Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites.

Kerosene is widely used in Ethiopia as a household fuel (for lighting and heating), as a solvent in paint and grease, and as a lubricant in glass cutting. It causes environmental pollution and escorts to loss of ecological functioning and health problems. Therefore, this research was designed to isolate, identify, and characterize indigenous kerosene-degrading bacteria that are effective in cleaning ecological units that have been contaminated by kerosene. Soil samples were collected from hydrocarbon-contaminated sites (flower farms, garages, and old-aged asphalt roads) and spread-plated on mineral salt medium (Bushnell Hass Mineral Salts Agar Medium: BHMS), which consists of kerosene as the only carbon source. Seven kerosene-degrading bacterial species were isolated, 2 from flower farms, 3 from garage areas, and 2 from asphalt areas. Three genera from hydrocarbon-contaminated sites were identified, including Pseudomonas, Bacillus, and Acinetobacter using biochemical characterization and the Biolog database. Growth studies in the presence of various concentrations of kerosene (1% and 3% v/v) showed that the bacterial isolates could metabolize kerosene as energy and biomass. Thereby, a gravimetric study was performed on bacterial strains that proliferated well on a BHMS medium with kerosene. Remarkably, bacterial isolates were able to degrade 5% kerosene from 57.2% to 91% in 15 days. Moreover, 2 of the most potent isolates, AUG2 and AUG1, resulted in 85% and 91% kerosene degradation, respectively, when allowed to grow on a medium containing kerosene. In addition, 16S rRNA gene analysis indicated that strain AAUG1 belonged to Bacillus tequilensis, whereas isolate AAUG showed the highest similarity to Bacillus subtilis. Therefore, these indigenous bacterial isolates have the potential to be applied for kerosene removal from hydrocarbon-contaminated sites and the development of remediation approaches.

Aptamer-Based Tumor-Targeted Diagnosis and Drug Delivery.

Early cancer identification is crucial for providing patients with safe and timely therapy. Highly dependable and adaptive technologies will be required to detect the presence of biological markers for cancer at very low levels in the early stages of tumor formation. These techniques have been shown to be beneficial in encouraging patients to develop early intervention plans, which could lead to an increase in the overall survival rate of cancer patients. Targeted drug delivery (TDD) using aptamer is promising due to its favorable properties. Aptamer is suitable for superior TDD system candidates due to its desirable properties including a high binding affinity and specificity, a low immunogenicity, and a chemical composition that can be simply changed.Due to these properties, aptamer-based TDD application has limited drug side effect along with organ damages. The development of aptasensor has been promising in TDD for cancer cell treatment. There are biomarkers and expressed molecules during cancer cell development; however, only few are addressed in aptamer detection study of those molecules. Its great potential of attachment of binding to specific target molecule made aptamer a reliable recognition element. Because of their unique physical, chemical, and biological features, aptamers have a lot of potential in cancer precision medicine.In this review, we summarized aptamer technology and its application in cancer. This includes advantages properties of aptamer technology over other molecules were thoroughly discussed. In addition, we have also elaborated the application of aptamer as a direct therapeutic function and as a targeted drug delivery molecule (aptasensor) in cancer cells with several examples in preclinical and clinical trials.

Biomolecules from Serratia sp. CS1 indigenous to Ethiopian natural alkaline lakes: biosurfactant characteristics and assessment of compatibility in a laundry detergent.

In this study, the biosurfactants (Bio-SFs) producing bacteria are screened from the selected alkaline lake of Ethiopia, and the potential bacterial strain and their produced Bio-SFs are further characterized. In an initial screening, 25 bacterial isolates were isolated, and among those, the bacterial isolate assigned as CS1 was identified as the most potent producer of Bio-SFs using a subsequent characterization process. The CS1 strain was identified as Serratia sp. via biochemical and molecular methods. An emulsion index (E24) of 69.06 ± 0.11% was obtained for CS1 after 5 days of incubation time at 30 °C. The CS1-extracted Bio-SFs were characterized by Fourier transform infrared (FTIR), and it indicated that the type of biosurfactant produced was a glycolipid. The stability of the crude Bio-SFs was characterized, and the optimal conditions were found to be 80 °C, pH 8, and 3% NaCl, respectively. The extracted Bio-SFs were compatible with tested commercial detergents, and its efficiency increased from 12.2 ± 0.1% to 67.1 ± 0.17% and 70.43 ± 0.11% when combined with commercially available detergent brands in Ethiopia such as Taza and Largo, respectively. This study suggests that the isolated S. marcescens CS1 strain has the potential to produce Bio-SFs that are viable competence to replace the use of synthetic chemicals in the production of commercial detergents.

