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The CCAAT enhancer-binding protein α (C/EBPα) plays an important role in myeloid cell differentiation and in the enhancement of C/EBPα expression/activity, which can lead to granulocytic differentiation in acute myeloid leukemia (AML) cells. We found that styryl quinazolinones induce upregulation of C/EBPα expression, and thereby induce myeloid differentiation in human myeloid leukemia cell lines. We screened a series of active styryl quinazolinones and evaluated the structureâ»activity relationship (SAR) of these small molecules in inducing C/EBPα expression-thereby prompting the leukemic cells to differentiate. We observed that compound 78 causes differentiation at 3 µM concentration, while 1 induces differentiation at 10 µM concentration. We also observed an increase in the expression of neutrophil differentiation marker CD11b upon treatment with 78. Both the C/EBPα and C/EBPε levels were found to be upregulated by treatment with 78. These SAR findings are inspiration to develop further modified styryl quinazolinones, in the path of this novel differentiation therapy, which can contribute to the care of patients with AML.
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Proteína alfa Potenciadora de Unión a CCAAT/metabolismo , Quinazolinonas/farmacología , Estirenos/farmacología , Apoptosis/efectos de los fármacos , Antígeno CD11b/metabolismo , Diferenciación Celular/efectos de los fármacos , Granulocitos/efectos de los fármacos , Granulocitos/patología , Células HL-60 , Hematopoyesis , Humanos , Leucemia Mieloide Aguda/metabolismo , Leucemia Mieloide Aguda/patología , Bibliotecas de Moléculas Pequeñas/química , Relación Estructura-Actividad , Activación Transcripcional , Regulación hacia ArribaRESUMEN
Medical devices form a broad range of appliances from a basic nanoparticle coating or surgical gloves to a complicated laser therapy device. These devices are designed to support patients, surgeons and healthcare personnel in meeting patients' healthcare needs. Regulatory authorities of each country regulate the process of approval, manufacturing and sales of these medical devices so as to ensure safety and quality to patients or users. Recent recalls of medical devices has increased importance of safety, awareness and regulation of the devices. Singapore and India have strong presence and national priorities in medical devices development and use. Herein we capture the rationale of each of these national regulatory bodies and compare them with the medical devices regulatory practices of USA and European nations. Apart from the comparison of various regulatory aspects, this review will specifically throw light on the polymer material based medical devices and their safety.
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Aprobación de Recursos , Legislación de Dispositivos Médicos , Polímeros/química , Animales , Diseño de Equipo , Equipos y Suministros , Regulación Gubernamental , HumanosRESUMEN
Pharmaceutically active compounds require different modes of drug delivery systems to accomplish therapeutic activity without loss of its activity and lead to exhibit no adverse effects. Originating from ancient days, pulmonary mode of drug delivery is gaining much importance compared to other modes of drug delivery systems with respect to specific diseases. Pulmonary drug delivery is a non-invasive route for local and systemic therapies together with more patient convenience, compliance and is a needleless system. In this review, we addressed the vaccine delivery via non- or minimally invasive routes. Polymeric nanoparticles are preferred for use in the pulmonary delivery devices owing to a prolonged retention in lungs. Small site for absorption, mucociliary clearance, short residence time and low bioavailability are some of the limitations in pulmonary drug delivery have been resolved by generating micro- and nano-sized aerosol particles. We have classified the breathable medicine on the basis of available devices for inhalation and also prominent diseases treated through pulmonary mode of drug delivery. Owing to increasing toxicity of pharmacological drugs, the use of natural medicines has been rapidly gaining importance recently. The review article describes breathability of medicines or the pulmonary mode of drug delivery system and their drug release profile, absorption, distribution and efficacy to cure asthma and diabetes.
