SCS 6th Annual Meeting-EEVFA-11th International Congress of Biochemistry and Physiology of Exercise, Athens, Greece, 2023.

On behalf of the Strength and Conditioning Society (SCS) and the Hellenic Society of Biochemistry and Exercise Physiology (EEVFA), we are pleased to present the abstracts of the SCS 6th Annual Meeting and EEVFA-11th International Congress of Biochemistry and Physiology of Exercise. The event was held at the Hellenic Olympic Committee headquarters in Athens, Greece, on 19-22 October 2023, and comprised several invited sessions from international and national speakers on a variety of topics related to biochemistry and exercise physiology, strength and conditioning practices and their application to health, injury prevention and sports performance. These included strength training in high-performance sports, sport science and training-competition load management in elite environments, biochemistry and exercise physiology and prescription, nutrition and biomechanics, among others. The conference also included different practical workshops conducted by renowned academics and practitioners on eccentric training, change of direction ability and strength and power training in professional team-sports, and ergospirometry and exercise prescription in specific populations. Finally, the event disseminated up-to-date strength and conditioning research by providing practitioners and researchers with the opportunity to present their most recent findings. In this regard, all abstracts of the communications presented at the SCS 6th Annual Meeting-EEVFA-11th International Congress of Biochemistry and Physiology of Exercise can be found in this Conference Report.

Does Total Playing Time Affect the Neuromuscular, Physiological, and Subjective Recovery of Futsal Players during a Congested Period?

The aims of this study were to analyze the effects of a congested period (three games in four days) on countermovement (CMJ) jump-landing metrics, heart rate variability (HRV), and total recovery quality (TQR) score in under-19 male futsal players, and to detect the differences between those who played for more minutes (HIGHMIN) and less minutes (LOWMIN). Fourteen youth futsal players (age: 17.5 ± 0.5 years; body mass: 70.2 ± 8.5 kg; height: 1.80 ± 0.1 m) participated. HRV, TQR questionnaire, and CMJ metrics (i.e., CMJ height, relative peak power (PPREL), eccentric and concentric impulse, braking time, and time to peak force) were registered. A linear mixed model and effect sizes (ESs) were used to assess the differences between groups and days. Considering the total sample, a significant decrease was found in the PPREL and TQR score (p = 0.001-0.013 and ES = 0.28-0.99) on Days 2, 3, and 4 when compared to Day 1. HIGHMIN group presented a significant decrease in PPREL on Day 3 (p = 0.004; ES: 0.62; 95% CI: 0.39-2.65) when compared to Day 1, and in the TRQ score on Day 3 (p = 0.002; ES: 1.98; 95% CI: 0.18-2.46) and 4 (p = 0.003; ES: 2.25; 95% CI: 0.52-3.38) when compared to Day 1. Non-significant differences were found for the rest of the metrics and in the group LOWMIN. In summary, neuromuscular performance (i.e., CMJ PPREL) and subjective recovery were impaired in players with higher playing minutes during a match-congested period when compared to those with less on-court time.

Graphene oxide and oxidized carbon nanodiscs as biomedical scaffolds for the targeted delivery of quercetin to cancer cells.

Targeting cancer cells without affecting normal cells poses a particular challenge. Nevertheless, the utilization of innovative nanomaterials in targeted cancer therapy has witnessed significant growth in recent years. In this study, we examined two layered carbon nanomaterials, graphene and carbon nanodiscs (CNDs), both of which possess extraordinary physicochemical and structural properties alongside their nano-scale dimensions, and explored their potential as nanocarriers for quercetin, a bioactive flavonoid known for its potent anticancer properties. Within both graphitic allotropes, oxidation results in heightened hydrophilicity and the incorporation of oxygen functionalities. These factors are of great significance for drug delivery purposes. The successful oxidation and interaction of quercetin with both graphene (GO) and CNDs (oxCNDs) have been confirmed through a range of characterization techniques, including FTIR, Raman, and XPS spectroscopy, as well as XRD and AFM. In vitro anticancer tests were conducted on both normal (NIH/3T3) and glioblastoma (U87) cells. The results revealed that the bonding of quercetin with GO and oxCNDs enhances its cytotoxic effect on cancer cells. GO-Quercetin and oxCNDs-Quercetin induced G0/G1 cell cycle arrest in U87 cells, whereas oxCNDs caused G2/M arrest, indicating a distinct mode of action. In long-term survival studies, cancer cells exhibited significantly lower viability than normal cells at all corresponding doses of GO-Quercetin and oxCNDs-Quercetin. This work leads us to conclude that the conjugation of quercetin to GO and oxCNDs shows promising potential for targeted anticancer activity. However, further research at the molecular level is necessary to substantiate our preliminary findings.

