Optimizing green approach to enhanced antioxidants from Thai pigmented rice bran using deep eutectic solvent-based ultrasonic-assisted extraction.

Deep eutectic solvents (DES) have garnered significant attention as extraction media owing to their commendable attributes of being environmentally sustainable and the inherent adaptability of DES's versatile physical and chemical characteristics. The present study investigated the effects of deep eutectic solvents on the total contents of anthocyanin, phenolic, and flavonoids, as well as the antioxidant activity of Thai pigmented rice bran extract. The optimal extraction parameters for deep eutectic solvent-based ultrasonic-assisted extraction (DES-UAE) were also determined using the response surface methodology (RSM). The optimal conditions for the extraction of anthocyanins and other antioxidants from pigmented rice bran using a deep eutectic solvent were choline chloride: ethylene glycol (Ch:Eg) at a 1:2 ratio, mixed with 20 % water as a solvent. The ultrasonic-assisted extraction (UAE) at 37 kHz of frequency, 50 °C of temperature, 40 min of extraction time, and a 1:6 g/mL of solid-to-solvent ratio yielded a total anthocyanin content of 4.55 ± 0.09 mg C3G/g DW, a total phenolic content of 26.49 ± 0.62 mg GAE/g DW, a total flavonoid content of 6.57 ± 0.55 mg QE/g DW, and a percent inhibition of DPPH radical of 77.83 ± 1.51. By comparing the antioxidant content that was extracted from three cultivars of pigmented rice, it was found that Leum Pua black rice bran provided significantly higher antioxidant content compared to Hom Nin purple rice bran and Mali Dang red rice bran. This research suggests an achievable, eco-friendly, and effective method for preparing high-quality, consumer-safe Thai rice bran as a raw material for nutraceuticals.

A fractional model of magnetohydrodynamics Oldroyd-B fluid with couple stresses, heat and mass transfer: A comparison among Non-Newtonian fluid models.

The present article aims to extend some of the already existing fluid models to a large class of fluids namely, "Oldroyd-B couple stress fluid (OBCSF)". The main focus of the present work is to combine the existing fluid models in ordered to get a new class of fluid. The unsteady magnetohydrodynamics (MHD) Oldroyd-B fluid (OBF) with couple stresses, porosity, heat and mass transfer is considered in the present analysis. The Oldroyd-B couple stress fluid is assumed to flow in channel. The classical model is fractionalized by considering Atangana-Baleanu (AB) operator in ordered to highlight the memory analysis. To develop closed form solutions the combined (Laplace + Fourier) integrals have been used. The results obtained are portrayed through graphs for all pertinent flow parameters which involved in the present dynamic model. Moreover, the impact of AB time fractional parameter is investigated graphically on flow, temperature and concentration distributions exploiting MATHCAD software. Secondly, for better understanding the present solutions of Oldroyd-B couple stress fluid (OBCSF) are reduced to Odroyd-B fluid (OBF) without couple stresses, Maxwell solutions, Couple stress solutions and Newtonian viscous fluid solutions and the results have been compared for classical and fractional order derivatives. In addition to this a limiting case is carried out by our solutions to already published work which verify our solutions. In addition to this during the analysis we noticed that the flow heat and concentrated get lowered for the escalating numerical values of AB fractional derivatives. Similarly, it is also noticed that the velocity in channel accelerated with the increment of numeric values of pressure, porosity, thermal buoyancy and relaxation time parameter. In the same manner temperature and concertation profiles gets low with the higher values of Prandtl number, Reynold number and fractional operator. Finally, skin friction for momentum equation, Nusselt number for temperature and Sherwood number for concentration have been calculated and given in tabular forms.

Exact solutions via Prabhakar fractional approach to investigate heat transfer and flow features of hybrid nanofluid subject to shape and slip effects.

