Full-resolution image restoration for light field images via a spatial shift-variant degradation network.

The light field (LF) imaging systems face a trade-off between the spatial and angular resolution in a limited sensor resolution. Various networks have been proposed to enhance the spatial resolution of the sub-aperture image (SAI). However, the spatial shift-variant characteristics of the LF are not considered, and few efforts have been made to recover a full-resolution (FR) image. In this paper, we propose an FR image restoration method by embedding LF degradation kernels into the network. An explicit convolution model based on the scalar diffraction theory is first derived to calculate the system response and imaging matrix. Based on the analysis of LF image formation, we establish the mapping from an FR image to the SAI through the SAI kernel, which is a spatial shift-variant degradation (SSVD) kernel. Then, the SSVD kernels are embedded into the proposed network as prior knowledge. An SSVD convolution layer is specially designed to handle the view-wise degradation feature and speed up the training process. A refinement block is designed to preserve the entire image details. Moreover, our network is evaluated on extensive simulated and real-world LF images to demonstrate its superior performance compared with other methods. Experiments on a multi-focus scene further prove that our network is suitable for any in-focus or defocused conditions.

Juvenile Hormone Involved in the Defensive Behaviors of Soldiers in Termite Reticulitermes aculabialis.

Eusocial insects have evolved specific defensive strategies to protect their colonies. In termite colonies, soldiers perform a colony-level defense by displaying mechanical biting, head-banging and mandible opening-closing behaviors. However, few studies have been reported on the factors modulating defensive behaviors in termites. Owing to JH (juvenile hormone) being involved in soldier differentiation, JH was speculated to affect defensive behaviors in termite soldiers. To determine the effect of JH on the defensive behaviors of termite soldiers, we performed a JHA-feeding and RaSsp1-silencing experiment and then tested the changes in defense-related behaviors, alarm pheromones and key JH signaling genes. The observed result was that after feeding workers with JHA, soldiers displayed the following: (1) decreased biting events and increased head-banging events; (2) a reduced expression of RaSsp1 and increased expression of Met (methoprene-tolerant, the nuclear receptor of JH) and Kr-h1 (the JH-inducible transcription factor Krüppel homolog 1); and (3) a decreased concentration of alarm pheromones, including α-pinene, ß-pinene and limonene (+, -). Further study showed that soldiers silenced for RaSsp1 also exhibited (1) decreased biting events and increased head-banging events and (2) increased expression of Met and Kr-h1. In addition, soldiers stimulated by the alarm pheromone limonene displayed an increase in the frequency of mandible opening-closing and biting behavior. All of these results show that JHA influenced the defensive behaviors of termite soldiers, possibly via downregulating RaSsp1 expression, up-regulating Met and Kr-h1 and stimulating the secretion of alarm pheromones, suggesting that the JH pathway plays important roles in modulating social behaviors in termite colonies.

RETRACTED ARTICLE: Analysis of fractured soft rock characteristics in fault rupture zones and laneway shoring.

Fault rupture is a common phenomenon in geotechnical engineering. To prevent rupture, laneway shoring is performed, prior to which, convergence deformation, failure criteria, and fracture development in soft rocks in the fault rupture zone are carefully analyzed. Then, a supporting structure corresponding to the actual situation of the soft rock in the rupture zone is created. Herein, the water-rich laneway shoring through the fault rupture zone of the Hongqingliang coal mine located in the Inner Mongolia Autonomous Region is taken as the research object. Then, the fracture development and characteristics of argillaceous siltstones and laneway shoring cross-fault rupture zone are studied. Site inspection, indoor and field tests, theoretical analysis, numerical simulation, and field monitoring were used for systematic fracture analysis. Results indicated that laneway shoring through the fault fracture zone in the Hongqingliang coal mine could help prevent disasters. This method was extended to laneway supports built through the fault rupture zones in mines in other areas of China.