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Administración por Inhalación , Sistemas de Liberación de Medicamentos , Nanomedicina , Nanopartículas/administración & dosificación , Humanos , Pulmón/metabolismo , Pulmón/fisiología , Extractos Vegetales/administración & dosificación , Polvos , Vacunas/administración & dosificaciónRESUMEN
Cardiac tissue engineering promises to revolutionize the treatment of patients with end-stage heart failure and provide new solutions to the serious problems of shortage of heart donors. The influence of extracellular matrix (ECM) plays an influential role along with nanostructured components for guided stem cell differentiation. Hence, nanoparticle embedded Nanofibrous scaffolds of FDA approved polycaprolactone (PCL), Vitamin B12 (Vit B12), Aloe Vera(AV) and Silk fibroin(SF) was constructed to differentiate mesenchymal stem cells into cardiac lineage. Cardiomyocytes (CM) and Mesenchymal stem cells (MSC) were co-cultured on these fabricated nanofibrous scaffolds for the regeneration of infarcted myocardium. Results demonstrated that synthesized gold nanoparticles were of the size 16 nm and the nanoparticle loaded nanofibrous scaffold has a mechanical strength of 2.56 MPa matching that of the native myocardium. The gold nanoparticle blended PCL scaffolds were found to be enhancing the MSCs proliferation and differentiation into cardiogenesis. Most importantly the phenotype and cardiac marker expression in differentiated MSCs were highly resonated in gold nanoparticle loaded nanofibrous scaffolds. The appropriate mechanical strength provided by the functionalized nanofibrous scaffolds profoundly supported MSCs to produce contractile proteins and achieve typical cardiac phenotype.
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Diferenciación Celular , Oro/química , Células Madre Mesenquimatosas/citología , Nanopartículas del Metal , Miocitos Cardíacos/citología , Nanofibras , Animales , Técnicas de Cocultivo , Ensayo de Materiales , Microscopía Electrónica de Rastreo , ConejosRESUMEN
Temperature-triggered switchable nanofibrous membranes are successfully fabricated from a mixture of cellulose acetate (CA) and poly(N-isopropylacrylamide) (PNIPAM) by employing a single-step direct electrospinning process. These hybrid CA-PNIPAM membranes demonstrate the ability to switch between two wetting states viz. superhydrophilic to highly hydrophobic states upon increasing the temperature. At room temperature (23 °C) CA-PNIPAM nanofibrous membranes exhibit superhydrophilicity, while at elevated temperature (40 °C) the membranes demonstrate hydrophobicity with a static water contact angle greater than 130°. Furthermore, the results here demonstrate that the degree of hydrophobicity of the membranes can be controlled by adjusting the ratio of PNIPAM in the CA-PNIPAM mixture.
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Resinas Acrílicas/química , Celulosa/análogos & derivados , Celulosa/química , Nanofibras/química , Propiedades de Superficie , Temperatura , HumectabilidadRESUMEN
Our investigation of the structure-activity and structure-liability relationships for dihydropyrrolopyrazol-6-one MCHR1 antagonists revealed that off-rate characteristics, inferred from potencies in a FLIPR assay following a 2 h incubation, can impact in vivo efficacy. The in vitro and exposure profiles of dihydropyrrolopyrazol-6-ones 1b and 1e were comparable to that of the thienopyrimidinone counterparts 41 and 43 except for a much faster MCHR1 apparent off-rate. The greatly diminished dihydropyrrolopyrazol-6-one anti-obesity response may be the consequence of this rapid off-rate.
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Fármacos Antiobesidad/química , Pirazoles/química , Receptores de Somatostatina/antagonistas & inhibidores , Animales , Fármacos Antiobesidad/farmacocinética , Fármacos Antiobesidad/farmacología , Fármacos Antiobesidad/uso terapéutico , Semivida , Humanos , Obesidad/tratamiento farmacológico , Unión Proteica , Pirazoles/farmacocinética , Pirazoles/farmacología , Pirazoles/uso terapéutico , Ratas , Ratas Sprague-Dawley , Receptores de Somatostatina/metabolismo , Relación Estructura-Actividad , Pérdida de Peso/efectos de los fármacosRESUMEN
Nanotechnology refers to the fabrication, characterization, and application of substances in nanometer scale dimensions for various ends. The influence of nanotechnology on the healthcare industry is substantial, particularly in the areas of disease diagnosis and treatment. Recent investigations in nanotechnology for drug delivery and tissue engineering have delivered high-impact contributions in translational research, with associated pharmaceutical products and applications. Over the past decade, the synthesis of nanofibers or nanoparticles via electrostatic spinning or spraying, respectively, has emerged as an important nanostructuring methodology. This is due to both the versatility of the electrospinning/electrospraying process and the ensuing control of nanofiber/nanoparticle surface parameters. Electrosprayed nanoparticles and electrospun nanofibers are both employed as natural or synthetic carriers for the delivery of entrapped drugs, growth factors, health supplements, vitamins, and so on. The role of nanofiber/nanoparticle carriers is substantiated by the programmed, tailored, or targeted release of their contents in the guise of tissue engineering scaffolds or medical devices for drug delivery. This review focuses on the nanoformulation of natural materials via the electrospraying or electrospinning of nanoparticles or nanofibers for tissue engineering or drug delivery/pharmaceutical purposes. Here, we classify the natural materials with respect to their animal/plant origin and macrocyclic, small molecule or herbal active constituents, and further categorize the materials according to their proteinaceous or saccharide nature.
