RESUMEN
What we generally refer to as 'vitamin A' is a group of naturally-occurring molecules structurally similar to retinol that are capable of exerting biological activity. These retinoids are essential to diverse physiological functions including vision, immune response, bone mineralization, reproduction, cell differentiation, and growth. As well, some retinoids have antioxidant properties. Independent studies published over the last few decades have revealed that many fish and wildlife populations living in highly polluted environments have altered retinoid status possibly associated with retinoid metabolic or homeostatic mechanisms. Substantial evidence links organic contaminant exposure with changes in retinoid status in animal populations, but only a few detailed studies have been published implicating inorganic contaminants such as metals. This mini-review selectively deals with field and laboratory studies reporting associations between environmental contaminants, especially trace metals, and alterations in retinoid status. Both essential and non-essential trace metals have been reported to affect retinoid status. This review focuses on metabolic imbalances of retinoids in relation to metal contamination and illustrates possible modes of action. The role of retinoids as antioxidants and their potential as biomarkers of metal contamination are discussed.
Asunto(s)
Antioxidantes/metabolismo , Contaminantes Ambientales/efectos adversos , Metales/efectos adversos , Retinoides/metabolismo , Animales , Antioxidantes/efectos adversos , Biomarcadores/metabolismo , Aves , Ecosistema , Exposición a Riesgos Ambientales/efectos adversos , Monitoreo del Ambiente/métodos , Peces , Hipervitaminosis A/metabolismo , Mamíferos , Retinoides/efectos adversos , Medición de RiesgoRESUMEN
Hexanitrohexaazaisowurtzitane, or CL-20, is an emerging highly energetic compound currently under consideration for military applications. With the anticipated wide use of CL-20, there is the potential for soil and groundwater contamination resulting in adverse toxicologic effects on environmental receptors. Presently, there is a lack of data describing the toxic effects of CL-20 on avian species. The present study describes the effect of CL-20 on Japanese quail (Coturnix coturnix japonica) modified from standard toxicity test guidelines. First, a 14-day subacute assay was adopted using repeated gavage doses (0, 307, 964, 2439, 3475, or 5304 mg CL-20/kg body weight (BW)/d for 5 days followed by no CL-20 exposure (vehicle only) for 10 days. Second, a subchronic feeding assay (0, 11, 114, or 1085 mg CL-20/kg feed) was done for 42 days. During both studies, no overt toxicity was observed in the CL-20-treated birds. During the first 5 days of the subacute study, CL-20-exposed birds showed a dose-dependent decrease in BW gain, whereas increased liver weight, plasma sodium, and creatinine levels were observed in birds receiving the highest dose tested. For the subchronic study, embryo weights were significantly decreased in a dose-dependent manner. Embryos from CL-20-exposed birds were observed to have multiple cranial and facial deformities, beak curvatures, possible mid-brain enlargement, and classic one-sided development with micro-opthalamia (nonstatistical comparisons with control embryos). A trend toward decreased number of eggs laid per female bird was also observed. We conclude that CL-20 (or its degradation products) elicits few effects in adults but may affect avian development, although these preliminary findings should be confirmed.
Asunto(s)
Compuestos Aza/toxicidad , Coturnix/crecimiento & desarrollo , Contaminantes Ambientales/toxicidad , Compuestos Heterocíclicos/toxicidad , Pruebas de Toxicidad/métodos , Administración Oral , Animales , Compuestos Aza/farmacocinética , Peso Corporal/efectos de los fármacos , Anomalías Congénitas/etiología , Coturnix/embriología , Coturnix/metabolismo , Creatinina/sangre , Relación Dosis-Respuesta a Droga , Desarrollo Embrionario/efectos de los fármacos , Contaminantes Ambientales/farmacocinética , Compuestos Heterocíclicos/farmacocinética , Tamaño de los Órganos/efectos de los fármacos , Sodio/sangre , Distribución TisularRESUMEN
Retinoids stored in the avian egg are essential for normal development, however, laboratory and field experiments suggest that they are affected by environmental contaminants. Lecithin:retinol acyltransferase (LRAT) activity was detected in the microsomal fraction of the yolk-sac membrane of the Japanese quail at day 6 of development. LRAT activity was maximal at pH 7.0 having apparent kinetic parameters of K(m)=1.35 microM and V(max)=0.21 nmol/mg protein/h and was inhibited by the sulfhydryl modifying agent N-ethyl-maleimide. Retinol ester hydrolase (REH) activity in the microsomal fraction of the yolk-sac membrane was stimulated by the bile salt analogue 3-[(3-cholamidopropyl) dimethyl-ammonio]-1-propane sulfonate and was maximal at pH 9.0 with apparent K(m)=77 microM and V(max)=34.3 nmol/mg protein/h. Injection of the PCB congener 2,3,3',4,4'-pentachlorobiphenyl increased both REH and LRAT activities, whereas 2,3,3',4-tetrachlorobiphenyl stimulated LRAT. Yolk retinol concentration and the molar ratio retinol:retinyl palmitate were lower in the exposed eggs. Yolk retinol concentration decreased as LRAT increased (R(2)=0.89) suggesting that certain PCB congeners may affect vitamin A mobilization in ovo by increasing LRAT activity in the yolk-sac membrane.
