Evaluation of toxicity and mutagenicity of a synthetic effluent containing azo dye after Advanced Oxidation Process treatment.

Approximately 20% industrial water pollution comes from textile dyeing process, with Azo dyes being a major problem in this scenario and requiring new forms of efficient treatment. Effluent treatments using the Advanced Oxidation Processes (AOP) are justified by the potential of application in the dyed effluent treatments once they can change the Azo dye chemical structure. Thus, this study aimed to evaluate the toxicity and mutagenic capacity of a synthetic effluent containing Amido Black 10B (AB10B) azo dye before treatment with AOP, named Gross Synthetic Effluent (GSE), and after the AOP, named Treated Synthetic Effluent (TSE). Daphnia magna and Allium cepa tests were used to evaluate acute toxicity effects and chromosomal mutagenesis, respectively. The Salmonella/microsome assay was performed to evaluate gene mutations. In silico assays were also performed aiming to identify the mutagenic and carcinogenic potential of the degradation byproducts of AB10B. There was 100% immobility to D. magna after 24 h and 48 h of treatments with TSE, showing EC50 values around 5%, whereas GSE did not show acute toxicity. However, GSE induced chromosomal mutations in A. cepa test. Both GSE and TSE were not able to induce gene mutations in S. typhimurium strains. These effects can be associated with two byproducts generated with the cleavage of the azo bonds of AB10B, 4-nitroaniline and -2-7-triamino-8-hydroxy-3-6-naphthalinedisulfate (TAHNDS). In conclusion, AOP is an efficient method to reduce the mutagenicity of synthetic effluent containing AB10B and additional methods should be applied aiming to reduce the toxicity.

Quantitative evaluation of total volatile organic compounds in urban and rural schools of southern Brazil.

Volatile organic compounds, VOCs, are air pollutants widely produced by biogenic and anthropogenic sources. This work quantitatively studied the presence of these gases in the internal and external environments of schools, comparing one in an urban area (La Salle School, Canoas, RS) and another in a rural area (Santa Cassia Farm School, Nova Santa Rita, RS). The aim of this study was to compare if this environmental differences (location) influence their gases concentration. Monitoring campaigns were conducted for 6 months, occurring every 2 weeks in both schools during class hours, 1 day indoors and 1 day outdoors. The results showed higher concentrations of total volatile organic compounds in the urban school external environment compared with the same rural school environment and, in the comparison between environments, the internal environments of the two schools obtained higher VOC concentrations than the external ones, except in November and December at the urban school.

Evaluation of the efficiency of coagulation/flocculation and Fenton process in reduction of colour, turbidity and COD of a textile effluent.

The present study investigated the efficiency of physicochemical processes of coagulation and flocculation and Fenton advanced oxidative process in reducing the parameters of colour, turbidity and Chemical Oxygen Demand (COD) of a real effluent from a textile industry. During the physicochemical process, the efficiencies of different coagulants (aluminium polychloride (Polifloc 18), ferric chloride (Acquafloc FC40), aluminium sulphate combined with organic coagulant (AST) and aluminium sulphate) and nonionic (FX NS2), cationic (FX CS6 and FX CS7) and anionic (FX AS6 and AN905) flocculants were tested. After the tests, 72.60% of COD, 36.25% of colour and 98.59% of turbidity were removed, using aluminium polychloride coagulant and AN 905 flocculant. It was also evaluated the effect of Fenton advanced oxidative process application in removal of colour, COD and turbidity of the effluent previously treated through the physicochemical process. Removals of these parameters were analysed in two different pH ranges (pH 6.0 and 7.0). In both pH 6.0 and pH 7.0, reductions were observed in all analysed parameters, obtaining 170.78 mg O2/L of COD, 22.19 mg/L of colour and 0.80 NTU of turbidity (at pH 6.0) and 151.80 mg O2/L of COD, 26.73 mg/L of colour, 0.94 NTU of turbidity (at pH 7.0), which demonstrates the efficiency of this process in the reduction of parameters analysed.

Treatment of re-refining effluent from lubricating oils by combining electrocoagulation and coagulation-flocculation processes.

A combination of electrocoagulation and coagulation-flocculation processes was used for re-refining effluent from lubricating oils. The efficiency of the process was evaluated based on the chemical oxygen demand (COD), color, and turbidity of the refined effluent. Electrocoagulation (EC) and coagulation-flocculation parameters, such as the initial pH (3.00, 4.41, and 9.00), and current density (4, 9, and 16 A/m2), and the use of aluminum polychloride coagulant and superfloc A300 flocculant were studied. EC performed at pH 9, with a current density of 16 A/m2 and 7 V, resulted in removal efficiencies of 85.14%, 99.81%, and 99.85%, for COD, color, and turbidity, respectively. The removal efficiencies increased to 96%, 99.87%, and 99.94% for COD, color, and turbidity, respectively, by the further coagulation-flocculation treatment in the presence of 13.8 mg/L aluminum polychloride coagulant and 80 mg/L Superfloc A300 flocculant.

Amido Black 10B a widely used azo dye causes DNA damage in pro- and eukaryotic indicator cells.

Acid Black 10B (AB10B) is widely used for the production of textiles, leather and prints. It is a representative of azo dyes and it is well documented that some of these compounds are mutagenic per se, and that cleavage products (in particular aromatic amines) may cause damage of the genetic material and cancer. Since no toxicological data on AB10B have been published, we evaluated its mutagenic activity in Salmonella/microsome assays and studied its acute toxic and genotoxic properties in a human derived liver cell line (HepG2) which retained the activities of drug metabolizing enzymes. The compound did not cause cytotoxicity (MTT assay), but clear genotoxic effects were detected in pro- and eukaryotic indicator cells. Dose dependent induction of his+ revertants was seen in strain TA98 which detects frameshift mutations without metabolic activation; a more pronounced effect was seen in its derivative YG1024 which overexpresses N-acetyltransferase. Induction of single/double strand breaks by Comet assay was detected with concentrations > 0.125 mg/mL in liver derived cells; as well as increased rates for micronucleus (reflecting structural and numeric chromosomal aberrations) and nuclear buds which are a consequence of gene amplifications were seen with a higher dose (2.0 mg/mL) (p < 0.05; Tukey's test). The mutational pattern which was observed in the bacterial tests indicates that the cleavage product p-nitroaniline may cause the genotoxic effects of the dye. Our findings indicate that exposure of humans and the release of the compound into the environment may lead to adverse effects due to its DNA damaging activity.