The role of cell death in SARS-CoV-2 infection.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), showing high infectiousness, resulted in an ongoing pandemic termed coronavirus disease 2019 (COVID-19). COVID-19 cases often experience acute respiratory distress syndrome, which has caused millions of deaths. Apart from triggering inflammatory and immune responses, many viral infections can cause programmed cell death in infected cells. Cell death mechanisms have a vital role in maintaining a suitable environment to achieve normal cell functionality. Nonetheless, these processes are dysregulated, potentially contributing to disease pathogenesis. Over the past decades, multiple cell death pathways are becoming better understood. Growing evidence suggests that the induction of cell death by the coronavirus may significantly contributes to viral infection and pathogenicity. However, the interaction of SARS-CoV-2 with cell death, together with its associated mechanisms, is yet to be elucidated. In this review, we summarize the existing evidence concerning the molecular modulation of cell death in SARS-CoV-2 infection as well as viral-host interactions, which may shed new light on antiviral therapy against SARS-CoV-2.

Decreasing hydrophobicity or shielding hydrophobic areas of CH2 attenuates low pH-induced IgG4 aggregation.

Protein aggregation is a major challenge in the development of therapeutic monoclonal antibodies (mAbs). Several stressors can cause protein aggregation, including temperature shifts, mechanical forces, freezing-thawing cycles, oxidants, reductants, and extreme pH. When antibodies are exposed to low pH conditions, aggregation increases dramatically. However, low pH treatment is widely used in protein A affinity chromatography and low pH viral inactivation procedures. In the development of an IgG4 subclass antibody, mAb1-IgG4 showed a strong tendency to aggregate when temporarily exposed to low pH conditions. Our findings showed that the aggregation of mAb1-IgG4 under low pH conditions is determined by the stability of the Fc. The CH2 domain is the least stable domain in mAb1-IgG4. The L309E, Q311D, and Q311E mutations in the CH2 domain significantly reduced the aggregation propensity, which could be attributed to a reduction in the hydrophobicity of the CH2 domain. Protein stabilizers, such as sucrose and mannose, could also attenuate low pH-induced mAb1-IgG4 aggregation by shielding hydrophobic areas and increasing protein stability. Our findings provide valuable strategies for managing the aggregation of protein therapeutics with a human IgG4 backbone.

Synergetic benefits of pollution and carbon reduction from fly ash resource utilization-Based on the life cycle perspective.

Synergetic reduction of pollution and carbon is a key strategy for the fundamental improvement of ecological and environmental quality and carbon neutrality. Solid waste resource utilization can reduce the secondary pollution caused by conventional solid waste disposal and presents evident environmental potential. Based on the comparable system boundaries, this study adopts life cycle assessment (LCA) to compare the environmental impact of three fly ash utilization paths. The synergy index is then defined according to six environmental impact indicators to quantitatively evaluate the synergetic effects of carbon emission and pollutant reduction. The results confirm the possibility of fly ash resource utilization for synergetic pollution and carbon reduction and demonstrate the synergetic emission reduction potential of the solid waste resource utilization supply chain. This study is both an application of the life cycle assessment model in the solid waste utilization and disposal field and provides insight into the pathway of pollution and carbon reduction in China.

Analysis of regional differences and spatial spillover effects of agricultural carbon emissions in China.

In order to realize "double carbon" target in agriculture and high-quality development of the rural economy in China, it is crucial to study the regional differences and spatial spillover effects of agricultural carbon emissions (ACE). This paper measures ACE using panel data of 31 Chinese provinces from 2005 to 2020, examines the spatio-temporal evolution characteristics，the convergence of agricultural carbon emissions, compares and analyzes regional differences, and investigates the spatial correlation and spatial spillover effects. The study found that: (1) Total agricultural carbon emissions over the research period exhibit a rising and then reducing trend, the spatial distribution of total agricultural carbon emissions is described as high in east-central and low in west. The gap of agricultural carbon emissions is gradually declining in the east, and will eventually reach their respective steady-state levels in the west and northeast. (2) There is a strong spatial interprovincial link of ACE, which has a beneficial knock-on effect on the convergence of adjacent provinces. (3) Agricultural industrial structure, urbanization level, the size of the agricultural labor force, and the intensity of the agricultural machinery input all directly affect ACE in this province and indirectly affect ACE in adjacent provinces, with the exception of the negligible coefficient of economic development level on ACE. Hence, pertinent policy suggestions are put out to serve as a guide for reducing ACE.

