Intra- and inter-individual variability in the underwater pull-out technique in 200 m breaststroke turns.

The purpose of the present study was to investigate the intra- and inter-individual variability in arm-leg coordination during the underwater phase of the turn segment in 200 m breaststroke. Thirteen male swimmers were recruited and performed a 200 m breaststroke in a pre-calibrated 25 m pool. Sub-phases during the underwater segment were obtained using a notational analysis, and the mean velocity, displacement and duration during each sub-phase were obtained. A hierarchical cluster analysis (HCA) was performed using the analysed variables in all phases to identify inter-individual variability and random intra-individual variability. In addition, a linear mixed model (LMM: lap as a fixed effect and the participant as a random effect) was conducted to investigate systematic intra-individual variability. HCA identified three coordination patterns that were distinguished by the timing of the dolphin kick relative to the arm pull-out and the duration of the glide with arms at the side. All swimmers except one performed the arm pull-out after the dolphin kick. Nine swimmers maintained one coordination pattern, but other swimmers switched their coordination during the trial, particularly by shortening the duration of the glide with arms at the side. LMM showed a linear decrease (from the first to the last turn) in the time gap between the end of the dolphin kick and the start of the arm pull-out (a glide with the streamlined body position; F = 9.64, p = 0.034) and the glide duration with the arms at the side (F = 11.66, p = 0.015). In conclusion, both inter- and intra-individual variabilities during the underwater phase were evident in 200 m breaststroke turns, which were categorised into three patterns based on the timing of the dolphin kick and the duration of glides.

Antioxidant and Anti-Inflammatory Activity of Five Medicinal Mushrooms of the Genus Pleurotus.

Within the group of higher fungi, edible medicinal mushrooms have a long history of being used as food and in folk medicine. These species contain biologically active substances with many potential beneficial effects on human health. The Pleurotus genus is representative of medicinal mushrooms because Pleurotus ostreatus is one of the most commonly cultivated culinary mushrooms. In our study, we focused on lesser-known species in the genus Pleurotus and measured their antioxidant and anti-inflammatory activity. We prepared extracts of the mushrooms and analyzed them using HPLC-HRMS, GC-MS, and 1H-NMR. Significant differences in biological activities were found among the Pleurotus spp. extracts. A MeOH extract of P. flabellatus was the most active as a radical scavenger with the highest ORAC, while a chloroform extract had significant anti-inflammatory COX-2 activity. The 80% MeOH extract of P. flabellatus contained the highest amounts of ergosterol, ergothioneine, and mannitol. The 80% MeOH extract of P. ostreatus Florida was the most active in the NF-κB inhibition assay and had the highest content of ß-glucans (43.3% by dry weight). Given the antioxidant and anti-inflammatory properties of P. flabellatus, the potential therapeutic usefulness of this species is worth evaluating through in-depth investigations and confirmation by clinical trials.

Arm-leg coordination during the underwater pull-out sequence in the 50, 100 and 200 m breaststroke start.

OBJECTIVES: To investigate the arm-leg coordination from different perspectives of motor control during the underwater start sequence to understand whether differences exist between the three competitive breaststroke swimming events. DESIGN: Cross-sectional study. METHODS: Forty-one breaststroke races (with race times relative to the world record): 50-meter (n = 14, 87.6%), 100-meter (n = 14, 88.5%) and 200-meter (n = 13, 85.4%) were recorded. A race analysis system tracked the two-dimensional displacement of the head. Key points from the underwater start sequence were obtained from notational analysis in order to compute seven time-gaps and four phases to assess the arm-leg coordination and timing of the dolphin kick. A one-way ANOVA with Bonferroni post-hoc correction was used to assess differences between the time gaps and phases for the three events. RESULTS: Differences between the three events were found for total underwater glide, and the first (T0) and second (T1) major glide phase. No differences between the events were found in relative duration and distance for the time gaps related to arm-leg coordination (T1-3, T4, T6) and timing of the dolphin kick (T4-5) during the underwater start sequence. CONCLUSIONS: The arm-leg coordination and timing of the dolphin kick showed no difference between the events, but the total underwater glide duration was longer in both the 100- and 200-meter compared with the 50-meter start. This shows that swimmers did not change the complex inter-limb coordination between the competitive events, but only modified the least complex movement, gliding, to adapt to the swimming speed of the respective events.

Arm - Leg coordination profiling during the dolphin kick and the arm pull-out in elite breaststrokers.