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Materiales Biocompatibles , Sistemas de Liberación de Medicamentos , Nanopartículas , Nanotecnología , Ingeniería de Tejidos , Animales , Células Cultivadas , Técnicas Electroquímicas , Humanos , Ratones , RatasRESUMEN
Topical application of antifungals does not have predictable or well-controlled release characteristics and requires reapplication to achieve therapeutic local concentration in a reasonable time period. In this article, the efficacy of five different US Food and Drug Administration-approved antifungal-loaded (amphotericin B, natamycin, terbinafine, fluconazole, and itraconazole) electrospun gelatin fiber mats were compared. Morphological studies show that incorporation of polyenes resulted in a two-fold increase in fiber diameter and the mats inhibit the growth of yeasts and filamentous fungal pathogens. Terbinafine-loaded mats were effective against three filamentous fungal species. Among the two azole antifungals compared, the itraconazole-loaded mat was potent against Aspergillus strains. However, activity loss was observed for fluconazole-loaded mats against all of the test organisms. The polyene-loaded mats displayed rapid candidacidal activities as well. Biophysical and rheological measurements indicate strong interactions between polyene antifungals and gelatin matrix. As a result, the polyenes stabilized the triple helical conformation of gelatin and the presence of gelatin decreased the hemolytic activity of polyenes. The polyene-loaded fiber mats were noncytotoxic to primary human corneal and sclera fibroblasts. The reduction of toxicity with complete retention of activity of the polyene antifungal-loaded gelatin fiber mats can provide new opportunities in the management of superficial skin infections.
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Antifúngicos/administración & dosificación , Antifúngicos/química , Preparaciones de Acción Retardada/química , Hongos/fisiología , Gelatina/química , Membranas Artificiales , Materiales Biocompatibles , Supervivencia Celular/efectos de los fármacos , Preparaciones de Acción Retardada/administración & dosificación , Difusión , Sinergismo Farmacológico , Galvanoplastia/métodos , Hongos/efectos de los fármacos , Nanocápsulas/administración & dosificación , Nanocápsulas/química , RotaciónRESUMEN
Drug-eluting medical implants are more common, particularly for fighting against cancers. FDA and other drug regulatory bodies have approved many nanoformulated devices eluting active pharmaceutical ingredients and thus there is growing demand for further value- added devices. Nanofibre membranes are known for its versatility of drug incorporation and sustained drug release. We intend to fabricate natural ingredient or extract, and their combination loaded polycaprolactone (PCL) nanofibre for usage as drug-eluting stents or implants for anticancer activity against lung and breast cancers. The fabricated nanofibre membranes were characterised by scanning electron microscope for morphology, FT-IR for chemical nature and tensile testing for mechanical strengths. Release of curcumin was studied with time to find the applicability of the device as drug-eluting implant. The activity of the nanofibre membranes was tested against human breast cancer (MCF7) and lung cancer (A459) cell lines in vitro. In both the cell lines tested, 1% aloe vera and 5% curcumin-loaded PCL nanofibre exhibited 15% more cytotoxicity in comparison with the commercial drug 1% cis-Platin-loaded PCL nanofibre after 24 h incubation.
Asunto(s)
Neoplasias de la Mama/patología , Curcumina/química , Portadores de Fármacos/química , Neoplasias Pulmonares/patología , Nanofibras/química , Extractos Vegetales/química , Antineoplásicos/química , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Neoplasias de la Mama/tratamiento farmacológico , Línea Celular Tumoral , Humanos , Neoplasias Pulmonares/tratamiento farmacológico , Poliésteres/químicaRESUMEN
Nanofibers play a significant role in tissue engineering and drug delivery because of the ease with which drugs or pharmaceuticals may be incorporated into the nano-formulation. Natural protein nanofibers are cross-linked (CXLed) employing a new protocol to improve their stability for perspective usage as tissue engineering or drug delivery scaffolds. The protocol utilizes a non-toxic, natural material vitamin based CXL protocol that works well for stabilizing protein nanofibers. We have tested the generation of reactive oxygen species (ROS) from UV treated riboflavin-gelatin microfibers, film or solution that helps in gelatin (Gel) CXLing and results in improved mechanical properties. Further natural proteins Gel and fibrinogen (Fib) solutions were also CXLed using vitamin B2 (riboflavin (Rib)) released from Rib-loaded polycaprolactone (PCL) nanofibers followed by UV treatment. The sustained release of Rib from PCL nanofibers is studied with in vitro drug release experiments and in vitro hydrogel formation upon treatment with the natural protein solutions. Rib-loaded nanofibers were characterized with SEM and AFM for morphology, mechanical strength calculation and FT-IR for ensuring drug incorporation. The Rib encapsulation in the nanofiber reservoirs enables the sustained release, and the ROS generating nanofibers could find application as a patch for CXLing any protein fiber or fibrous tissue, such as ocular, skin or cardiac tissue engineering.