The core devotion of this study is to develop a generalized model by means of a recently proposed fractional technique in order to anticipate the enhancement in the thermal efficiency of engine oil because of the dispersion of graphene and magnesia nanoparticles. In addition to investigating the synergistic attributes of the foregoing particles, this work evaluates shape impacts for column, brick, tetrahedron, blade, and lamina-like shapes. In the primary model, the flow equation is coupled with concentration and energy functions. This classical system is transmuted into a fractional environment by generalizing mathematical expressions of thermal and diffusion fluxes by virtue of the Prabhakar fractional operator. In this study, ramped flow and temperature slip conditions are simultaneously applied for the first time to examine the behavior of a hybrid nanofluid. The mathematical analysis of this problem involves the incorporation of dimension-independent parameters into the model and the execution of the Laplace transform for the consequent equations. By doing so, exact solutions are derived in the form of Mittag-Leffler functions. Multiple illustrations are developed by dint of exact solutions to chew over all aspects of temperature variations and flow dynamics. For the preparation of these illustrations, the details of parametric ranges are as follows: [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text], and [Formula: see text]. The contribution of differently shaped nanoparticles, volume proportions, and fractional parameters in boosting the heat-transferring attributes of engine oil is also anticipated. In this regard, results for Nusselt number are provided in tabular form. Additionally, a brief analysis of shear stress is carried out for fractional parameters and various combinations of magnesia, graphene, and engine oil. This investigation anticipates that engine oil's hybridization with magnesia and graphene would result in a 33% increase in its thermal performance, which evidently improves its industrial significance. The enhancement in Schmidt number yields an improvement in the mass transfer rate. An increment in collective volume fraction leads to raising the profile of the thermal field. However, the velocity indicates a decreasing behavior. Nusselt number reaches its highest value ([Formula: see text]) for the lamina shape of considered particles. When the intensity of the buoyancy force augments, it causes the velocity to increase.

Significance of Hall current and viscous dissipation in the bioconvection flow of couple-stress nanofluid with generalized Fourier and Fick laws.

In the pump of different machines, the vacuum pump oil (VPO) is used as a lubricant. The heat rate transport mechanism is a significant requirement for all industries and engineering. The applications of VPO in discrete fields of industries and engineering fields are uranium enrichment, electron microscopy, radio pharmacy, ophthalmic coating, radiosurgery, production of most types of electric lamps, mass spectrometers, freeze-drying, and, etc. Therefore, in the present study, the nanoparticles are mixed up into the VPO base liquid for the augmentation of energy transportation. Further, the MHD flow of a couple stress nanoliquid with the applications of Hall current toward the rotating disk is discussed. The Darcy-Forchheimer along with porous medium is examined. The prevalence of viscous dissipation, thermal radiation, and Joule heating impacts are also considered. With the aid of Cattaneo-Christov heat-mass flux theory, the mechanism for energy and mass transport is deliberated. The idea of the motile gyrotactic microorganisms is incorporated. The existing problem is expressed as higher-order PDEs, which are then transformed into higher-order ODEs by employing the appropriate similarity transformations. For the analytical simulation of the modeled system of equations, the HAM scheme is utilized. The behavior of the flow profiles of the nanoliquid against various flow parameters has discoursed through the graphs. The outcomes from this analysis determined that the increment in a couple-stress liquid parameter reduced the fluid velocity. It is obtained that, the expansion in thermal and solutal relaxation time parameters decayed the nanofluid temperature and concentration. Further, it is examined that a higher magnetic field amplified the skin friction coefficients of the nanoliquid. Heat transport is increased through the rising of the radiation parameter.

Analysis of second grade hybrid nanofluid flow over a stretching flat plate in the presence of activation energy.

The research of fluid containing nanoparticles for the heat transport characteristics is very famous because of its variety of real-life applications in various thermal systems. Although the thermal efficiency of the nanofluid was effective but still the nano scientists were trying to introduce some new advance class of fluid. Therefore, an advance class of fluid is developed by the dispersion of two different nano sized particles in the conventional base fluid known as "Hybrid nanofluid" which is more effective compared to simple nanofluids in many engineering and industrial applications. Therefore, motivated from the hybrid type of nanofluids in the current research we have taken two-dimensional laminar and steady flow of second grade fluid passing through porous plate. The engine oil base fluid is widely used fluid in the engineering and industrial problems. Keeping these applications in mind the engine oil is considered and two different nanoparticles Copper and aluminum oxide are added in ordered to get the required thermal characteristics. In addition to this the thermal radiation, chemical reaction, activation energy, Brownian motion and thermophoresis are also addressed during the current research. The present proposed higher-order PDE's is transformed to the non-linear system of ODE's. For the solution of the proposed high non-linear model HAM method is employed. As the hybrid nanofluid are highlighted on the second-grade fluid flow over a horizontal porous flat plate. During the present analysis and experimental study, it has been proved that the performance of hybrid nanofluid is efficient in many situations compared to nanofluid and regular fluid. For physical interpretation all the flow parameters are discussed through graphs. The impact of volume fraction is also addressed through graphs. Moreover, the comparative analysis between hybrid and nanofluid is carried out and found that hybrid nanofluid performed well as compared to nanofluid and regular fluid. The engineering quantities obtained from the present research have been presented in tables.

