Effects of salinity and concomitant species on growth of Phragmites australis populations at different levels of genetic diversity.

In plant communities, genetic diversity among dominant species can not only affect the fitness of the population, but also interactions with concomitant species. Soil salinity is a common factor that influences plant growth in estuarine wetlands. However, few studies have tested whether their high genetic diversity will be beneficial for the resistance of plant populations to salinity and the presence of concomitant plants. Four different genotypes of Phragmites australis, a dominant species of the Yellow River Delta in China, were selected to construct populations with three different genotypic levels. These populations were planted either with or without concomitant species and were subjected to control or salinity treatments. At the end of treatments, growth variables of P. australis populations were measured. In response to soil salinity, the total biomass of 1-, 2-, and 4-genotype populations decreased by 35%, 24%, and 13%, respectively, indicating higher resistance of P. australis populations with high genetic diversity. Correspondingly, 2-, and 4-genotype populations showed higher biomass allocation to roots, which can maintain adequate water uptake for plants. The biomass accumulation of 1-genotype populations with concomitant plants was significantly lower compared with populations without concomitant plants; however, no significant difference was found for 4-genotype populations between both control and salinity treatments, suggesting their higher capacities when coexisting with concomitant species. However, the genotypic level of populations did not significantly affect their biomass accumulation. High genetic diversity is greatly beneficial for the resistance of P. australis populations to salinity and coexistence with other plants. This information should be considered in the construction or restoration of this species in estuarine wetlands.

Combined effect of growth promoter roxarsone and copper on the earthworm Eisenia fetida.

Roxarsone (ROX) and copper (Cu) are growth promoters in livestock to promote growth and prevent disease. These chemicals and their metabolites are released to the soil through manure application and have a potential adverse effect on soil-dwelling organisms. The objective of this study was to investigate the combined subacute effect of ROX exposure (0, 80, 240, 720 mg kg-1) and Cu exposure (0, 80, 160 mg kg-1) in earthworms (Eisenia fetida). Growth, reproduction, spermatogenesis under light microscope, and heavy metal residue were investigated during 56-day exposure period. Results showed that Cu exposure of 80 or 160 mg kg-1 alleviated the effect of ROX on cocoon production or hatching. The cocoon number exhibited an increase (P < 0.05) at 80 mg kg-1 ROX on day 28, compared with the 0 mg kg-1 ROX, in the presence of 80 mg kg-1 Cu, whereas there was no effect (P > 0.05) in the presence of 160 mg kg-1 Cu. The hatching success at 80 or 240 mg kg-1 ROX exhibited a decrease (P < 0.05) on day 28, in the absence of Cu, whereas no effect (P > 0.05) was observed in the presence of 80 or 160 mg kg-1 Cu. The other reproductive parameters (cocoon weight, juvenile number, and biomass) demonstrated a decrease (P < 0.05) only at 720 mg kg-1 ROX in the presence or absence of Cu. However, with increasing exposure time, the above reproductive parameters were not affected (P > 0.05) in all groups on day 56. On the other hand, sperm deformity (%) increased (P < 0.05) at 240 or 720 mg kg-1 ROX on day 28, in the presence or absence of Cu; however, the microstructural alteration in seminal vesicles occurred only at 720 mg kg-1 ROX, exhibiting disordered distribution and decreased mature sperm bundles. In addition, ROX or Cu residues in earthworms demonstrated an increase with increasing ROX or Cu exposure concentration. Our present results may provide important insight on combined toxicity of chemicals in soils.

Growth responses of eight wetland species to water level fluctuation with different ranges and frequencies.

Fluctuation range and frequency are two important components of water level fluctuation, but their effects on wetland plants have not been evaluated separately. We subjected eight wetland species to a control treatment with static water level and fluctuation treatments with different ranges or frequencies to examine their effects on plant growth. Acorus calamus, Butomus umbellatus and Iris wilsonii showed high survival rates in all treatments with various fluctuation ranges and frequencies. Their survival rates were higher at the medium fluctuation frequency than at the low and high frequencies, suggesting beneficial effects of the medium frequency. In the experiment comparing the fluctuation ranges, A. calamus and I. wilsonii could maintain the capacity for asexual propagation and accumulate higher biomass compared with the control plants, while biomass of the other six species dramatically decreased. In the experiment comparing fluctuation frequency, species with relatively high survival rates (≥ 50%) maintained or increased the capacity of asexual propagation, and A. calamus and I. wilsonii allocated relatively more biomass to roots, which may enhance plant growth and survival. In contrast, these species did not show increased biomass allocation to shoots in response to both fluctuation range and frequency, presumably because shoots are prone to mechanical damage caused by streaming floodwater. Taken together, biomass accumulation in roots rather than in shoots and the ability to asexually propagate are important for the survival of these species during water fluctuation.