Carbon Nanodisks Decorated with Guanidinylated Hyperbranched Polyethyleneimine Derivatives as Efficient Antibacterial Agents.

Non-toxic carbon-based hybrid nanomaterials based on carbon nanodisks were synthesized and assessed as novel antibacterial agents. Specifically, acid-treated carbon nanodisks (oxCNDs), as a safe alternative material to graphene oxide, interacted through covalent and non-covalent bonding with guanidinylated hyperbranched polyethyleneimine derivatives (GPEI5K and GPEI25K), affording the oxCNDs@GPEI5K and oxCNDs@GPEI25K hybrids. Their physico-chemical characterization confirmed the successful and homogenous attachment of GPEIs on the surface of oxCNDs, which, due to the presence of guanidinium groups, offered them improved aqueous stability. Moreover, the antibacterial activity of oxCNDs@GPEIs was evaluated against Gram-negative E. coli and Gram-positive S. aureus bacteria. It was found that both hybrids exhibited enhanced antibacterial activity, with oxCNDs@GPEI5K being more active than oxCNDs@GPEI25K. Their MIC and MBC values were found to be much lower than those of oxCNDs, revealing that the GPEI attachment endowed the hybrids with enhanced antibacterial properties. These improved properties were attributed to the polycationic character of the oxCNDs@GPEIs, which enables effective interaction with the bacterial cytoplasmic membrane and cell walls, leading to cell envelope damage, and eventually cell lysis. Finally, oxCNDs@GPEIs showed minimal cytotoxicity on mammalian cells, indicating that these hybrid nanomaterials have great potential to be used as safe and efficient antibacterial agents.

Exploring countermovement jump variables across competitive levels and playing positions in futsal.

Introduction: The aims of this study were to compare several countermovement jump (CMJ) kinetic variables between professional (PRO) and semi-professional (SEMI-PRO) futsal players and examine the differences amongst playing positions. Methods: CMJ performance from 56 male futsal players (25.2 ± 4.8 years; weight: 74.4 ± 6.4 kg) was analysed. Players were separated into PRO (n = 29; 27.0 ± 4.4 years; 75.4 ± 6.0 kg) and SEMI-PRO (n = 27; 22.7 ± 4.3 years; 73.1 ± 6.8 kg), and according to playing position: defenders (n = 16; 25.4 ± 3.7 years; 75.2 ± 6.0 kg), wingers (n = 26; 23.5 ± 4.5 years; 72.0 ± 6.9 kg), and pivots (n = 14; 28.0 ± 5.6 years; 77.8 ± 4.3 kg). Linear mixed models and effect sizes were used for the analyses based on the mean of two jumps for each variable. Results: PRO players presented a deeper center of mass (COM) displacement (p = 0.002, ES = 0.83), greater eccentric (Ecc) absolute (p = 0.019, ES = 0.61) and relative peak power (p = 0.046, ES = 0.52), and achieved greater Ecc peak velocities (p = 0.004, ES = 0.76) when compared to SEMI-PRO. Non-significant and trivial-to-small differences were observed in all the other CMJ variables according to the competitive level and playing position. Discussion: Ecc capabilities (i.e., deeper COM displacement, greater Ecc absolute and relative peak power, and peak velocity) during vertical jump seem to differentiate PRO and SEMI-PRO players. However, CMJ variables do not discriminate amongst playing positions in futsal players.

The effect of menthol rinsing before intermittent exercise on physiological, physical, and thermo-behavioral responses of male football referees in hot and humid environment.

