RESUMEN
Circular RNAs (circRNAs) are abundant and evolutionarily conserved RNAs of largely unknown function. Here, we show that a subset of circRNAs is translated in vivo. By performing ribosome footprinting from fly heads, we demonstrate that a group of circRNAs is associated with translating ribosomes. Many of these ribo-circRNAs use the start codon of the hosting mRNA, are bound by membrane-associated ribosomes, and have evolutionarily conserved termination codons. In addition, we found that a circRNA generated from the muscleblind locus encodes a protein, which we detected in fly head extracts by mass spectrometry. Next, by performing in vivo and in vitro translation assays, we show that UTRs of ribo-circRNAs (cUTRs) allow cap-independent translation. Moreover, we found that starvation and FOXO likely regulate the translation of a circMbl isoform. Altogether, our study provides strong evidence for translation of circRNAs, revealing the existence of an unexplored layer of gene activity.
Asunto(s)
Proteínas de Drosophila/biosíntesis , Drosophila melanogaster/metabolismo , Proteínas Nucleares/biosíntesis , Biosíntesis de Proteínas , ARN/metabolismo , Ribosomas/metabolismo , Animales , Línea Celular , Codón Iniciador , Codón de Terminación , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Factores de Transcripción Forkhead/metabolismo , Genotipo , Cabeza , Espectrometría de Masas , Ratones , Mutación , Proteínas Nucleares/genética , Conformación de Ácido Nucleico , Estado Nutricional , Fenotipo , ARN/química , ARN/genética , Caperuzas de ARN/química , Caperuzas de ARN/genética , ARN Circular , Ratas , Ribosomas/química , Ribosomas/genética , Inanición/genética , Inanición/metabolismo , Relación Estructura-Actividad , TransfecciónRESUMEN
Circular RNAs (circRNAs) are an endogenous class of animal RNAs. Despite their abundance, their function and expression in the nervous system are unknown. Therefore, we sequenced RNA from different brain regions, primary neurons, isolated synapses, as well as during neuronal differentiation. Using these and other available data, we discovered and analyzed thousands of neuronal human and mouse circRNAs. circRNAs were extraordinarily enriched in the mammalian brain, well conserved in sequence, often expressed as circRNAs in both human and mouse, and sometimes even detected in Drosophila brains. circRNAs were overall upregulated during neuronal differentiation, highly enriched in synapses, and often differentially expressed compared to their mRNA isoforms. circRNA expression correlated negatively with expression of the RNA-editing enzyme ADAR1. Knockdown of ADAR1 induced elevated circRNA expression. Together, we provide a circRNA brain expression atlas and evidence for important circRNA functions and values as biomarkers.