Transcriptomics Reveals the Killing Mechanism by Which Entomopathogenic Fungi Manipulate the RNA Expression Profiles of Termites and Provides Inspiration for Green Pest Management.

As chemical pesticides have caused serious environmental pollution, fungus-based biological control has become a developing alternative to chemical control. Here, we aimed to determine the molecular mechanism underlying how Metarhizium anisopliae facilitated invasive infection. We found that the fungus increased its virulence by downregulating glutathione S-transferase (GST) and superoxide dismutase (SOD) throughout termite bodies. Among 13 fungus-induced microRNAs throughout termite bodies, miR-7885-5p and miR-252b upregulation significantly downregulated several mRNAs in response to toxic substances to increase the fungal virulence [e.g., phosphoenolpyruvate carboxykinase (GTP) and heat shock protein homologue SSE1]. In addition, nanodelivered small interfering RNA of GST and SOD and miR-7885-5p and miR-252b mimics increased the virulence of the fungus. These findings provide new insights into the killing mechanism of entomopathogens and their utilization of the host miRNA machinery to reduce host defenses, laying the groundwork to enhance virulence of biocontrol agents for green pest management.

Effects of Schisandra chinensis Polysaccharide-Conjugated Selenium Nanoparticles on Intestinal Injury in Mice.

Schisandra chinensis polysaccharide (SCP) is an experimental therapeutic for the treatment of intestinal injury. Selenium nanoparticle modification can improve the bioactivity of polysaccharides. In this study, SCP was firstly extracted and purified by a DEAE-52 column, then SCP-Selenium nanoparticles (SCP-Se NPs) were prepared, and the procedure was optimized. Thereafter, the obtained SCP-Se NPs were characterized by transmission electron microscope, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The influence of different storage environments on the stability of colloidal SCP-Se NPs was also investigated. Finally, the therapeutic effects of SCP-Se NPs on LPS-induced intestinal inflammatory injuries in mice were evaluated. Results showed that the optimized SCP-Se NPs were amorphous, uniform, spherical particles with a diameter of 121 nm, and the colloidal solution was stable at 4 °C for at least 14 d. Moreover, SCP-Se NPs could more effectively alleviate LPS-induced diarrhea, intestinal tissue injury, and tight junction destruction and decrease the elevated expression levels of TNF-α, IL-1ß, and IL-6 compared with SCP. These results demonstrate that SCP-Se NPs may alleviate LPS-induced enteritis through their anti-inflammatory effects, indicating that SCP-Se NPs can serve as a good candidate for preventing and treating enteritis in the livestock and poultry industry.

Study on Mechanical Properties and Micro Characterization of Fibre Reinforced Ecological Cementitious Coal Gangue Materials.

Eco-gelled coal gangue materials (EGCGMs) are usually produced using coal gangue, slag, and fly ash in a highly alkaline environment. Herein, to improve the mechanical properties of such materials, polypropylene fibers were uniformly mixed with them. An unconfined compressive strength test and a three-point bending test of the fiber-reinforced EGCGMs under different conditions were conducted. Based on the performance degradation control technology of the fiber structure, the interface mechanism of the composite materials was analyzed from the micro level using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). In the mechanical test, the 28 d UCS and flexural properties of the fiber-reinforced EGCGMs were analyzed using the Box-Behnken design response surface design method and orthogonal design method, respectively. The order of significance was as follows: sodium hydroxide, fiber length, and fiber content. Within the scope of the experimental study, when the NaOH content is 3, the fiber content is 5 ‱, and the fiber length is 9 mm, the mechanical properties are the best. Based on the microscopic equipment, it was discovered that the amorphous ecological glue condensation product formed by the reaction of slag and fly ash in the alkaline environment was filled between the coal gangue particles and the fibers, and several polymerization products accumulated to form a honeycomb network topology. The distribution of fibers in the EGCGM matrix could be primarily divided into single embedded and network occurrences. The fiber inhibits the crack initiation and development of the matrix through the crack resistance effect, and improves the brittleness characteristics through the bridging effect during the failure process, which promotes the ductility of the ecological cementitious coal gangue matrix.The results presented herein can provide a theoretical basis for improving the mechanical properties of alkali-activated geopolymers.