Electromagnetic Trihybrid Ellis Nanofluid Flow Influenced with a Magnetic Dipole and Chemical Reaction Across a Vertical Surface.

The purpose of this study is to evaluate the augmentation of thermal energy transfer in trihybrid Ellis nanofluid flow in the occurrence of magnetic dipole passes over a vertical surface. The ternary hybrid nanofluid is prepared by the dispersion of ternary nanoparticles (Al2O3, SiO2, and TiO2) in the Carreau Yasuda fluid. The velocity and heat transportation has been examined in the existence of the Darcy Forchhemier influence and heat source/sink. The phenomena of fluid flow have been mathematically designed for energy and fluid velocity in the form of a nonlinear partial differential equation (PDE)-based system. The system of PDEs is further refined to the set of ordinary differential equations via suitable similarity substitutions. The acquired dimensionless equations are numerically solved with the help of the HAM. It has been noticed that the energy contour is enhanced versus the variation of viscous dissipation and heat generation. A significant contribution of a magnetic dipole is observed to elevate the production of the thermal energy field, and an opposite trend is noticed versus the flow profile. The accumulation of Al2O3, SiO2, and TiO2 nanomaterials in the base fluid "engine oil" improves the velocity and energy profiles.

Polyester-releasing sesamin by electrospinning technique for the application of bone tissue engineering.

Sesamin, a significant lignin compound isolated from sesame (Sesamum indicum Linn), is well known for its antioxidant, anti-inflammatory, and tissue growth promotion properties. Bioabsorbable poly(ε-caprolactone) (PCL) is also a well-known polymer applied to various fields of medicine as biomaterials. The main objective of this research was to produce a prototype material from PCL and sesamin by electrospinning technique for bone tissue engineering applications. Dichloromethane and dimethylformamide (7:3) mixture was used as the solvent system for fabrication of PCL nanofiber with different loads of sesamin concentrations (1-6 wt%). The crystallinity levels decreasing and the entrapment efficiency increasing (86.87%-93.97%) were observed while sesamin concentrations were increased. The infrared spectra of electrospun mats confirmed that sesamin corporated into fibrous networks. The sesamin-loaded PCL nanofibrous membranes showed a significant release of sesamin in the range of 1.28-8.16 µg/mL within 10 weeks. The release data were fitted to zero order, first order, Higuchi and Korsmeyer-Peppas models to evaluate sesamin-releasing mechanisms and kinetics. The releasing kinetics of sesamin followed the Fickian diffusion mechanism of Korsmeyer-Peppas (R2 = 0.99). In vitro experiments with an osteosarcoma cell line (MG-63) revealed cell attachment, biocompatibility, and promotion of bone marker expression, the alkaline phosphatase (ALP) activity were studied. The electrospun PCL nanofiber loaded with sesamin had the potential as a scaffold for sesamin delivery to bone cells and applications in biomedicine.

Microwave-Assisted Extraction of Anticancer Flavonoid, 2',4'-Dihydroxy-6'-methoxy-3',5'-dimethyl Chalcone (DMC), Rich Extract from Syzygium nervosum Fruits.

2',4'-Dihydroxy-6'-methoxy-3',5'-dimethyl chalcone (DMC) is a biological flavonoid that is present in the fruits of Syzygium nervosum (Ma-Kiang in Thai). Microwave-assisted extraction (MAE), which utilizes microwave radiation to heat the extraction solvent quickly and effectively, was used to recover DMC-rich extract from Syzygium nervosum fruit. To determine the DMC content, a highly accurate and precise HPLC technique was developed. The influences of MAE conditions, including the solid-liquid ratio, microwave power, and microwave duration on the content of DMC, were sequentially employed by a single factor investigation and response surface methodology (RSM) exploratory design. The predicted quadratic models were fitted due to their highly significant (p < 0.0001) and excellent determination coefficient (R2 = 0.9944). The optimal conditions for producing DMC-rich extract were a ratio of sample to solvent of 1:35 g/mL, a microwave power of 350 W, and a microwave time of 38 min. Under the optimal MAE setting, the DMC content reached 1409 ± 24 µg/g dry sample, which was greater than that of the conventional heat reflux extraction (HRE) (1337 ± 37 µg/g dry sample) and maceration (1225 ± 81 µg/g dry sample). The DMC-rich extract obtained from MAE showed stronger anticancer activities against A549 (human lung cancer cells) and HepG2 (human liver cancer cells) than the individual DMC substance, which makes MAE an effective method for extracting essential phytochemicals from plants in the nature.

