine B-labeled Mel-LNC were orally absorbed and distributed in the worms. The toxicity assessment of LNC showed a lethal dose 50% near the highest dose tested, indicating low toxicity of the nanocapsules. Moreover, pretreatment with Mel-LNC significantly increased the survival rate, reduced the reactive oxygen species, and maintained the development in C. elegans exposed to PQ compared to those worms that were either untreated or pretreated with free Mel. These results demonstrated for the first time the uptake and distribution of Mel-LNC by a nematode, and indicate that while LNC is not toxic, Mel-LNC prevents the effects of PQ poisoning. Thus, C. elegans may be an interesting alternative model to test the nanocapsules toxicity and efficacy.

Percepção de enfermeiros sobre os pais de prematuros em unidade de terapia intensiva neonatal / Nurses’ perception on parents of premature babies in the neonatal intensive care unit

O estudo objetivou analisar a percepção dos enfermeiros sobre os pais durante o tratamento na UTI Neonatal. Pesquisa qualitativa, realizada de março a junho de 2012 por entrevista semiestrututrada com nove enfermeiros de um hospital em Recife-PE. Os dados mostram que os pais são, inicialmente, percebidos em estado de desorganização emocional, mas, quando eles recorrem a estratégias defensivas e com o auxílio dos enfermeiros, os quais propiciam informações clínicas e apoio psicológico, eles se ajustam à situação do tratamento. Por outro lado, a comunicação do óbito do prematuro é uma vivência percebida como extremamente negativa. Para melhor efetivar a humanização da assistência, os enfermeiros salientam a necessidade da abordagem interdisciplinar.