Isolation and characterization of pectinase-producing bacteria (Serratia marcescens) from avocado peel waste for juice clarification.

BACKGROUND: Bacterial pectinase is an enzyme that could be employed in numerous sectors to break down pectin polysaccharide compounds. The goal of this study is to find pectinase-producing bacteria in avocado peel waste and see if the pectinase enzyme produced can be used to make fruit juice clarification. RESULTS: The researchers isolated four different bacterial strains from avocado peel waste samples. The potential two bacterial isolates that were identified as being Serratia marcescens and Lysinibacillus macrolides. Finally, the analysis of pectinase production and its application in fruit juice clarification were performed using one of the bacterial strains of Serratia marcescens. The clear apple, lemon, and mango juices were further processed to assess each juice's properties. The highest antioxidant activity was recorded in lemon juice samples. The lemon juice showed the highest total titratable acidity and total phenol content. Apple juices contained the highest total soluble solids, reducing sugar content, and viscosity and the mango juices have the maximum pH value recorded. CONCLUSIONS: The pectinase isolated from the bacterium Serratia marcescens could clear fruit juices. This pectinase needs to be studied more to make sure it works better in the fruit industry and other businesses.

Mycelium-Based Composite: The Future Sustainable Biomaterial.

Because of the alarming rate of human population growth, technological improvement should be needed to save the environment from pollution. The practice of business as usual on material production is not creating a circular economy. The circular economy refers to an economic model whose objective is to produce goods and services sustainably, by limiting the consumption and waste of resources (raw materials, water, and energy). Fungal-based composites are the recently implemented technology that fulfills the concept of the circular economy. It is made with the complex of fungi mycelium and organic substrates by using fungal mycelium as natural adhesive materials. The quality of the composite depends on both types of fungi and substrate. To ensure the physicochemical property of the fabricated composite, mycelium morphology, bimolecular content, density, compressive strength, thermal stability, and hydrophobicity were determined. This composite is proven to be used for different applications such as packaging, architectural designs, walls, and insulation. It also has unique features in terms of low cost, low emission, and recyclable.

Isolation and Characterization of Diesel-Degrading Bacteria from Hydrocarbon-Contaminated Sites, Flower Farms, and Soda Lakes.

Hydrocarbon-derived pollutants are becoming one of the most concerning ecological issues. Thus, there is a need to investigate and develop innovative, low-cost, eco-friendly, and fast techniques to reduce and/or eliminate pollutants using biological agents. The study was conducted to isolate, characterize, and identify potential diesel-degrading bacteria. Samples were collected from flower farms, lakeshores, old aged garages, asphalt, and bitumen soils and spread on selective medium (Bushnell Haas mineral salt agar) containing diesel as the growth substrate. The isolates were characterized based on their growth patterns using optical density measurement, biochemical tests, and gravimetric analysis and identified using the Biolog database and 16S rRNA gene sequencing techniques. Subsequently, six diesel degraders were identified and belong to Pseudomonas, Providencia, Roseomonas, Stenotrophomonas, Achromobacter, and Bacillus. Among these, based on gravimetric analysis, the three potent isolates AAUW23, AAUG11, and AAUG36 achieved 84%, 83.4%, and 83% diesel degradation efficiency, respectively, in 15 days. Consequently, the partial 16S rRNA gene sequences revealed that the two most potent bacterial strains (AAUW23 and AAUG11) were Pseudomonas aeruginosa, while AAUG36 was Bacillus subtilis. This study demonstrated that bacterial species isolated from hydrocarbon-contaminated and/or uncontaminated environments could be optimized to be used as potential bioremediation agents for diesel removal.

Isolation and Characterization of Urease-Producing Soil Bacteria.