Introduction: In the current experiment, we aimed to evaluate whether eliciting pre-exercise non-thermal cooling sensations would alter perceptual measures, and physical and physiological responses in football referees. Methods: Nine highly trained male football referees undertook two 45-minute intermittent exercise protocols in hot and humid conditions (34.2 ± 0.6°C, 62.5 ± 1.0% relative humidity). In a randomized counterbalanced crossover design, 1 of 2 beverages were given before the warm-up: a 0.01% menthol solution or a placebo noncaloric solution. Physical performance was quantified as total distance covered in each of the three 15-minute exercise blocks. Core temperature, heart rate, thermal sensation and thermal comfort were measured at rest and after each exercise block. Results: No changes were observed between trials and over time for distance covered. No main effect of mouth rinse was observed for core temperature and heart rate, but both increased over time in all conditions (P < 0.001). Thermal sensation and thermal comfort were significantly improved with menthol after mouth-rinsing (P < 0.05), but with no differences at any other time-point. Discussion: These results indicate that non-thermal cooling oral stimuli provide immediate behavioral changes but may not influence physiological or physical responses in football referees, during intermittent exercise in hot and humid environments. Clinical Trial Registration: www.clinicaltrials.gov, identifier NCT05632692.

Eco-friendly spark-generated CoxOy nanoparticle-modified graphite screen-printed sensing surfaces for the determination of H2O2 in energy drinks.

The modification of graphite screen-printed electrodes (SPEs) is reported using an eco-friendly and extremely fast method based on the direct cobalt pin electrode-to-SPE spark discharge at ambient conditions. This approach does not utilize any liquids or chemical templates, does not produce any waste, and allows the in-situ generation of CoxOy nanoparticles onto the electrode surface and the development of efficient electrocatalytic sensing surfaces for the determination of H2O2. Co-spark SPEs were characterized using scanning electron microscopy, energy-dispersive X-ray spectroscopy and x-ray photoelectron spectroscopy (XPS), revealing the formation of surface confined CoxOy nanoparticles and the diverse oxidation states of cobalt species. Co-spark SPEs were also characterized with cyclic voltammetry and electrochemical impedance spectroscopy. Redox transitions of the surface confined electrocatalysts are demonstrated by electrochemical polarization studies, showing the formation of different oxides (CoxOy), varying the XPS results. Amperometric measurements at 0.3 V vs. Ag/AgCl revealed a linear relationship between the current response and the concentration of H2O2 over the range 1 - 102 µM, achieving a limit of detection (3σ/m) of 0.6 µM. The interference effect of various electroactive species was effectively addressed by employing dual measurements in the absence and presence of the enzyme catalase. The analytical utility of the method was evaluated in antioxidant rich real-world samples, such as energy drinks, demonstrating sufficient recovery.

Effect of Highly Hydrophilic Superparamagnetic Iron Oxide Nanoparticles on Macrophage Function and Survival.

Superparamagnetic iron oxide nanoparticles (SPIONs) have garnered significant attention in the medical sector due to their exceptional superparamagnetic properties and reliable tracking capabilities. In this study, we investigated the immunotoxicity of SPIONs with a modified surface to enhance hydrophilicity and prevent aggregate formation. The synthesized SPIONs exhibited a remarkably small size (~4 nm) and underwent surface modification using a novel "haircut" reaction strategy. Experiments were conducted in vitro using a human monocytic cell line (THP-1). SPIONs induced dose-dependent toxicity to THP-1 cells, potentially by generating ROS and initiating the apoptotic pathway in the cells. Concentrations up to 10 µg/mL did not affect the expression of Nrf2, HO-1, NF-κB, or TLR-4 proteins. The results of the present study demonstrated that highly hydrophilic SPIONs were highly toxic to immune cells; however, they did not activate pathways of inflammation and immune response. Further investigation into the mechanisms of cytotoxicity is warranted to develop a synthetic approach for producing effective, highly hydrophilic SPIONs with little to no side effects.

Interpreting match performance in elite futsal: considerations for normalizing variables using effective time.