Effects of fermented Chinese herbal medicines on milk performance and immune function in late-lactation cows under heat stress conditions.

Heat stress (HS) causes significant economic losses and has become a continual challenge in the dairy industry worldwide. The objective of this study was to evaluate the effects of a dietary supplement on milk performance and immune function in late-lactation cows under HS conditions. The supplement was a fermented Chinese herbal medicines (CHMs) mixture consisting of 18 herbs. Forty lactating Holstein cows (560 ± 51.0 kg of initial BW, 230 ± 10.0 DIM, 16 ± 3.0 kg of milk per day) were randomly assigned into 4 treatment groups (10 cows per group). Each group was fed a dietary supplemented with 0, 25, 50, or 100 g CHMs per cow per day. Cows were housed at high ambient temperature-humidity index (average 74.5) for an experimental period of 42 d during the summer months. Milk yield, composition, immune responses involving blood lymphocyte apoptosis rate, serum biochemical parameters, and genes expression in lymphocytes were evaluated on days 14, 28, and 42, respectively. Results showed that milk yield, milk fat, and protein content were greater (all P < 0.05) for 50 or 100 g/d CHMs compared with the group without CHMs supplements throughout the experimental period. On the other hand, increasing CHMs dose demonstrated a greater lymphocyte or leukocyte count (P < 0.01). By flow cytometry analysis, early or late apoptosis rate of the lymphocytes was decreased (P < 0.05) by CHMs supplements. The immunity-related biochemistry and genes transcript responses involving cytokines (IL-1, IL-2, IL-6, and IL-12), apoptosis (Bak, Mcl-1, Bax, Bcl-2, Bcl-xl, and P53), and immunoglobulins (IgA, IgG, and IgM) were investigated. Compared with the unsupplemented group, the serum IL-2 and IL-6 levels, as well as IL-2 mRNA expression, increased (P < 0.05) for 100 g/d. However, the serum IL-1 level tended to decrease (P = 0.08) with increasing CHMs dose, and IL-1 mRNA expression was down-regulated (P = 0.02) by up to 24% for 100 g/d. Additionally, the serum Bax level decreased (P < 0.01) and Bcl-2 level increased (P = 0.01) for 100 g/d. Bax and Bak mRNA expressions were down-regulated (P < 0.05), and Bcl-2 and Bcl-xl expression were up-regulated (P < 0.05) for 50 or 100 g/d. The mRNA expressions of P53 and Mcl-1 were not affected by CHMs (P > 0.10). Besides, serum IgG levels were greater (P < 0.01) for 50 or 100 g/d, compared with unsupplemented group. In conclusion, CHMs supplements may improve milk performance and immune function in dairy cows under HS conditions.

Gene expression responses in different regions of Eisenia fetida with antiparasitic albendazole exposure.

Albendazole (ABZ) is a veterinary drug with a high efficiency against parasite. The aim of this research is to investigate and characterize the response of gene expression in different regions of earthworms Eisenia fetida in relation to ABZ exposure. In this research, the earthworms were exposed to ABZ at 0, 10, 30, 90, and 270 mg/kg concentrations for 42 days. Within the initial 14-day exposure, the expression levels of two target genes (mitochondrial large ribosomal subunit (l-rRNA) and heat shock protein (HSP90)) in different regions of earthworms were affected significantly by the different exposure concentrations of ABZ, but the growth rates were similar among the ABZ and control groups. With longer exposure time, growth rates decreased significantly after 28 days of exposure at 90 and 270 mg/kg. These results of target genes expression suggest that, at low ABZ concentrations, the middle region of earthworms is more sensitive to ABZ than the anterior and posterior regions. In the middle region, the l-rRNA expression of the ABZ-exposed groups was significantly lower than the control group, with a reduction to 23%, 25% and 31% for 10, 30 and 90 mg/kg ABZ concentrations, respectively (P<0.01). In contrast, the HSP90 expression of the ABZ groups (full range of 10 to 270 mg/kg) in the middle region increased 4.1-8.7 folds over the control group (P<0.01). In the anterior and posterior regions, the expression of the two target genes at 10mg/kg did not differ significantly among the ABZ and control groups (P>0.05), except for l-rRNA in the posterior region. The characterization and understanding of target genes expression in different regions of earthworms can provide important information on predictive early reading on the pollution of ABZ residue in soils.

