Enhancing orthodontic treatment control with fish scale-derived hydroxyapatite nanoparticles: Insights from an animal model study.

Objectives: This study investigates the impact of injected fish-scale-derived hydroxyapatite nanoparticles (FsHA-NPs) on orthodontic tooth movement (OTM) and the width of the periodontal ligament (PDL) space. Materials and Methods: Twenty-six Wistar rats underwent mesial orthodontic traction with a force of 50 g for 21 days. Following the application of the orthodontic appliance, the rats were randomly divided into two groups: a control group, which received a 0.3 µg saline injection, and the experimental FsHA group, which received 100 mg/0.3 ml of FsHA-NPs after thorough characterisation. Injections were administered immediately after appliance application and repeated at 7 and 14 days. Statistical analysis was conducted with a significance level of P ≤ 0.05. Result: The experimental group exhibited a significant reduction in OTM at 7-, 14-, and 21-day post-force application. Additionally, a reduction in PDL width was observed in the mesiocervical and disto-apical regions of the mesial and distal roots of the first molar. Conclusion: FsHA-NPs derived from biowaste fish scales exhibit promising potential as biomaterials for enhancing control over OTM. This study underscores the viability, accessibility, and safety of FsHA-NPs as a locally injectable material for orthodontic applications.

Calixarene Derivatives: A Mini-Review on their Synthesis and Demands in Nanosensors and Biomedical Fields.

Nanotechnology has been widely studied in biomedical applications in the last decade. The revolution in nanotechnology triggers the fabrication of nanomaterials with novel properties and functionalities, making the research in nanosensors and biomedical rapidly expanding. Nanosensor application has improved the sensitivity by enhancing their catalytic activity, conductivity, and biocompatibility. Calixarene is excellent as a sensing element used as a sensor due to its unique host-guest properties. Three major types of calixarene which are extensively studied are calix[4]arene, calix[6]arene, and calix[8]arene. These organic nanomaterials resemble vase-like supramolecular structures and exhibit valuable properties. Calixarene's basic molecular design is the cyclic phenol tetramer with four aryl groups, perfect for molecular recognition such as cations, transition metal ions, and heavy metals. Calixarenes may form stable complexes with biomolecules in developing biosensors for protein, enzyme, and antibody sensing. Calixarene's lower rim can be modified for optimum molecular interaction with guest molecules such as anions, cations, and neutral molecules. The lower ring has welldefined conformation properties and cavities, which allow trapping guest drugs such as imatinib, paclitaxel, and temozolomide. Calixarene also possesses good biocompatibility and innocuousness and gained attention for cancer treatment due to the response to multiple stimuli, stability, avoiding non-specific cell uptake, and reaching the target for treatment effect. This review paper focuses on the synthesis and characteristics of calixarene applied in nanosensors as an ideal complex agent in drug transportation and controlled drug released for biomedical research.