Asunto(s)
Tecnología Biomédica/métodos , Medicalización , Atención Prenatal , Ensayos Clínicos como Asunto , Femenino , Humanos , EmbarazoRESUMEN
Bacterial strains isolated from a large variety of necropsy samples of pigs and previously described as a phenotypical homogeneous group were shown to belong to the species Actinomyces hyovaginalis. This was unexpected because their colonial characteristics, as well as their origins, were very different from those originally reported for the vaginal strains on which the species description of A. hyovaginalis was based. Colonial morphology, as well as fermentation of cellobiose, reactions in hippurate and nitrate and production of beta-glucuronidase, allowed separation of the strains studied here from the vaginal strains. Analysis of tRNA intergenic length polymorphisms (tDNA-PCR), 16S rRNA-gene sequencing and DNA-DNA hybridizations were carried out and led to the proposal of a separate biotype within the species A. hyovaginalis. Since, the strains were isolated from different body sites, this biotype has been designated as the 'general' biotype of A. hyovaginalis, while the strains on which the original species description was based are designated as the 'vaginal' biotype.
Asunto(s)
Actinomyces/clasificación , Actinomicosis/veterinaria , Técnicas de Tipificación Bacteriana/veterinaria , ARN Ribosómico 16S/análisis , Enfermedades de los Porcinos/diagnóstico , Enfermedades Vaginales/veterinaria , Actinomyces/genética , Actinomicosis/diagnóstico , Actinomicosis/microbiología , Animales , Técnicas de Tipificación Bacteriana/métodos , Secuencia de Bases , ADN Bacteriano/química , ADN Ribosómico/genética , Femenino , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Fenotipo , Filogenia , ARN de Transferencia/genética , Homología de Secuencia de Ácido Nucleico , Porcinos , Enfermedades de los Porcinos/microbiología , Enfermedades Vaginales/diagnóstico , Enfermedades Vaginales/microbiologíaRESUMEN
The discriminatory power, speed, and interlaboratory reproducibility of tRNA intergenic length polymorphism analysis (tDNA-PCR) combined with capillary electrophoresis was evaluated for the identification of streptococci. This method was carried out in three different laboratories under highly standardized conditions for 54 strains belonging to 18 different species. It was concluded that interlaboratory reproducibility of tDNA fingerprints produced by means of capillary electrophoresis was sufficiently high to permit the exchange between different laboratories and the construction of common libraries which can be consulted for comparison with fingerprints obtained independently in separate laboratories. In a second step, 17 other species were included in the study and examined in one of the participating laboratories. All Streptococcus species studied, except S. mitis, S. oralis, S. parasanguinis, S. pneumoniae, S. thermophilus, and S. vestibularis, showed distinguishable tDNA fingerprints. A database of well-characterized strains was constructed to enable computer-aided identification of unknown streptococcal isolates.
Asunto(s)
ADN Intergénico/genética , Reacción en Cadena de la Polimerasa/normas , Polimorfismo Genético/genética , ARN de Transferencia/genética , Streptococcus/clasificación , Dermatoglifia del ADN/normas , ADN Bacteriano/genética , Bases de Datos Factuales , Electroforesis Capilar/métodos , Electroforesis Capilar/normas , Humanos , Laboratorios/normas , Reacción en Cadena de la Polimerasa/métodos , Reproducibilidad de los Resultados , Programas Informáticos , Infecciones Estreptocócicas/diagnóstico , Streptococcus/genéticaRESUMEN
tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR enabled us to discriminate for all species tested. Enterococcus faecium showed minor but reproducible differences with Enterococcus durans, while Enterococcus hirae was easily distinguishable. Enterococcus avium, Enterococcus malodoratus, and Enterococcus raffinosus generated highly similar though distinctive patterns.