Introduction: This study aimed to investigate the physical and individual technical-tactical performance of elite futsal players. Methods: Thirteen National Team futsal players (27.4 ± 4.7 years, 70.3 ± 7.6 kg, 176.3 ± 5.5 cm) competed in 15 official matches in three distinct competitions. Session rate of perceived exertion (sRPE) and player load (PL) were measured for assessing physical internal and external load, respectively. Individual tactical actions were analyzed using Instat Scout®, considering variables such as fouls, shots, shots on target, goals, successful dribbles, passes, successful passes, ball losses, ball recoveries, and challenges won. Results: The cluster analysis classified the players into two groups: "high-involvement" (HIGH) and "low-involvement" (LOW), based on their average effective playing time, sRPE, and PL. A linear mixed model was used to examine the differences in external and internal load and technical-tactical variables between the two groups, accounting for individual repeated measures. Considering absolute values, HIGH players presented higher sRPE, RPE, total PL, shots, goals, passes, successful passes, ball losses and recoveries, and challenges won (p = 0.001-0.039; ES = 0.43-2.48), when compared to their LOW counterparts. However, when analyzed relative to effective time, LOW players reached higher total PL and shots per minute (p = 0.001-0.012; ES = 0.39-0.93) when compared to HIGH players. Non-significant differences were found for the rest of the variables. Discussion: In summary, these results emphasize the importance of normalizing internal and external load variables and technical-tactical performance variables per effective playing time in futsal. The current findings indicate that players with lower involvement can present the same or even higher physical and technical-tactical performance compared to teammates with more playing time.

Menthol mouth rinsing and performance in elite football referees in the heat: A study protocol for a randomized crossover trial.

Background: Within professional European competitions, matches can be played in extreme environmental temperatures, ranging from -5 °C to +30 °C in different countries. Furthermore, the World Cups are usually played in the summer months, when temperatures can exceed 35 °C, increasing physiological stress. Practical and cost-effective cooling strategies may be implemented to help players and referees to cope with exercising in the heat. No study has evaluated the effect of non-thermal internal cooling techniques regarding performance responses on elite football referees, so far. This study aims to analyse the effects of a menthol mouth rinse regarding physical, physiological, and perceptual performance in elite male football referees, during a 90-min football protocol in the heat. Methods: At least thirteen male football referees will be recruited to perform two intermittent football protocols, separated by no less than 7 days. After passing the eligibility criteria, the participants will be randomly assigned to 1 of 2 beverages: (1) intervention - menthol solution 0.01% and (2) placebo - noncaloric berry-flavored solution, both at room temperature. The beverages will be given before warm-up (pre-cooling) and at the half-time (per-cooling). The trials will follow a randomized counterbalanced crossover design, single blinded, and will take place in indoor facilities, with Wet Bulb Globe Temperature (WBGT) > 30 °C, at the same time of the day to control for circadian variations. Impact of the project: The results of this study are expected to determine whether mouth rinsing a menthol solution before and during a football exercise protocol performed in the heat will alter perceptual measures and help ease physiological strain and attenuate performance decrements in elite male football referees, comparing to a non-cooling strategy. Thus, we can be closer to defining nutritional strategies of internal cooling that may be an advantage for the performance of the football referees in the heat. Trial registration: www.ClinicalTrials.gov NCT05632692 registered on 20 November 2022.

Effective playing time affects technical-tactical and physical parameters in football.

Effective playing time has been debated as a topic of major concern in football. Thus, the current experimental study aimed to investigate the effects of effective playing time on technical-tactical and physical match parameters in football. One hundred and seventy-nine male highly trained football players (aged 27.9 ± 5.1 years) from twelve teams performed two different match-play conditions: 45 min of match-play without stopping the chronometer (T45), and 30 min of match-play by stopping the chronometer every time the ball was out-of-play (T30). T30 presented a significantly higher total time (49:30 vs. 45:00 min; p = <.001; ES = 0.76), effective playing time (28:70 vs. 26:80 min; p = <.001; ES = 0.62), and stopped time (20:60 vs. 18:20 min; p = 0.003; ES = 0.38) compared to T45. Total ball possession (54.4% vs. 45.6%; p = 0.002) and 1/3 ball possession (55.3% vs. 44.7%; p = 0.018) was higher in T30 condition when compared to T45. Regarding match external load, total distance covered (4,899 vs. 4,481 m; p = <.001; ES = 0.71), moderate-speed running (607 vs. 557 m; p = 0.002; ES = 0.26) and high-speed running (202 vs. 170 m; p = <.001; ES = 0.33), high-speed activities (284 vs. 245 m; p = 0.003; ES = 0.24), accelerations (27 vs. 24; p = <.001; ES = 0.32), and decelerations (31 vs. 28; p = 0.005; ES = 0.26) were higher in T30 compared to T45. In conclusion, these findings suggest that higher effective playing time may affect technical-tactical and physical parameters during football games.

