1.
Bioorg Med Chem Lett
; 10(8): 801-4, 2000 Apr 17.
Artículo
en Inglés
| MEDLINE
| ID: mdl-10782690
RESUMEN
Incorporation of a 4-hydroxy-N-acetylprolinol nucleotide analogue at the 3'-terminus of DNA or 2-5A-DNA sequences resulted in a significantly enhanced 3'-exonuclease resistance while the affinity for complementary RNA was only slightly decreased. Furthermore, the binding to and activation of human RNase L by thus modified 2-5A-DNA conjugates was not altered as compared to the parent unmodified 2-5A-DNAs.
Asunto(s)
Nucleótidos de Adenina/química , ADN sin Sentido/química , Exonucleasas/metabolismo , Nucleótidos/química , Oligorribonucleótidos/química , Secuencia de Bases , Endorribonucleasas/metabolismo , Activación Enzimática , Humanos , ARN Mensajero/genética
2.
Nucleosides Nucleotides
; 18(6-7): 1339-41, 1999.
Artículo
en Inglés
| MEDLINE
| ID: mdl-10474222
RESUMEN
Three new cholesterol-containing phosphoramidites where synthesized and used in automated synthesis of modified DNA fragments. These cholesterol lesions are good substrates for the E. coli UvrABC endonuclease. In vitro they are incised from damaged DNA with higher efficiency in respect with the cholesterol lesions previously published.