Potassium transporter OsHAK17 may contribute to saline-alkaline tolerant mechanisms in rice (Oryza sativa).

Rice production is seriously affected by saline-alkaline stress worldwide. To elucidate the saline-alkaline tolerance mechanisms in a novel tolerant rice variety, Shwe Nang Gyi (SNG), we investigated ion accumulation in SNG and Koshihikari (KSH), which is a saline-alkaline sensitive rice variety, and the candidates for saline-alkaline inducible genes in SNG using RNA-seq. SNG had superior ion accumulation capacity, such as K and Zn, compared to KSH. In contrast, SNG accumulated the same level of Na content in its leaf blades as KSH despite the higher dry weight of the SNG leaf blades. We further found that the expression of numerous genes, including several K+ transporter/high-affinity K+ transporter/K+ uptake protein/K+ transporter (HAK/KUP/KT) family members, were upregulated in SNG, and that OsHAK17 and OsHAK21 expression levels in the roots were significantly higher in SNG than in KSH. Moreover, yeast complementation analysis revealed that OsHAK17 was involved in K+ uptake under high-Na conditions. These results suggested that SNG has an effective K+ acquisition system supported by OsHAK17 functioning in saline-alkaline environments.

Potential usage of biosynthesized zinc oxide nanoparticles from mangosteen peel ethanol extract to inhibit Xanthomonas oryzae and promote rice growth.

In recent decades, the biosynthesis of nanoparticles using biological agents, such as plant extracts, has grown in popularity due to their environmental and economic benefits. Therefore, this study investigated into utilizing ethanol crude extract sourced from mangosteen peel for the synthesis of zinc oxide nanoparticles (ZnO NPs) and assessing their efficacy against the rice blight pathogen (Xanthomonas oryzae pv. oryzae) through antibacterial evaluations. Additionally, the effects of the synthesized ZnO NPs on rice plant growth was investigated. The X-ray diffraction analysis revealed the production of wurtzite ZnO NPs under specific synthesis conditions, exhibiting a crystallite size of 38.71 nm (or 387.122 Å) without any contamination. Analysis of the ultraviolet-visible optical absorption spectrum indicated a characteristic absorption peak at 363 nm, suggesting a calculated band gap energy of 2.88 eV for the ZnO NPs. Furthermore, Fourier transform infrared spectroscopy analysis confirmed the presence of active compounds functional groups from mangosteen peel in the synthesized ZnO NPs. These biosynthesized ZnO NPs demonstrated significant inhibition of X. oryzae pv. oryzae growth, exhibiting an in vitro 50 % inhibitory concentration (IC50) value of 1.895 mg/mL and a minimum inhibitory concentration (MIC) value of 4 mg/mL. The ZnO NPs treatments at two-fold IC50 values significantly enhanced root length, dry biomass, and chlorophyll a content in rice plants. Consequently, the results demonstrated the potential application of biosynthesized ZnO NPs from mangosteen peel extract in green agriculture, as an alternative to excessive antibiotic use, for combating bacterial plant diseases, and for enhancing plant growth.

Comparative proteomic analysis of chromosome segment substitution lines of Thai jasmine rice KDML105 under short-term salinity stress.

MAIN CONCLUSIONS: Heat shock proteins, ROS detoxifying enzymes, and ion homeostasis proteins, together with proteins in carbohydrate metabolism, cell structure, brassinosteroids, and carotenoid biosynthesis pathway were up-regulated in CSSLs under salinity stress. Rice is one of the most consumed staple foods worldwide. Salinity stress is a serious global problem affecting rice productivity. Many attempts have been made to select or produce salinity-tolerant rice varieties. Genetics and biochemical approaches were used to study the salinity-responsive pathway in rice to develop salinity tolerant strains. This study investigated the proteomic profiles of chromosome segment substitution lines (CSSLs) developed from KDML105 (Khao Dawk Mali 105, a Thai jasmine rice cultivar) under salinity stress. The CSSLs showed a clear resistant phenotype in response to 150 mM NaCl treatment compared to the salinity-sensitive line, IR29. Liquid chromatography-tandem mass spectrometry using the Ultimate 3000 Nano/Capillary LC System coupled to a Hybrid Quadrupole Q-Tof Impact II™ equipped with a nano-captive spray ion source was applied for proteomic analysis. Based on our criteria, 178 proteins were identified as differentially expressed proteins under salinity stress. Protein functions in DNA replication and transcription, and stress and defense accounted for the highest proportions in response to salinity stress, followed by protein transport and trafficking, carbohydrate metabolic process, signal transduction, and cell structure. The protein interaction network among the 75 up-regulated proteins showed connections between proteins involved in cell wall synthesis, transcription, translation, and in defense responses.