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Heart disease is the leading cause of mortality in many industrialized nations and is often related to irregularities in electrical function that can radically damage cardiac functioning. The aim of this study is to develop a novel therapeutic hybrid scaffold that can couple electrical, mechanical, and biological properties, desirable for cardiac tissue regeneration. BSA/PVA scaffolds are fabricated in the ratio 2:1 and gold nanoparticles (AuNPs) embedded scaffolds in the ratios BSA/PVA/Au of 2:1:0.1 (lower concentration) and BSA/PVA/Au of 2:1:0.4 (higher concentration) by electrospinning. The scaffolds are characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), contact angle, Fourier transform infrared (FTIR) spectroscopy, and tensile testing to analyze the fiber morphology, AuNP distribution, hydrophilicity, surface functional groups, and mechanical properties of the scaffolds, respectively. Results show that ex vivo pretreatment of MSCs using 5-aza and AuNPs loaded conductive nanofibrous construct could lead to enhanced cardiomyogenic differentiation and result in superior biological and functional effects on infarcted myocardium regeneration.
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Diferenciación Celular , Miocardio/patología , Regeneración , Trasplante de Células Madre , Técnicas de Cultivo de Célula , Proliferación Celular/genética , Oro/administración & dosificación , Oro/química , Humanos , Nanopartículas del Metal/administración & dosificación , Nanopartículas del Metal/química , Microscopía Electrónica de Rastreo , Infarto del Miocardio/patología , Infarto del Miocardio/terapia , Nanofibras/administración & dosificación , Nanofibras/química , Células Madre/citología , Ingeniería de TejidosRESUMEN
Engineered nanofibers are generally focused on filtration, solar cells, sensors, smart textile fabrication, tissue engineering, etc. Electrospun nanofibers have potential advantages in tissue engineering and regenerative medicine, because of the ease in the incorporation of drugs, growth factors, natural materials, and inorganic nanoparticles in to these nanofiber scaffolds. Electrospun nanofiber scaffolds composed of synthetic and natural polymers are being explored as scaffolds similar to natural extracellular matrix for tissue engineering. The requirement of the inorganic composites in the nanofiber scaffolds for favouring hard and soft tissue engineering applications is dealt in detail in the present review. Regarding drug delivery applications of the composite nanofibers, the review emphasizes on wound healing with silver nanoparticles incorporated nanofibers, bone tissue engineering, and cancer chemotherapy with titanium and platinum complexes loaded nanofibers. The review also describes gold nanoparticle loaded nanofibers for cancer diagnosis and cosmetic applications.
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Materiales Biocompatibles/química , Nanofibras/química , Animales , Sistemas de Liberación de Medicamentos/métodos , Técnicas Electroquímicas/instrumentación , Técnicas Electroquímicas/métodos , Diseño de Equipo , Humanos , Nanofibras/ultraestructura , Medicina Regenerativa/métodos , Ingeniería de Tejidos/métodos , Cicatrización de HeridasRESUMEN
Nanotechnology based Pharma has emerged significantly and has influenced the Pharma industry up to a considerable extent. Nanoparticles technology holds a good share of the nanotech Pharma and is significant in comparison with the other domains. Electrospraying technology answers the potential needs of nanoparticle production such as scalability, reproducibility, effective encapsulation etc. Many drugs have been electrosprayed with and without polymer carriers. Drug release characteristics are improved with the incorporation of biodegradable polymer carriers which sustain the release of encapsulated drug. Electrospraying is acknowledged as an important technique for the preparation of nanoparticles with respect to pharmaceutical applications. Herein we attempted to consolidate the reports pertaining to electrospraying and their corresponding therapeutic application area.