Variation in the Antibacterial and Antioxidant Activities of Essential Oils of Five New Eucalyptus urophylla S.T. Blake Clones in Thailand.

Eucalyptus oils are widely used for a variety of purposes. This study investigates the terpenoid compositions and antibacterial and antioxidant activities of eucalypt leaf oils extracted from four E. urophylla clones and one E. urophylla × E. camaldulensis hybrid clone grown in Thailand. According to GC/MS analysis, the E. urophylla oils were mainly composed of 1,8-cineole, α-terpinyl acetate, ß-caryophyllene, and spathulenol, while 1,8-cineole, α-terpinyl acetate, p-cymene, and γ-terpinene were mostly identified in the hybrid oil. All eucalypt oils exhibited a significant bacteriostatic effect against Gram-positive bacteria, Streptococcus pyogenes, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus. Only the hybrid oil had an effect on all Gram-negative bacteria tested, including Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, and Enterobacter aerogenes. These oils have antibacterial properties that vary according to their terpenoid content. Only the hybrid oil had a potent antioxidant effect, with an IC50 value of 4.21 ± 0.35 mg/mL for free radical (DPPH) scavenging. This oil's antioxidant effect may be a result of the phenolic terpenoids, thymol and carvacrol. As a result, these oils may be a novel source of antibacterial and antioxidant agents. Additionally, the antibacterial and antioxidant capabilities of the E. urophylla × E. camaldulensis hybrid essential oil are reported for the first time.

Microwave-Assisted Improved Extraction and Purification of Anticancer Nimbolide from Azadirachta indica (Neem) Leaves.

Nimbolide, a limonoid present in leaves of the neem tree (Azadirachta indica), is an anticancer compound against a panel of human cancer cell lines. The rapid process of extraction and purification of the nimbolide from the leaves of neem tree through microwave-assisted extraction (MAE) coupled with a chromatographic technique was accomplished. The crude with a maximum content of nimbolide could be recovered from neem leaves through MAE. By using three-factors, three-level Box-Behnken design of response surface methodology (RSM), the optimal conditions for nimbolide extraction (R2 = 0.9019) were solid/liquid ratio 1:16 g/mL, microwave power 280 W, and extraction time 22 min. The enriched extract was further purified by a preparative thin-layer chromatography (PTLC), where nimbolide was obtained as 0.0336 g (0.67% yield, purity over 98%) with ethyl acetate/hexane = 4:6 in 3.0 h. Structural elucidation was performed through spectroscopic techniques, including FT-IR, 1H, and 13C-NMR. This method was simple and had a good potential for the purification of bioactive compounds from a natural product.

Simultaneous quantification of vitamin E, γ-oryzanols and xanthophylls from rice bran essences extracted by supercritical CO2.

Since the nutrition value of rice is diminished during rice processing, technology that can preserve and sustain functional compounds is necessary. In this study, supercritical carbon dioxide (SC-CO2) extraction was optimized for operational conditions (time, temperature, pressure and modifier) to extract vitamin E, γ-oryzanols and xanthophylls from rice bran. The simultaneous quantification of the compounds was developed using high-performance liquid chromatography with diode array and fluorescence detectors. Central composite design and respond surface methodology were applied to achieve optimum extraction conditions. The optimized conditions were 60min, 43°C, 5420psi with 10% ethanol as a modifier. Pigmented rice bran extracts contained greater amounts of functional phytochemicals than non-pigmented rice bran extracts (0.68, 1410, and non-detectable µg/g compared with 16.65, 2480, and 0.10µg/g of vitamin E, γ-oryzanols and xanthophylls in pigmented and non-pigmented ones, respectively). SC-CO2 extraction with modifier would be promising for preparation of phytochemical essences for therapeutic purpose.

Anti-Tumor and Immune Enhancing Activities of Rice Bran Gramisterol on Acute Myelogenous Leukemia.

