RESUMEN
A sensitive and accurate differential-pulse polarographic method is reported for the measurement of lactate dehydrogenase (LDH) activity. The method is based on the polarographic measurement of the NAD+ formed via the enzymic reaction of LDH with pyruvate and NADH. The method was successfully applied to measure LDH activity in the serum of normal and diseased individuals.
Asunto(s)
L-Lactato Deshidrogenasa/sangre , Enfermedad , Humanos , Indicadores y Reactivos , NAD/análisis , Polarografía/métodos , Piruvatos , Valores de Referencia , Sensibilidad y EspecificidadRESUMEN
A polarographic method for the microdetermination of sulphur in organic compounds is described; it is based on oxygen-flask combustion to yield sulphate, which is then reacted with excess of barium dichromate solution in hydrochloric acid, followed by adjustment to pH 12, collection of the combined barium chromate and sulphate precipitate, and polarographic determination of the free chromate (equivalent to the sulphate originally present).