Developmental Features, Influencing Factors, and Formation Mechanism of Underground Mining-Induced Ground Fissure Disasters in China: A Review.

Mining-induced ground fissures are one of the major geological disasters affecting coal mines. In recent years, many effective monitoring methods have been developed to explore the developmental characteristics and nature of mining-induced ground fissures for being treated scientifically. This paper is mainly on the development law and mechanism of mining ground fissure research results which have been comprehensively combed, highlighting the development trend, including the formation condition, development features, influencing factors, and mechanical mechanism of mining-induced ground fissures. Outstanding issues are discussed and future research hot spots and trends are pointed out. The major conclusions include: (1) under the shallow coal mining condition, because the rock layer fault zone directly reaches the surface, the ground fissure usually develops seriously; (2) mining-induced ground fissures are generally divided into four types: tensile fissures, compression fissures, collapsed fissures, and sliding fissures; (3) mining-induced ground fissures are affected by the coupling effect of underground mining and surface topography. The main factors are geological mining conditions, surface deformation, and surface topography, including rock and soil structure, rock and soil mechanical properties, surface horizontal deformation, surface slope, and so on; and (4) to ensure the safety of underground mining, temporary ground fissures formed during the process of coal mining must be treated when ground fissures and rock ground fissures are connected. The results of this article make up for the deficiencies of the relevant research, provide the basis and direction for future research, and have universal applicability and scientific guiding significance.

Technology innovations impact on carbon emission in Chinese cities: exploring the mediating role of economic growth and industrial structure transformation.

China seems ambitious to achieve a "carbon emissions peak" before 2030 and "carbon neutrality" before 2060. To deal with this emissions mitigation plan, technology innovations are regarded as a crucial factor. However, considering its rebound effect (CO2 emissions driving effect) through economic growth, technology innovations might not prove a promising contributor to CO2 reduction. Therefore, there is a need to investigate further the nexus between technology innovations and CO2 emissions for conclusive debate. Based on the data of 215 cities in China, this paper uses mediating effects model to investigate the direct and indirect impacts (through economic growth and industrial structure transformation) of technology innovations on CO2 emissions from a microeconomic perspective. The main results suggest that technology innovations generally increase CO2 emissions in China both directly and indirectly. The impact of technology innovations and economic growth on CO2 emissions indicated the EKC characteristics. Furthermore, the contributions of technology innovations to CO2 emissions are distinguished in different regions. Thus, there is an urgent need for China to promote innovations in "clean technology" and to transform industrial structure to the tertiary industry to achieve the targets of carbon neutrality and emissions peaking.

Progress of cGAS-STING signaling in response to SARS-CoV-2 infection.

Coronavirus disease 2019 (COVID-19) is an epidemic respiratory disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can cause infections in millions of individuals, who can develop lung injury, organ failure, and subsequent death. As the first line of host defense, the innate immune system is involved in initiating the immune response to SARS-CoV-2 infection and the hyperinflammatory phenotype of COVID-19. However, the interplay between SARS-CoV-2 and host innate immunity is not yet well understood. It had become known that the cGAS-STING pathway is involved in the detection of cytosolic DNA, which elicits an innate immune response involving a robust type I interferon response against viral and bacterial infections. Nevertheless, several lines of evidence indicate that SARS-CoV-2, a single-stranded positive-sense RNA virus, triggered the cGAS-STING signaling pathway. Therefore, understanding the molecular and cellular details of cGAS-STING signaling upon SARS-CoV-2 infection is of considerable biomedical importance. In this review, we discuss the role of cGAS-STING signaling in SARS-CoV-2 infection and summarize the potential therapeutics of STING agonists as virus vaccine adjuvants.

Comparison of risk of peritoneal dialysis-associated peritonitis between roxadustat and recombinant human erythropoietin in peritoneal dialysis patients: a retrospective comparative cohort study.