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Sistemas de Liberación de Medicamentos/métodos , Electroquímica/métodos , Nanopartículas/administración & dosificación , Composición de Medicamentos/métodos , Humanos , Nanopartículas/química , Nanotecnología/métodos , Tecnología Farmacéutica/métodosRESUMEN
Biocompatible PCL polymer nanofiber mediated sustained release of hydrophilic drug and applicability as transdermal delivery system is attempted. This new attempt to investigate water soluble vitamin delivery with hydrophobic polymer nanofiber sustained the release of the vitamin and the method is suited for the transdermal patch applications. The drug loaded fibers were characterized with SEM for morphology, porometer for pore size measurements, mechanical strength calculation and FT-IR for drug load characterization. The contact angle measurement showed surface wettability and controlled release of drug was quantified with UV absorption measurements. To further enhance the release of vitamin, the polymer fiber was plasma treated at different time intervals and made hydrophilic gradually. Since the increased surface area and drug encapsulation in nano-reservoirs can able to release drug in small quantities and in a sustained manner we attempted the release of the energy supplement with nanofibrous delivery mode.
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Sistemas de Liberación de Medicamentos , Nanofibras/química , Poliésteres/química , Vitamina B 12/química , Complejo Vitamínico B/química , Suplementos Dietéticos , Microscopía Electrónica de Rastreo , Nanofibras/ultraestructura , Solubilidad , Parche Transdérmico , Agua/químicaRESUMEN
A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.
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Materiales Biomiméticos/síntesis química , Reactivos de Enlaces Cruzados/química , Células Madre Mesenquimatosas/efectos de los fármacos , Nanofibras/química , Ácido Fítico/química , Ingeniería de Tejidos , Azacitidina/farmacología , Biomarcadores/metabolismo , Materiales Biomiméticos/farmacología , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Técnicas Electroquímicas , Matriz Extracelular/química , Fibrinógeno/química , Gelatina/química , Hemoglobinas/química , Humanos , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Miocardio/citología , Nanofibras/ultraestructura , Antígenos Thy-1/metabolismo , Andamios del Tejido , Troponina/metabolismoRESUMEN
BACKGROUND: Heart failure due to myocardial infarction remains the leading cause of death worldwide owing to the inability of myocardial tissue regeneration. The aim of this study is to develop a core/shell fibrous cardiac patch having desirable mechanical properties and biocompatibility to engineer the infarcted myocardium. METHOD: We fabricated poly(glycerol sebacate)/fibrinogen (PGS/fibrinogen) core/shell fibers with core as elastomeric PGS provides suitable mechanical properties comparable to that of native tissue and shell as fibrinogen to promote cell-biomaterial interactions. The PGS/fibrinogen core/shell fibers and fibrinogen nanofibers were characterized by SEM, contact angle and tensile testing to analyze the fiber morphology, wettability, and mechanical properties of the scaffold. The cell-scaffold interactions were analyzed using isolated neonatal cardiomyocytes for cell proliferation, confocal analysis for the expression of marker proteins α-actinin, Troponin-T, ß-myosin heavy chain and connexin 43 and SEM analysis for cell morphology. RESULTS: We observed PGS/fibrinogen core/shell fibers had a Young's modulus of about 3.28 ± 1.7 MPa, which was comparable to that of native myocardium. Neonatal cardiomyocytes cultured on these scaffolds showed normal expression of cardiac specific marker proteins α-actinin, Troponin, ß-myosin heavy chain and connexin 43 to prove PGS/fibrinogen core/shell fibers have potential for cardiac tissue engineering. CONCLUSION: Results indicated that neonatal cardiomyocytes formed predominant gap junctions and expressed cardiac specific marker proteins on PGS/fibrinogen core/shell fibers compared to fibrinogen nanofibers, indicating PGS/fibrinogen core/shell fibers may serve as a suitable cardiac patch for the regeneration of infarcted myocardium.