Asunto(s)
Aciltransferasas/metabolismo , Hidrolasas de Éster Carboxílico/metabolismo , Coturnix , Contaminantes Ambientales/toxicidad , Bifenilos Policlorados/toxicidad , Retinoides/metabolismo , Vitamina A/análogos & derivados , Saco Vitelino/efectos de los fármacos , Saco Vitelino/enzimología , Animales , Diterpenos , Relación Dosis-Respuesta a Droga , Yema de Huevo/metabolismo , Inhibidores Enzimáticos/farmacología , Etilmaleimida/farmacología , Microsomas/efectos de los fármacos , Microsomas/enzimología , Ésteres de Retinilo , Vitamina A/metabolismoRESUMEN
Recent reports of extremely low retinoid stores in fish living in contaminated river systems prompted an initial investigation of the mechanisms of hepatic storage and mobilization in brook trout. Enzyme characterization in microsomes revealed a lecithin:retinol acyltransferase activity (LRAT) optimum in the alkaline range (pH 9.0; Vmax = 0.6 nmol per mg prot. h(-1); Km = 10.2 microM) which is not known to occur in mammals, in addition to a secondary optimum at pH 6.5 typical of mammals. Acyl CoA:retinol acyltransferase (ARAT) kinetic parameters were quite different to those of mammals. The substrate affinity of trout ARAT (Km = 1.6 microM) was approximately 22-fold greater than that of the rat while maximal velocity (Vmax = 0.2 nmol per mg prot. h(-1)) was 18-fold less. Retinyl ester hydrolase activity (REH) was optimal under acid conditions (pH 4.2; Vmax = 6.6 nmol per mg prot. h(-1); Km = 0.6 mM), was inhibited by a bile salt analogue and was greater in males than females. This REH was tentatively categorized as a bile salt-independent, acid retinyl ester hydrolase (BSI-AREH). REH was inhibited in a dose-dependent manner following in vivo exposure to a representative environmental contaminant the coplanar polychlorinated biphenyl (PCB), 3,3',4,4'-tetrachlorobiphenyl (TCBP). Inhibition may be an indirect effect because enzyme activity was not affected by in vitro exposure of control microsomes. REH inhibition in the brook trout may affect the uptake of retinyl esters (REs) from chylomicron remnants as well as the mobilization of stored REs.
Asunto(s)
Hígado/efectos de los fármacos , Bifenilos Policlorados/farmacología , Trucha/metabolismo , Vitamina A/metabolismo , Aciltransferasas/antagonistas & inhibidores , Aciltransferasas/metabolismo , Animales , Hidrolasas de Éster Carboxílico/antagonistas & inhibidores , Hidrolasas de Éster Carboxílico/metabolismo , Inhibidores Enzimáticos/farmacología , Esterificación , Femenino , Hidrólisis , Hígado/enzimología , Hígado/metabolismo , MasculinoRESUMEN
Male and female Sprague-Dawley rats were fed diets incorporating lyophilized chinook salmon obtained from Lake Ontario and Lake Huron. After 70 days, females were bred and the progeny (F1) were reared on the same fish-based diets as the adults (F0). After 78-133 days on the diets, males and females of both generations were sacrificed and hepatic microsomal enzyme activities determined, along with glutathione S-transferase-placental form (GSTP) expression and hepatic cellular proliferation. Hepatic P450 enzyme activities (MROD, EROD, PROD, BROD, and aminopyrine) were increased significantly by fish diets from both sources. Increases in hepatic enzyme activity were greatest for fish caught from Lake Ontario and reflected the total levels of organochlorine contaminants in the fish. GSTP and cell proliferation rates did not show any diet-related or dose-related changes. Vitamin A stores were analyzed as the concentration of liver retinyl palmitate. In rats receiving the highest TEQ dose (i.e., 20% Lake Ontario fish diet), vitamin A stores were significantly lower in F0 adults, F1 weanlings, and F1 adult females.