Phenotypic Diversity of Root Characteristics in Recombinant Inbred Lines of Cross Between Lowland and Highland Rice Varieties for Drought Tolerance Potential.

<b>Background and Objective:</b> Breeding between highland and lowland rice varieties is one of the strategic breeding of lowland rice for enhancing drought-tolerant capacity through root structure improvement. The objective of this study was to evaluate the phenotypic diversity of rice root traits in pot screening compared to the lowland parent. <b>Materials and Methods:</b> The basket method was utilized in pot cultivation to evaluate the 100 of F7 Recombinant Inbred Lines (RILs) derived through single seed descent method from a cross between lowland rice, RD49 variety and upland rice, Payaleumgaeng (PLG) variety. The two parents and F7 progenies were evaluated for the number of shallow roots (SRN) and the number of deep roots (DRN), together with other traits which were the number of total roots (TRN), the Ratio of Deep Rooting (RDR), maximum Root Length (RL), Root Dry Weight (RDW), Shoot Dry Weight (SDW), the ratio of Root to Shoot Weight (RSR) and Plant Height (PH). <b>Results:</b> The result showed that PLG had significantly higher SRN, DRN, TRN and RDR than RD49. The distribution of these traits showed slightly positive skewness in DRN, RDR, RDW, SDW and RSR and negative skewness in SRN, TRN, RL and PH. However, some lines in this RIL population displayed better performance of root traits compared to both parents. Principal Component Analysis (PCA) of DRN, SRN, TRN and RDR in this population showed a distinctly different pattern among both parents. Most of the selected lines had superior RDR over RD49 and had various root characteristics patterns due to the diverse PCA coordinates. The yield trial of some breeding lines in this cross show superior yield over RD49 under drought-prone cultivation area. <b>Conclusion:</b> This study showed broad phenotypic diversity in the population constructed through single seed descent selection for enhancing deep root structure in rice for drought adaptation.

Different Rhizospheric pH Conditions Affect Nutrient Accumulations in Rice under Salinity Stress.

This study was conducted to determine the responses to saline-alkaline (SA) stress with regard to nutrient accumulation in two rice varieties having different tolerances to salt-stress. A salinity-tolerant landrace, Pokkali, and a salinity-sensitive variety, PTT1, were exposed to three levels of SA conditions, pH 7.0 (mild), pH 8.0 (moderate), and pH 9.0 (severe), under 50 mM Na stress. The results indicated that Pokkali had comparably greater SA tolerance than PTT1 owing to its higher biomass production. The maintenance of the lower Na/K ratio in Pokkali shoots was achieved by the higher expression of OsHKT1;5 encoding a Na+ transporter in the shoots, OsNHX1 encoding a tonoplast-localized Na+/H+ antiporter in the roots, and OsHAK16 encoding a K+ transporter in the roots under SA conditions. We propose that the high expression of Fe deficiency-responsive genes, OsIRT1, OsIRO2, OsYSL15, OsNAS1, and OsNAS2, in both rice varieties under all SA conditions should contribute to Fe homeostasis in the shoots. In addition, SA treatment increased the concentrations of Ca, Mn, Zn, and Cu in the roots but decreased their concentrations in the shoots of both varieties. Overall, the results indicated that high rhizospheric pH influenced nutrient uptake and translocation from the roots to the shoots in rice.