Background: Roxadustat and recombinant human erythropoietin (rhuEPO) have been approved for the treatment of renal anemia in patients undergoing dialysis. The comparison of risk of peritoneal dialysis (PD)-associated peritonitis between roxadustat and rhuEPO in PD patients remains uncertain. We aimed to compare the risk of PD-associated peritonitis between roxadustat and rhuEPO and examine possible modifiers for the comparison in PD patients. Methods: A total of 437 PD patients with renal anemia (defined as hemoglobin ≤10.0 g/dL) from 4 centers were selected. Participants were scheduled for follow-up every 1-3 months at each center. We compared differences in baseline characteristics by medication group and 1:1 matching group based on propensity scores. PD-associated peritonitis was defined according to the International Society for Peritoneal Dialysis guidelines. Univariable and multivariable Cox proportional hazard analyses were performed to compare the risk of PD-associated peritonitis between roxadustat and rhuEPO in PD patients. Propensity score matching method was used to examine the robustness of results. Results: A total of 437 participants, including 291 in roxadustat group and 146 in rhuEPO group, were included in the current study, respectively. During a median follow-up of 13.0 (25th-75th, 10.0-15.0) months, PD-associated peritonitis occurred in 68 patients, including 26 of 291 (0.10 episodes per patient-year) patients in the roxadustat group and 42 of 146 (0.27 episodes per patient-year) patients in the rhuEPO group. Overall, compared to patients in the rhuEPO group, the roxadustat group (hazard ratio, 0.345; 95% confidence interval: 0.202-0.589) was associated with a lower risk of PD-associated peritonitis with adjustment of use of roxadustat medication, age, sex, hypertension status, diabetes status, dialysis vintage, serum potassium, hemoglobin, and albumin. Furthermore, the results were consistent with the propensity score analysis. None of the variables, including age, sex, body mass index, PD vintage, presence of residual renal function, hemoglobin, albumin, serum potassium, and C-reactive protein levels, significantly modified the associations. Conclusions: Our study demonstrated that compared with rhuEPO, roxadustat may reduce the risk of PD-associated peritonitis in PD patients, highlighting the importance of roxadustat for the prevention of PD-associated peritonitis in PD patients.

Blockade of prostaglandin E2 receptor 4 ameliorates peritoneal dialysis-associated peritoneal fibrosis.

Inflammatory responses in the peritoneum contribute to peritoneal dialysis (PD)-associated peritoneal fibrosis. Results of our previous study showed that increased microsomal prostaglandin E synthase-1-mediated production of prostaglandin E2 (PGE2) contributed to peritoneal fibrosis. However, the role of its downstream receptor in the progression of peritoneal fibrosis has not been established. Here, we examined the role of PGE2 receptor 4 (EP4) in the development of peritoneal fibrosis. EP4 was significantly upregulated in peritoneal tissues of PD patients with ultrafiltration failure, along with the presence of an enhanced inflammatory response. In vitro experiments showed that exposure to high glucose concentrations enhanced EP4 expression in rat peritoneal mesothelial cells (RPMCs). High-glucose-induced expression of inflammatory cytokines (monocyte chemoattractant protein-1, tumour necrosis factor α, and interleukin 1ß) was significantly reduced in RPMCs treated with ONO-AE3-208, an EP4 receptor antagonist. ONO-AE3-208 also significantly decreased the expression of extracellular matrix proteins induced by high glucose concentrations. Furthermore, ONO-AE3-208 blunted activation of the NLR family pyrin domain containing 3 (NLRP3) inflammasome and phosphorylation of nuclear factor kappa B (NF-κB) (p-p65). To further investigate the functional role of EP4, ONO-AE3-208 was administrated for 4 weeks in a rat model of PD, the results of which showed that ONO-AE3-208 inhibited peritoneal fibrosis and improved peritoneal dysfunction. Additionally, inflammatory cytokines in the peritoneum of PD rats treated with ONO-AE3-208 were downregulated, in line with inhibition of the NLRP3 inflammasome and NF-κB phosphorylation. In conclusion, an EP4 antagonist reduced the development of peritoneal fibrosis, possibly by suppressing NLRP3 inflammasome- and p-p65-mediated inflammatory responses. Our findings suggest that an EP4 antagonist may be therapeutically beneficial for PD-associated peritoneal fibrosis.

Hybrid non-sequential modeling of an image mapping spectrometer.