Asunto(s)
Alimentación Animal/toxicidad , Contaminación de Alimentos , Glutatión Transferasa/efectos de los fármacos , Hígado/efectos de los fármacos , Microsomas Hepáticos/efectos de los fármacos , Salmón , Contaminantes Químicos del Agua/toxicidad , Animales , División Celular/efectos de los fármacos , Efecto de Cohortes , Femenino , Glutatión Transferasa/metabolismo , Hígado/enzimología , Hígado/fisiología , Masculino , Microsomas Hepáticos/enzimología , Microsomas Hepáticos/metabolismo , Placenta/efectos de los fármacos , Placenta/enzimología , Antígeno Nuclear de Célula en Proliferación/análisis , Antígeno Nuclear de Célula en Proliferación/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Vitamina A/fisiologíaRESUMEN
Male Wistar rats were injected ip with 0 or 20 mg/kg 3,3',4,4',5,5'-hexabromobiphenyl and blood samples were collected 1, 3, 6, 7, and 8 d later. At 8 d after the injection, serum retinol was increased 30% and serum thyroxine was decreased 26% relative to control values. These effects were apparently unrelated to transthyretin in that the biphenyl did not alter the proportion of thyroxine binding in vitro to the prealbumin fraction of serum proteins. Separate groups of control and HBBP-injected rats did not receive food on d 7 (i.e., 24-h fast) and d 8 after injection (i.e., 48-h fast). Fasting decreased the serum retinol and thyroxine concentrations as well as the proportion of thyroxine binding in vitro to the prealbumin fraction of serum. The decreases in retinol and thyroxine concentrations associated with fasting are therefore ascribed to a decrease in the concentration of transthyretin in circulation.
Asunto(s)
Ayuno/metabolismo , Bifenilos Polibrominados/farmacología , Tiroxina/sangre , Vitamina A/sangre , Animales , Transporte Biológico , Cromatografía Líquida de Alta Presión , Electroforesis en Gel de Poliacrilamida , Inyecciones Intraperitoneales , Masculino , Bifenilos Polibrominados/administración & dosificación , Prealbúmina/metabolismo , Ratas , Ratas Wistar , Tiroxina/metabolismo , Proteínas de Unión a Tiroxina/metabolismoRESUMEN
: The potential use of retinoids and ß-carotene as biomarkers in the eggs of the Great Blue Heron was investigated. In the spring of 1991, 65 eggs were collected from nine heronries (seven along the St Lawrence River and two reference sites). A method was specifically developed for the extraction and analysis of ß-carotene and the retinoids in heron egg yolks by reversed-phase HPLC. When results were expressed either as the molar ratio of retinol: retinyl palmitate or as retinyl palmitate concentration, significant differences were found between colonies; however, retinyl palmitate concentration was deemed the better biomarker because it was not significantly influenced by embryonic stage of development. Retinyl palmitate concentrations in freshwater colonies were negatively related to PCB congeners Nos 105 and 118 as well as their TCDD-EQ values (p < 0.02, r (2)=0.78). Egg tetrachloro-mono-ortho biphenyl concentrations were also negatively related to retinyl palmitate (p < 0.005, r (2)=0.90). With the exception of the two mono-ortho co-planar congeners detected in the present study, the contamination levels found in heron eggs were well below those found for other bird species in the Great Lakes area and, so far, no detrimental effects have been reported in Great Blue Heron populations in Quebec. These results suggest that retinyl palmitate may be useful as a sensitive and non-invasive biomarker for monitoring organochlorine contaminant effects in the Great Blue Heron in freshwater sites.