Effect of high pressure treatment on microbial populations of sliced vacuum-packed cooked ham.

In this study, culture-dependent and culture-independent approaches were used to reveal the microbial diversity and dynamic changes occurring in sliced vacuum-packed cooked ham after high pressure processing (HPP, 400MPa or 600MPa for 10min at 22°C) during refrigerated storage over 90days. Direct extraction of genome DNA and total RNA from meat samples, followed by PCR-denaturing gradient gel electrophoresis (DGGE) and RT-PCR-DGGE on 16S rDNA V3 region, was performed to define the structure of the bacterial populations and active species in pressurized cooked ham. Results showed that HPP affected differently the various species detected. The predominant spoilage organisms of cooked ham, such as Lactobacillus sakei and Lactobacillus curvatus, were found to be very sensitive to pressure as they were unable to be detected in HPP samples at any time during refrigerated storage. Weissella viridescens and Leuconostoc mesenteroides survived HPP at 600MPa for 10min at 22°C and were responsible for the final spoilage. An RNA-based DGGE approach clearly has potential for the analysis of active species that have survived in pressurized cooked ham. High pressure processing at 400 or 600MPa for 10min at room temperature (22°C) has a powerful inhibitory effect on the major spoilage bacteria of sliced vacuum-packed cooked ham. High pressure treatment may lead to reduced microbial diversity and improve the products' safety.

Toxic effects of enrofloxacin on growth rate and catalase activity in Eisenia fetida.

Enrofloxacin (EFLX) has been proven effective against both gram-negative and gram-positive microorganisms and used widely in animal husbandry. It may enter the soil environment with the excreta of treated animals. The aim of this study is to determine the toxicity of EFLX on the whole earthworms as well as its different tissues. Earthworms of Eisenia fetida were exposed to EFLX at 0, 500, 1000, 3000, and 5000mg/kg concentrations; samples were taken at days 2, 7, and 14 of exposure for determination of catalase (CAT) activity and growth rate. The growth rate showed little differences in all treatment groups. CAT activity was shown to be changeable at different tissues, exposure time, and EFLX concentrations. The intestinal tissues were more sensitive to EFLX than the body wall muscle tissues and whole earthworms. The investigation of CAT activity, especially in the intestinal tissues, can provide important information in terms of toxic effects of soil contamination.

Toxic effects of albendazole on adenosine triphosphatase activity and ultrastructure in Eisenia fetida.

Veterinary drug may influence soil fauna through manure excretion and subsequent application to agricultural field. The aim of this study is to determine the toxicity of albendozale (ABZ) on the whole earthworm as well as its different regions. Earthworms of Eisenia fetida were exposed to ABZ at 0, 100, 200, 400, and 600 mg kg(-1) concentrations; samples were taken at days 2, 7, and 14 exposure for determination of two adenosine triphosphatase (Na(+)-K(+)- and Mg(2+)-ATPase) activities and survival and growth rate. In addition, the ultrastructure of intestinal epithelium of the earthworms was examined after 14-day exposure. The survival and growth rate were reduced as compared to the control at the two highest concentrations (400 and 600 mg kg(-1)) after 7- and 14-day exposure. With increasing ABZ concentration, ATPase activities were inhibited significantly in the mid-part after 7 and 14 days and the posterior after 14 days. In particular, the inhibition effect was significant even at the lower treatment levels (100 and 200 mg kg(-1)) after 14 days. Both ATPase activities, however, were increased significantly in the anterior of earthworms at the highest concentration (600 mg kg(-1)) after 14 days. Ultrastructure observation in intestine epithelium in three concentrations (control, 100, 600 mg kg(-1)) revealed that mitochondria and smooth endoplasmic reticulum were damaged with increasing ABZ concentration. Some mitochondria was exhibited the damage of inner membrane at 100 mg kg(-1) and vacuolization at 600 mg kg(-1), which is consistent with ATPase activities inhibition. The investigation of enzymatic activities in different regions of earthworms and pathological alterations in the intestinal epithelium can provide important information in terms of toxic effects of soil contamination and be used as early warning systems.