An image mapping spectrometer (IMS) is a kind of snapshot imaging spectrometer characterized by containing several array components including the image mapper, prism array, and reimaging lens array. We propose a hybrid non-sequential modeling method of IMS and present the complete optical model of the system built in Zemax. This method utilizes the spatial periodicity of the array components and requires only a small number of input parameters. Moreover, we design a collimating lens of a large relative aperture, sufficient working distance, and low aberration to meet the requirements of an IMS with good optical performance and compact volume. The designed lens is quantitatively evaluated in the entire IMS model, and the results demonstrate that the lens has excellent optical performance. The evaluation on the collimating lens also demonstrates the capability of the proposed modeling method in the design and optimization of systems such as the IMS that contain multiple array components. The designed collimating lens is manufactured and assembled in the experimental setup of the IMS. The proposed modeling method is verified by experimental results.

Biodegradation of Gramineous Lignocellulose by Locusta migratoria manilensis (Orthoptera: Acridoidea).

Exploring an efficient and green pretreatment method is an important prerequisite for the development of biorefinery. It is well known that locusts can degrade gramineous lignocellulose efficiently. Locusts can be used as a potential resource for studying plant cell wall degradation, but there are few relative studies about locusts so far. Herein, some new discoveries were revealed about elucidating the process of biodegradation of gramineous lignocellulose in Locusta migratoria manilensis. The enzyme activity related to lignocellulose degradation and the content of cellulose, hemicellulose, and lignin in the different gut segments of locusts fed corn leaves were measured in this study. A series of characterization analyses were conducted on corn leaves and locust feces, which included field emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction pattern (XRD), and thermogravimetric (TG) analysis. These results showed that the highest activities of carboxymethyl cellulase (CMCase), filter paper cellulase (FPA), and xylanase were obtained in the foregut of locusts, which strongly indicated that the foregut was the main lignocellulose degradation segment in locusts; furthermore, the majority of nutritional components were absorbed in the midgut of locusts. The activity of CMCase was significantly higher than that of xylanase, and manganese peroxidase (MnPase) activity was lowest, which might be due to the basic nutrition of locusts being cellulose and hemicellulose and not lignin based on the results of FE-SEM, FTIR, XRD, and TG analysis. Overall, these results provided a valuable insight into lignocellulosic degradation mechanisms for understanding gramineous plant cell wall deconstruction and recalcitrance in locusts, which could be useful in the development of new enzymatic pretreatment processes mimicking the locust digestive system for the biochemical conversion of lignocellulosic biomass to fuels and chemicals.

Soldier Caste-Specific Protein 1 Is Involved in Soldier Differentiation in Termite Reticulitermes aculabialis.

Termite soldiers are a unique caste among social insects, and their differentiation can be induced by Juvenile hormone (JH) from workers through two molts (worker-presoldier-soldier). However, the molecular mechanism underlying the worker-to-soldier transformation in termites is poorly understood. To explore the mechanism of soldier differentiation induced by JH, the gene soldier caste-specific protein 1 (RaSsp1, NCBI accession no: MT861054.1) in R. aculabialis was cloned, and its function was studied. This gene was highly expressed in the soldier caste, and the protein RsSsp1 was similar to the JHBP (JH-binding protein) domain-containing protein by Predict Protein online. In addition, JHIII could be anchored in the hydrophobic cage of RaSsp1 as the epoxide of the JHBP-bound JH according to the protein ligand molecular docking online tool AutoDock. The functional studies indicated that knocking down of the RaSsp1 shorted the presoldier's head capsule, reduced mandible size, delayed molting time and decreased molting rate (from worker to presoldier) at the beginning of worker gut-purging. Furthermore, knocking down of the RaSsp1 had a more pronounced effect on soldier differentiation (from presoldier to soldier), and manifested in significantly shorter mandibles, rounder head capsules, and lower molting rate (from worker to presoldier) at the beginning of presoldier gut-purging. Correspondingly, the expressions of JH receptor Methoprene-tolerant (Met), the JH-inducible transcription factor Krüppel homolog1 (Kr-h1) and ecdysone signal genes Broad-complex (Br-C) were downregulated when knocking down the RaSsp1 at the above two stages. All these results that RaSsp1 may be involved in soldier differentiation from workers by binding and transporting JH.