RESUMEN
Juvenile male Wistar rats were injected i.p. with 0, 20, or 40 mg/kg 3,3',4,4',5,5'-hexabromobiphenyl and blood samples collected periodically up to 28 days. A dose-dependent depression of the serum thyroxine level was detected, while the circulating triiodothyronine concentration was not affected by the biphenyl congener. Thyroxine turnover in vivo 7 days after injection of the 20 mg/kg dose revealed significant increases of various clearance parameters relative to controls. The fractional clearance rate (day-1) increased by 84%, the daily metabolic clearance rate (mL.kg-1.day-1) increased by 128%, and the daily thyroxine disposal rate (ng.kg-1.day-1) increased by 41%. Also, the thyroxine distribution space (mL/kg) increased by 21%. These results indicated greater thyroxine binding in major organs as well as a marked increase in the peripheral metabolism of thyroxine. The increased thyroxine metabolism is explained by a 4.8-fold induction of uridine 5'-diphosphoglucuronyltransferase activity in liver microsomes. The type I 5'-deiodinase activity in liver homogenates and endogenous concentrations of the cofactor for this reaction, glutathione, were not affected by the biphenyl. This result means that homeostatic mechanisms involving thyroxine conversion to triiodothyronine do not explain the maintenance of serum T3 under these conditions.
Asunto(s)
Bifenilos Polibrominados/farmacología , Tiroxina/metabolismo , Triyodotironina/biosíntesis , Animales , Colesterol/sangre , Relación Dosis-Respuesta a Droga , Ingestión de Alimentos/efectos de los fármacos , Glucuronatos/metabolismo , Glucuronosiltransferasa/metabolismo , Glutatión/metabolismo , Crecimiento/efectos de los fármacos , Radioisótopos de Yodo , Masculino , Microsomas Hepáticos/efectos de los fármacos , Microsomas Hepáticos/enzimología , Dibenzodioxinas Policloradas/farmacología , Ratas , Ratas EndogámicasRESUMEN
Young male Wistar rats received single i.p. injections of 3,3',4,4',5,5'-hexabromobiphenyl. In rats dosed with 40 mg/kg, food consumption and growth as well as liver retinol and retinyl palmitate concentrations decreased, while serum retinol and liver weight increased within 28 days following the injection. In rats receiving a 20-mg/kg dose, food consumption, growth, liver weight, and serum retinol were not affect, although liver retinol and retinyl palmitate concentrations declined to 23 and 21% of their respective control values. Vitamin A metabolism was studied in liver microsomes prepared from rats sacrificed 7 days after the 20-mg/kg injection. The rate of retinoic acid hydroxylation via the cytochrome P-450 system to 4-hydroxyretinoic acid plus the subsequent oxidation to 4-ketoretinoic acid was significantly elevated. Retinoic acid conjugation by UDP-glucuronyl transferase was also significantly increased. These changes corresponded with increased activities of cytochrome P-450-dependent aryl hydrocarbon hydroxylase and UDP-glucuronyltransferase conjugation of p-nitrophenol. These results provide a direct link between enzyme induction due to xenobiotics and specific steps in the vitamin A metabolic pathway.
Asunto(s)
Hígado/efectos de los fármacos , Bifenilos Polibrominados/farmacología , Tretinoina/metabolismo , Animales , Sistema Enzimático del Citocromo P-450/metabolismo , Diterpenos , Glucuronosiltransferasa/metabolismo , Hidroxilación , Hígado/metabolismo , Masculino , Nitrofenoles/metabolismo , Ratas , Ratas Endogámicas , Ésteres de Retinilo , Tretinoina/análogos & derivados , Tretinoina/sangre , Vitamina A/análogos & derivados , Vitamina A/metabolismoRESUMEN
Poultry chicks receiving a low vitamin A semipurified diet and exposed to 3,4,3',4'-tetrachlorobiphenyl became hypothyroid in comparison with unexposed controls. Metabolic rate, total serum thyroxine, total serum triiodothyronine, and food intake decreased significantly while thyroid weight increased. Unexpectedly, growth rate was not affected on this diet. In the case of chicks receiving a low vitamin A--low iodine semipurified diet and exposed to the PCB congener, the hypothyroid response was apparently antagonized. Comparing exposed chicks with unexposed controls, metabolic rate and the proportion of free T3 (i.e., %T3 resin uptake) increased while total serum thyroxine and thyroid weight were unchanged. In addition, growth rate, food consumption, and serum retinol decreased on this diet. These results are interpreted to mean that growth rate may have been altered by circulating levels of retinol, and vitamin A insufficiency may predispose birds to the hypothyroid effects of PCBs.