Reliability of Maximal Strength and Peak Rate of Force Development in a Portable Nordic Hamstrings Exercise Device.

The Nordic hamstring exercise (NHE) is a very popular exercise used to improve eccentric strength and prevent injuries. The aim of this investigation was to assess the reliability of a portable dynamometer that measures maximal strength (MS) and rate of force development (RFD) during the NHE. Seventeen physically active participants (34.8 ± 4.1 years; n = 2 women and n = 15 men) participated. Measurements occurred on two different days separated by 48-72 h. Test-retest reliability was calculated for bilateral MS and RFD. No significant test-retest differences were observed in NHE (test-retest [95% CI, confidence interval]) for MS [-19.2 N (-67.8; 29.4); p = 0.42] and RFD [-70.4 N·s-1 (-178.4; 37.8); p = 0.19]. MS showed high reliability (intraclass correlation coefficient [ICC] [95% CI], =0.93 [0.80-0.97] and large within-subject correlation between test and retest [r = 0.88 (0.68; 0.95)]. RFD displayed good reliability [ICC = 0.76 (0.35; 0.91)] and moderate within-subject correlation between test and retest [r = 0.63 (0.22; 0.85)]. Bilateral MS and RFD displayed a coefficient of variation of 3.4% and 4.6%, respectively, between tests. The standard error of measurement and the minimal detectable change for MS was 44.6 arbitrary units (a.u.) and 123.6 a.u., and 104.6 a.u. and 290.0 a.u. for peak RFD. This study shows that MS and RFD can be measured for NHE using a portable dynamometer. However, not all exercises are suitable to apply to determine RFD, so caution must be taken when analyzing RFD during NHE.

Effects of high-intensity interval training versus sprint interval training during the second wave of COVID-19 lockdown on soccer players

This study aimed to compare the effects of two intervention programs (i.e., high-intensity interval training [ΗΙΙΤ] versus sprint interval training [SIT]) during the second wave of pandemic COVID-19 in semi-professional soccer players. Twenty-nine male soccer players were divided into two groups: HIIT (N: 16, age: 19.6 ± 2.4 years, height: 1.8 ± 0.5 m, weight: 71.5 ± 4.6 kg, and body fat: 7.3 ± 2.6%), who performed a HIIT protocol with progressive intensity and resistance training; SIT (N: 13, age: 23.5 ± 5.1 years, height: 1.8 ± 0.1 m, weight: 74.0 ± 4.5 kg and body fat: 8.8 ± 2.6%) that performed a SIT program combined with body weight circuit training. According to the instructions from World Health Organization of basic protective measures against COVID-19, each session did not exceed 60 min. Aerobic capacity (Yo–Yo IR1) and countermovement jump (CMJ) height were measured before and after a 4-week intervention period. Aerobic variables (maximum oxygen uptake [V̇O2max], velocity at V̇O2max [vV̇O2max] and CMJ significantly increased (p<0.001), while maximum heart rate [HRmax] decreased (p<0.01) following the HIIT intervention program. Conversely, values remained unchanged in the SIT group after the training. Post-evaluation between-group comparisons revealed that V̇O2max was significantly higher in HIIT when compared to SIT (p<0.05). These results suggest that HIIT combined with resistance training, in different sessions, could lead to superior improvements in endurance and vertical jump ability in short time periods when compared to the combination of SIT and body-weighted strength training in semi-professional soccer players. (AU)

Tyrosinase Magnetic Cross-Linked Enzyme Aggregates: Biocatalytic Study in Deep Eutectic Solvent Aqueous Solutions.

In the field of biocatalysis, the implementation of sustainable processes such as enzyme immobilization or employment of environmentally friendly solvents, like Deep Eutectic Solvents (DESs) are of paramount importance. In this work, tyrosinase was extracted from fresh mushrooms and used in a carrier-free immobilization towards the preparation of both non-magnetic and magnetic cross-linked enzyme aggregates (CLEAs). The prepared biocatalyst was characterized and the biocatalytic and structural traits of free tyrosinase and tyrosinase magnetic CLEAs (mCLEAs) were evaluated in numerous DES aqueous solutions. The results showed that the nature and the concentration of the DESs used as co-solvents significantly affected the catalytic activity and stability of tyrosinase, while the immobilization enhanced the activity of the enzyme in comparison with the non-immobilized enzyme up to 3.6-fold. The biocatalyst retained the 100% of its initial activity after storage at -20 °C for 1 year and the 90% of its activity after 5 repeated cycles. Tyrosinase mCLEAs were further applied in the homogeneous modification of chitosan with caffeic acid in the presence of DES. The biocatalyst demonstrated great ability in the functionalization of chitosan with caffeic acid in the presence of 10% v/v DES [Bet:Gly (1:3)], enhancing the antioxidant activity of the films.

