Effects of biochar on the transformation of cadmium fractions in alkaline soil.

To investigate the chemical properties in the biochar-mediated transformation of soil cadmium (Cd) fractions, the effects of biochar applied at different pyrolysis temperatures on soil Cd-fractions, pH value, and soil organic matter (SOM) were studied through an in-lab incubation experiment on contaminated soil. The results showed that the dissolved organic carbon (DOC) of CsBC300 (biochar prepared at 300 °C) was significantly higher (up to 1.31 times) than that of CsBC600 (biochar prepared at 600 °C). However, CsBC600 was more aromatic. Due to the difference in pyrolysis temperatures, the Cd deactivation mechanism of CsBC300 and CsBC600 was mainly to provide a large amount of organic matter and aromatic functional groups to the soil, respectively. The addition of these two biochar types significantly reduced the acid-extracted Cd content, by 76.56-83.52% and 70.48-76.81%, respectively. Contrastingly, it increased the residual Cd content by 2.26-2.36 and 2.08-2.29 times, respectively, which promoted the Cd transformation from the unstable to the stable state. However, CsBC300 had slightly better deactivation effect than CsBC600 on the 120th day, which was due to the decrease of soil pH and the increased SOM content. These study results can provide a theoretical reference for the remediation of Cd-contaminated alkaline soil.

Performance and microbial mechanism of eletrotrophic bio-cathode denitrification under low temperature.

Insufficient amount of carbon in wastewater and low temperatures hinder the use of biological nitrogen removal for purification of wastewaters. Nitrogen removal using cold-tolerant electrotrophic cathodic microbes is a novel and unique autotrophic denitrification technique in which electrical current, not chemicals, is used as a source of electrons. In this study, integrated MFC (RW) and open-circuit MFC (RO) were cultured and acclimatized in stages at a low temperature (10 °C) to impart cold tolerance to electrotrophic cathodic microbes, investigate the effectiveness of simultaneous nitrification and denitrification (SND) process, and address the possible mechanism of microbial action. The results showed that (i) microbial communities in the RW system were successfully enriched with the cold-tolerant electrotrophic cathodic microbes after five stages, and (ii) the degree of NH4+-N removal and SND were 75.50% and 81.91%, respectively, but the respective values in the RO system were only 40.47% and 54.01%. The desirable SND efficiency was obtained in RW at a DO of ∼0.6 mg/L, a current of ∼20 mA, and pH ∼7.0. In RW, Thauera, Pesudomonas, and Hydrogenophaga were the main electrotrophic cathodic denitrifying bacteria with cold tolerance capable of degrading ammonia, nitrate, and nitrite through autotrophic denitrification and cathodic-driven bio-electrochemical denitrification. Besides, for RW, results from high throughput sequencing analysis revealed that the abundance of genes related to energy production and conversion, amino acid transport, and metabolism, signal transduction, environmental adaptation, and enzymatic activity (AMO, HAO, NAR, NIR, NOR, and NOS) were significantly higher than the corresponding parameters of the RO system. This may explain the reason behind RW having excellent ammonia and TN removal performance at low temperatures.