In breaststroke races, the dolphin kick could finish before, at the same time, or during the arm pull-out, but it is unclear how swimmers perform this technique. The aim of this study was to investigate whether swimmers glide between the dolphin kick and arm pull-out, favour continuity or even overlap those two phases, as it would impact the active underwater sequence. Fourteen international and national male swimmers performed 100-m breaststroke with all-out effort in a pre-calibrated 25 m swimming pool. A multi-camera system tracked the head of the swimmers. Key points of the active underwater sequence were obtained from notational analysis. A hierarchical cluster analysis identified three coordination profiles. All swimmers started their dolphin kick before the arm pull-out. However, one swimmer started the arm pull-out before the end of the dolphin kick, seven swimmers started the arm pull-out after the end of the dolphin kick, and four swimmers synchronised the beginning of the arm pull-out and the end of the dolphin kick, while two other swimmers mixed two coordination profiles among the start and the three turns. Those different profiles allow achieving similar performance outcome, suggesting individual training regarding the underwater phase.

Symmetry breaking of the cellular lobes closely relates to phylogenetic structure within green microalgae of the Micrasterias lineage (Zygnematophyceae).

Green microalgae of the Micrasterias lineage are unicellular microorganisms with modular morphology consisting of successively differentiated lobes. Due to their morphological diversity and peculiar morphogenesis, these species are important model systems for studies of cytomorphogenesis and cellular plasticity. Interestingly, the phylogenetic structure of the Micrasterias lineage and most other Desmidiales is poorly related to the traditional morphological characters used for delimitation of taxa. In this study, we focused on symmetry breaking between adjacent cellular lobes in relation to phylogeny of the studied species. While pronounced morphological asymmetry between the adjacent lobes is typical for some species, others have been characterized by the almost identical morphologies of these structures. We asked whether there is any detectable average shape asymmetry between the pairs of lobes and terminal lobules in 19 Micrasterias species representing all major clades of this desmidiacean lineage. Then, we evaluated whether the asymmetric patterns among species are phylogenetically structured. The analyses showed that the phylogeny was in fact strongly related to the patterns of morphological asymmetry between the adjacent cellular lobes. Thus, evolution of the asymmetric development between the adjacent lobes proved to be the key event differentiating cellular shape patterns of Micrasterias. Conversely, the phylogeny was only weakly related to asymmetry between the pairs of terminal lobules. The subsequent analyses of the phylogenetic morphological integration showed that individual hierarchical levels of cellular morphology were only weakly coordinated with regard to asymmetric variation among species. This finding indicates that evolutionary differentiation of morphogenetic processes leading to symmetry breaking may be relatively independent at different branching levels. Such modularity is probably the key to the evolvability of cellular shapes, leading to the extraordinary morphological diversity of these intriguing microalgae.

Polyphasic evaluation of Xanthidium antilopaeum and Xanthidium cristatum (Zygnematophyceae, Streptophyta) species complex.

We investigated twenty-six strains of Xanthidium antilopaeum Kütz. and seven strains of X. cristatum Ralfs isolated from various European localities or obtained from public culture collections. A combination of molecular, geometric morphometric, and morphological data were used to reveal the patterns of the phylogenetic and morphological differentiation of these taxonomically very compli-cated desmid taxa. The molecular data based on trnG(ucc) and ITS rDNA sequences illustrated the monophyly of both the complexes, which indicated that their traditional morphology-based discriminative criteria, such as the different number of spines, may generally continue to be considered relevant. The single exception was X. antilopaeum var. basiornatum B. Eichler et Raciborski, which was positioned outside the X. antilopaeum/cristatum clade. The independent status of this taxon was also confirmed on the basis of the geometric morphometric data, so that we concluded that it probably represents a separate species. Within X. cristatum complex, the traditional varieties X. cristatum var. cristatum Ralfs, X. cristatum var. uncinatum Ralfs, and X. cristatum var. scrobiculatum Scott et Grönblad turned out to be separate taxa. Conversely, X. cristatum var. bituberculatum Lowe lacked any taxonomical value. Our data on X. antilopaeum illustrated extensive phylogenetic as well as phenotypic variability within this species complex. However, our data did not result in any unambiguous pattern that would allow sound taxonomic classification. Finally, we also found out that the morphologically peculiar Staurastrum tumidum Ralfs belongs to the genus Xanthidium based on the combined rbcL + cox III data set. Consequently, this species was formally transferred to this genus.