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G3 (Bethesda) ; 6(10): 3017-3026, 2016 10 13.
Artículo en Inglés | MEDLINE | ID: mdl-27527793

RESUMEN

Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100 new LexA lines, including molecular mapping of insertions, description of enhancer-driven reporter expression in larval tissues, and adult neuroendocrine cells, comparison with established enhancer trap collections and tissue specific RNAseq. Generation of this open-resource LexA collection facilitates neuroendocrine and developmental biology investigations, and shows how empowering secondary school science can achieve research and educational goals.


Asunto(s)
Biología Evolutiva , Proteínas de Drosophila/genética , Drosophila/genética , Elementos de Facilitación Genéticos , Animales , Mapeo Cromosómico , Biología Evolutiva/métodos , Drosophila/metabolismo , Proteínas de Drosophila/metabolismo , Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Genes Reporteros , Inmunohistoquímica , Larva , Mutagénesis Insercional , Especificidad de Órganos/genética , Investigación
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