Does Green Exfoliation of Graphene Produce More Biocompatible Structures?

Graphene has been studied thoroughly for its use in biomedical applications over the last decades. A crucial factor for a material to be used in such applications is its biocompatibility. Various factors affect the biocompatibility and toxicity of graphene structures, including lateral size, number of layers, surface functionalization, and way of production. In this work, we tested that the green production of few-layer bio-graphene (bG) enhances its biocompatibility compared to chemical-graphene (cG). When tested against three different cell lines in terms of MTT assays, both materials proved to be well-tolerated at a wide range of doses. However, high doses of cG induce long-term toxicity and have a tendency for apoptosis. Neither bG nor cG induced ROS generation or cell cycle modifications. Finally, both materials affect the expression of inflammatory proteins such as Nrf2, NF-kB and HO-1 but further research is required for a safe result. In conclusion, although there is little to choose between bG and cG, bG's sustainable way of production makes it a much more attractive and promising candidate for biomedical applications.

Effects of Concurrent High-Intensity and Strength Training on Muscle Power and Aerobic Performance in Young Soccer Players during the Pre-Season.

The aim of the present study was to evaluate two different intervention programs applied during a 4-week pre-season period. Twenty-nine players participated in this study and were divided into two groups. One group (BallTrain, n = 12, age: 17.8 ± 0.4 years, body mass: 73.9 ± 7.6 kg, height: 178 ± 0.1 cm, body fat: 9.6 ± 5.3%) performed a higher percentage of aerobic training with ball and strength training using plyometrics and exercises with body weight. The other group (HIITTrain, n = 17, age: 17.8 ± 0.7 years, body mass: 73.3 ± 5.0 kg, height: 179 ± 0.1 cm, body fat: 8.0 ± 2.3%) trained with high-intensity interval training (HIIT) without the ball and performed resistance training with weights in the same session. Both groups trained for strength (two times/week) and performed aerobic-anaerobic fitness without the ball, passing games, and tactical and small-sided games. Lower limb power (CMJ) and aerobic fitness (Yo-Yo intermittent recovery test level 1-IR1) were evaluated before and after the four-week training program. Yo-Yo IR1 performance was improved in both groups, but the improvement was greater for the HIITTrain than BallTrain group (468 ± 180 vs. 183 ± 177 m, p = 0.07). CMJ showed a non-significant improvement in the BallTrain group (5.8 ± 8.8%, p = 0.16), but it decreased by 8.1 ± 9% (p = 0.001), in the HIITTrain group. In conclusion, we have shown that a short pre-season period of training results in improvements in aerobic fitness in both groups, with high-intensity interval training showing superior adaptations than training with the ball. However, CMJ performance was reduced in this group, possibly suggesting higher fatigue levels and overload, and/or showing the effects of concurrent HIITTrain and strength training in soccer.

Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells.

Carbon nanotubes (CNTs) possess excellent physicochemical and structural properties alongside their nano dimensions, constituting a medical platform for the delivery of different therapeutic molecules and drug systems. Hydroxytyrosol (HT) is a molecule with potent antioxidant properties that, however, is rapidly metabolized in the organism. HT immobilized on functionalized CNTs could improve its oral absorption and protect it against rapid degradation and elimination. This study investigated the effects of cellular oxidized multiwall carbon nanotubes (oxMWCNTs) as biocompatible carriers of HT. The oxidation of MWCNTs via H2SO4 and HNO3 has a double effect since it leads to increased hydrophilicity, while the introduced oxygen functionalities can contribute to the delivery of the drug. The in vitro effects of HT, oxMWCNTS, and oxMWCNTS functionalized with HT (oxMWCNTS_HT) were studied against two different cell lines (NIH/3T3 and Tg/Tg). We evaluated the toxicity (MTT and clonogenic assay), cell cycle arrest, and reactive oxygen species (ROS) formation. Both cell lines coped with oxMWCNTs even at high doses. oxMWCNTS_HT acted as pro-oxidants in Tg/Tg cells and as antioxidants in NIH/3T3 cells. These findings suggest that oxMWCNTs could evolve into a promising nanocarrier suitable for targeted drug delivery in the future.