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Decanoatos , Fibrinógeno , Glicerol/análogos & derivados , Proteínas de Microfilamentos/biosíntesis , Miocitos Cardíacos/metabolismo , Polímeros , Ingeniería de Tejidos/métodos , Andamios del Tejido , Actinina/biosíntesis , Animales , Animales Recién Nacidos , Proliferación Celular , Células Cultivadas , Decanoatos/química , Fibrinógeno/química , Regulación de la Expresión Génica , Glicerol/química , Polímeros/química , Ratas , Andamios del Tejido/química , Troponina T/biosíntesisRESUMEN
Myocardial tissue lacks the ability to appreciably regenerate itself following myocardial infarction (MI) which ultimately results in heart failure. Current therapies can only retard the progression of disease and hence tissue engineering strategies are required to facilitate the engineering of a suitable biomaterial to repair MI. The aim of this study was to investigate the in vitro properties of an injectable biomaterial for the regeneration of infarcted myocardium. Fabrication of core/shell fibers was by co-axial electrospinning, with poly(glycerol sebacate) (PGS) as core material and poly-L-lactic acid (PLLA) as shell material. The PLLA was removed by treatment of the PGS/PLLA core/shell fibers with DCM:hexane (2:1) to obtain PGS short fibers. These PGS short fibers offer the advantage of providing a minimally invasive injectable technique for the regeneration of infarcted myocardium. The scaffolds were characterized by SEM, FTIR and contact angle and cell-scaffold interactions using cardiomyocytes. The results showed that the cardiac marker proteins actinin, troponin, myosin heavy chain and connexin 43 were expressed more on short PGS fibers compared to PLLA nanofibers. We hypothesized that the injection of cells along with short PGS fibers would increase cell transplant retention and survival within the infarct, compared to the standard cell injection system.
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Decanoatos/química , Glicerol/análogos & derivados , Regeneración Tisular Dirigida/métodos , Miocitos Cardíacos/citología , Miocitos Cardíacos/fisiología , Nanoestructuras/química , Nanoestructuras/ultraestructura , Polímeros/química , Ingeniería de Tejidos/métodos , Animales , Proliferación Celular , Supervivencia Celular , Células Cultivadas , Cristalización/métodos , Glicerol/química , Inyecciones , Ensayo de Materiales , Procedimientos Quirúrgicos Mínimamente Invasivos , Tamaño de la Partícula , Conejos , Regeneración/fisiología , Propiedades de SuperficieRESUMEN
Tissue engineering scaffolds for skin tissue regeneration is an ever expounding area of research, as the products that meet the necessary requirements are far and elite. The nanofibrous poly-L-lactic acid/poly-(α,ß)-DL-aspartic acid/Collagen (PLLA/PAA/Col I&III) scaffolds were fabricated by electrospinning and characterized by SEM, contact angle and FTIR analysis for skin tissue regeneration. The cell-scaffold interactions were analyzed by cell proliferation and their morphology observed in SEM. The results showed that the cell proliferation was significantly increased (p≤0.05) in PLLA/PAA/Col I&III scaffolds compared to PLLA and PLLA/PAA nanofibrous scaffolds. The abundance and accessibility of adipose derived stem cells (ADSCs) may prove to be novel cell therapeutics for dermal tissue regeneration. The differentiation of ADSCs was confirmed using collagen expression and their morphology by CMFDA dye extrusion technique. The current study focuses on the application of PLLA/PAA/Col I&III nanofibrous scaffolds for skin tissue engineering and their potential use as substrate for the culture and differentiation of ADSCs. The objective for inclusion of a novel cell binding moiety like PAA was to replace damaged extracellular matrix and to guide new cells directly into the wound bed with enhanced proliferation and overall organization. This combinatorial epitome of PLLA/PAA/Col I&III nanofibrous scaffold with stem cell therapy to induce the necessary paracrine signalling effect would favour faster regeneration of the damaged skin tissues.
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Ácido Aspártico/química , Ácido Aspártico/farmacología , Colágeno/química , Colágeno/farmacología , Ácido Láctico/química , Ácido Láctico/farmacología , Nanofibras/química , Polímeros/química , Polímeros/farmacología , Animales , Proliferación Celular/efectos de los fármacos , Células Cultivadas , Matriz Extracelular/efectos de los fármacos , Poliésteres , Conejos , Regeneración/efectos de los fármacos , Piel/efectos de los fármacos , Ingeniería de Tejidos , Andamios del Tejido/químicaRESUMEN
A simple and economic synthesis of 3,4-dihydropyrimidin-2(1H)-ones using ammonium trifluoroacetate as catalyst and as solid support is accomplished. Easy workup procedure for the synthesis of title compounds is well arrived at and is well documented.
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In a SAR study, we have synthesized a few 1H-pyrazole carboxylate related microbicides using Vilsmeier reagent. The anti-microbial screening results of 1H-pyrazole-3-carboxylate are reported here for the first time. The effect of 1H-pyrazole carboxylates on the mycelial growth of plant pathogenic fungi is revealed. The first X-ray structure in the family of microbicidal 1H-pyrazole-4-carboxylates is presented.