BACKGROUND: Acute myelogenous leukemia (AML) is a cancer of the blood that most commonly affects human adults. The specific cause of AML is unclear, but it induces abnormality of white blood cells that grow rapidly and accumulate in bone marrow interfering with the production and functions of the normal blood cells. AML patients face poor prognosis and low quality of life during chemotherapy or transplantation of hematopoietic stem cells due to the progressive impairment of their immune system. The goal of this study is to find natural products that have the potential to delay growth or eliminate the abnormal leukemic cells but cause less harmful effect to the body's immune system. METHODS AND FINDINGS: The unsaponified fraction of Riceberry rice bran (RBDS) and the main pure compound, gramisterol, were studied for cytotoxicity and biological activities in WEHI-3 cells and in the leukemic mouse model induced by transplantation of WEHI-3 cells intraperitoneally. In the in vitro assay, RBDS and gramisterol exerted sub-G1 phase cell cycle arrest with a potent induction of apoptosis. Both of them effectively decreased cell cycle controlling proteins (cyclin D1 and cyclin E), suppressed cellular DNA synthesis and mitotic division, and reduced anti-apoptosis Bcl-2 protein, but increased apoptotic proteins (p53 and Bax) and activated caspase-3 enzyme in the intrinsic cell death stimulation pathway. In leukemic mice, daily feeding of RBDS significantly increased the amount of immune function-related cells including CD3+, CD19+, and CD11b+, and elevated the serum levels of IFN-γ, TNF-α, IL-2, and IL-12ß cytokines, but suppressed IL-10 level. At the tumor sites, CD11b+ cells were polarized and became active phagocytotic cells. Treatment of mice normal immune cells with gramisterol alone or a combination of gramisterol with cytokines released from RBDS-treated leukemic mice splenocytes culture synergistically increased pSTAT1 transcriptional factor that up-regulated the genes controlling cell survival and function. Phosphorylation of STAT1 was absent in WEHI-3. Instead, similar treatments significantly decreased pSTAT3 signaling that regulates transcription of genes controlling tumor growth and proliferation. CONCLUSIONS: Rice bran gramisterol possesses a promising anti-cancer effect against a tumor of white blood cells and induces the production of anti-cancer immune-related cytokines. Gramisterol induces cell cycle arrest and apoptosis via suppression of pSTAT3 signaling control of tumor cells' growth and progression. Gramisterol increased IFN-γ production and prevented the dysfunctional immune system of leukemic mice by enhancing pSTAT1 transcription signal controlling proliferation and functions of hematopoietic cells in the spleen. Together with IFN-γ, gramisterol efficiently facilitates leukemic mice immune system modulation leading to improvement of the AML condition. Administration of RBDS containing gramisterol potentiates immune recovery of leukemic mice and extends their survival. This finding encourages the medicinal application of rice bran gramisterol as a palliative treatment or an alternative agent for future drug development against AML.

Structures of phytosterols and triterpenoids with potential anti-cancer activity in bran of black non-glutinous rice.

Structures of some bioactive phytochemicals in bran extract of the black rice cv. Riceberry that had demonstrated anti-cancer activity in leukemic cell line were investigated. After saponification with potassium hydroxide, separation of the unsaponified fraction by reversed-phase high performance liquid chromatography (HPLC) resulted in four sub-fractions that had a certain degree of anti-proliferation against a mouse leukemic cell line (WEHI-3 cell), this being IC50 at 24 h ranging between 2.80-467.11 µg/mL. Further purification of the bioactive substances contained in these four sub-fractions was performed by normal-phase HPLC. Structural characterization by gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance spectroscopy (NMR) resulted in, overall, the structures of seven phytosterols and four triterpenoids. Four phytosterols, 24-methylene-ergosta-5-en-3ß-ol, 24-methylene-ergosta-7-en-3ß-ol, fucosterol, and gramisterol, along with three triterpenoids, cycloeucalenol, lupenone, and lupeol, were found in the two sub-fractions that showed strong anti-leukemic cell proliferation (IC50 = 2.80 and 32.89 µg/mL). The other sterols and triterpenoids were campesterol, stigmasterol, ß-sitosterol and 24-methylenecycloartanol. Together with the data from in vitro biological analysis, we suggest that gramisterol is a significant anti-cancer lead compound in Riceberry bran extract.