Neuromuscular Performance Changes in Elite Futsal Players Over a Competitive Season.

ABSTRACT: Spyrou, K, Alcaraz, PE, Marín-Cascales, E, Herrero-Carrasco, R, Cohen, DD, and Freitas, TT. Neuromuscular performance changes in elite futsal players over a competitive season. J Strength Cond Res 37(5): 1111-1116, 2023-A professional futsal season imposes a great amount of physiological and mechanical stress on players. The main aim of this study was to examine the changes in neuromuscular performance qualities across the season. Ten professional male players performed a 10-m sprint, standing long jumps (SLJs), and countermovement jumps (CMJs) during the competitive season (i.e., every ∼5 weeks from September to January). A one-way repeated measures ANOVA with post hoc pairwise comparisons and effect sizes (ESs) were used to analyze potential differences among these assessments. A significant and large decline was found in concentric peak power ( p = 0.040; ES = 1.24). A nonsignificant and moderate decrease was observed in sprint ability ( p = 0.155; ES = 1.03), CMJ height ( p = 0.175; ES = 1.00), and SLJ distance ( p = 0.164; ES = 1.03). Regarding other CMJ kinetic variables, nonsignificant and moderate changes were found. In summary, considering the neuromuscular performance tests and variables assessed, only concentric peak power in CMJ decreased significantly across the season; however, nonsignificant decrements were observed in sprinting time, SLJ, CMJ height, and other kinetic metrics. CMJ variables during the jump-land cycle should be incorporated alongside more traditional measures (e.g., jump height) to monitor performance during the season.

Low Overpotential Electrochemical Reduction of CO2 to Ethanol Enabled by Cu/CuxO Nanoparticles Embedded in Nitrogen-Doped Carbon Cuboids.

The electrochemical conversion of CO2 into value-added chemicals is a promising approach for addressing environmental and energy supply problems. In this study, electrochemical CO2 catalysis to ethanol is achieved using incorporated Cu/CuxO nanoparticles into nitrogenous porous carbon cuboids. Pyrolysis of the coordinated Cu cations with nitrogen heterocycles allowed Cu nanoparticles to detach from the coordination complex but remain dispersed throughout the porous carbon cuboids. The heterogeneous composite Cu/CuxO-PCC-0h electrocatalyst reduced CO2 to ethanol at low overpotential in 0.5 M KHCO3, exhibiting maximum ethanol faradaic efficiency of 50% at -0.5 V vs. reversible hydrogen electrode. Such electrochemical performance can be ascribed to the synergy between pyridinic nitrogen species, Cu/CuxO nanoparticles, and porous carbon morphology, together providing efficient CO2 diffusion, activation, and intermediates stabilization. This was supported by the notably high electrochemically active surface area, rich porosity, and efficient charge transfer properties.

Is graphene the rock upon which new era continuous glucose monitors could be built?

Diabetes mellitus' (DM) prevalence worldwide is estimated to be around 10% and is expected to rise over the next decades. Monitoring blood glucose levels aims to determine whether glucose targets are met to minimize the risk for the development of symptoms related to high or low blood sugar and avoid long-term diabetes complications. Continuous glucose monitoring (CGMs) systems emerged almost two decades ago and have revolutionized the way diabetes is managed. Especially in Type 1 DM, the combination of a CGM with an insulin pump (known as a closed-loop system or artificial pancreas) allows an autonomous regulation of patients' insulin with minimal intervention from the user. However, there is still an unmet need for high accuracy, precision and repeatability of CGMs. Graphene was isolated in 2004 and found immediately fertile ground in various biomedical applications and devices due to its unique combination of properties including its high electrical conductivity. In the last decade, various graphene family nanomaterials have been exploited for the development of enzymatic and non-enzymatic biosensors to determine glucose in biological fluids, such as blood, sweat, and so on. Although great progress has been achieved in the field, several issues need to be addressed for graphene sensors to become a predominant material in the new era of CGMs.