In vivo degradation behavior of enzyme-degradable poly(trimethylene carbonate)-based biohybrid networks of varying water content.

Polymeric biohybrid networks have significant potential as supportive materials for soft connective tissue regeneration. Their success in this regard is determined by their initial mechanical properties, which are dependent on their water content, as well as the rate at which these properties change with time due to cell mediated degradation. In this study the in vivo degradation and tissue response following implantation of matrix metalloproteinase (MMP)-degradable poly(trimethylene carbonate) (PTMC)-based biohybrid networks were assessed in a Wistar rat model. The networks examined varied in equilibrium water content from circa 20% to 70% w/w. The networks degraded through MMP secretion by inflammatory cells at the tissue-material interface, generating a mass loss profile consistent with surface erosion but modulus and sol content changes consistent with a bulk erosion process. This degradation profile was explained in terms of a population gradient in MMP concentration from the surface to the bulk of the networks due to diffusion restrictions. A histological analysis of the tissue surrounding the implants confirmed a moderate tissue response comparable to that observed towards a VicrylTM suture, suggesting that these new materials can be considered biocompatible.

High modulus, enzyme-degradable poly(trimethylene carbonate)-peptide biohybrid networks formed from triblock prepolymers.

Biohybrid networks have the potential to have stiffnesses equivalent to that of native soft connective tissues as well as cell-mediated degradation behavior. Most strategies to generate such materials to date have utilized crosslinking of two separate and orthogonally functionalized polymers. Herein we describe a triblock prepolymer consisting of a central enzyme degradable peptide block flanked by two synthetic, hydrolysis resistant poly(trimethylene carbonate) blocks (PTMC) or poly(ethylene glycol)-PTMC blocks terminated in methacrylate groups. To form these prepolymers heterobifunctional PTMC and PEG-PTMC were prepared, possessing a vinyl sulfone terminus and a methacrylate terminus. These polymers were conjugated to a di-cysteine containing peptide through a Michael-type addition to form cross-linkable prepolymers. These prepolymers were then photo-cured to form enzyme degradable networks. The compressive moduli of the resulting water swollen networks was within the range of many soft connective tissues and was inversely proportional to the water solubility of the prepolymers. The prepolymer water solubility in turn could be tuned by adjusting PTMC molecular weight or by the addition of a PEG block. In vitro degradation only occurred in the presence of matrix metalloproteinases, and was fastest for networks prepared with prepolymers of higher water solubility.

Ultrasonic induced photoluminescence decay in sonochemically obtained cauliflower-like ZnO nanostructures with surface 1D nanoarrays.

Cauliflower-like ZnO nanostructures with average crystallite size of about 55 nm which have surface one dimensional (1D) nanoarrays with 10 nm diameter were successfully fabricated through a simple sonochemical route. X-ray diffractometry (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and room temperature photoluminescence (PL) characterizations were performed to investigate the morphological and structural properties of the obtained nanostructures. It has been shown that the synthesized cauliflower-like ZnO nanostructures irradiated UV luminescence and a green peak in visible band. Ultrasonic post-treatment of the particles for about 2 h increased the density of surface defects resulted in an increase in the green emission intensity.