Urease is an enzyme produced by ureolytic microorganisms which hydrolyzes urea into ammonia and carbon dioxide. Microbial urease has wide applications in biotechnology, agriculture, medicine, construction, and geotechnical engineering. Urease-producing microbes can be isolated from different ecosystems such as soil, oceans, and various geological formations. The aim of this study was to isolate and characterize rapid urease-producing bacteria from Ethiopian soils. Using qualitative urease activity assay, twenty urease-producing bacterial isolates were screened and selected. Among these, three expressed urease at high rates as determined by a conductivity assay. The isolates were further characterized with respect to their biochemical, morphological, molecular, and exoenzyme profile characteristics. The active urease-producing bacterial isolates were found to be nonhalophilic to slightly halophilic neutrophiles and aerobic mesophiles with a range of tolerance towards pH (4.0-10.0), NaCl (0.25-5%), and temperature (20-40°C). According to the API ZYM assays, all three isolates were positive for alkaline phosphatase, leucine aryl amidase, acid phosphatase, and naphthol_AS_BI_phosphohydrolase. The closest described relatives of the selected three isolates (Isolate_3, Isolate_7, and Isolate_11) were Bacillus paramycoides, Citrobacter sedlakii, and Enterobacter bugandensis with 16S rRNA gene sequence identity of 99.0, 99.2, and 98.9%, respectively. From the study, it was concluded that the three strains appear to have a relatively higher potential for urease production and be able to grow under a wider range of growth conditions.

Application of Microbial Bioenzymes in Soil Stabilization.

Soil stabilization is a mechanical or chemical alteration of one or more soil properties to create an improved soil material possessing the desired engineering properties. The aim of this article was to review bioenzyme-based soil stabilization techniques with an emphasis on bioenzymes production, mechanism of soil stabilization and future challenges, and opportunities of the sector. Soils are stabilized to increase strength and durability or to prevent erosion and dust generation. Cost-effective soil stabilization technology has been a fundamental part of any construction and is very important for economic growth in any country. In some cases, construction has been challenged due to the high cost of soil stabilization processes. Besides, methods of stabilizations using common stabilizing agents are getting costly. Currently, there is a growing interest to identify new and green technology to improve construction techniques and to expand the road network. Therefore, the search for new materials and improved techniques to process the local materials has received an increased focus. For developing countries, bioenzymes are now creating an opportunity to improve soil stability with tremendous effectiveness in the overall process of soil stabilization. In the world, bioenzymes have been used in different projects for several years and are generally proprietary products, often of patented formulation that needs intensive field tests. Currently, the use and production of bioenzymes is becoming the most promising key for the advancement of a country by saving time, energy, and finance. It also reduces environmental pollution due to carbon emission by the conventional stabilizers. Thus, a better understanding of this emerging technology is of utmost importance to exploit any improvement it can offer to soil stability. With little research and practice, it is possible to produce soil stabilizing bioenzymes using local raw materials. Due to this, production of low cost, easily and widely applicable, and environmentally friendly enzymatic formulations from locally available raw materials should be the interest of research and academic institutes of any country.

Dendritic Cells Activate and Mature after Infection with Mycobacterium tuberculosis.

BACKGROUND: Dendritic cells (DCs) can take up an array of different antigens, including microorganisms which they can process and present more effectively than any other antigen presenting cell. However, whether the interaction between the human DC and Mycobacterium tuberculosis represents a defense mechanism by the invaded host, or helping the invader to evade the defense mechanism of the host is still not clearly understood. FINDINGS: To analyze the interactions between M. tuberculosis and immune cells, human peripheral blood monocyte-derived immature DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry was used to analyse cell surface expression markers. The ability of the M. tuberculosis infected DC to induce T cell proliferation using 5 and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was also investigated. DCs were found to internalize the mycobacteria and show dose dependent infection and necrosis with different multiplicity of infection. Flow cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation following infection with M. tuberculosis in comparison to immature DC with no stimulation. Lipopolysaccharide (LPS) from Salmonella abortus equi, a known DC maturation agent, was used as a positive control and showed a comparable up regulation of cell surface markers as observed with M. tuberculosis infected DC. It was revealed that the M. tuberculosis infected DC induced T cell proliferation. CONCLUSION: These data clearly demonstrate that M. tuberculosis induces activation and maturation of human monocyte-derived immature DC as well as induces T cell proliferation in vitro.