Annotation of chemotaxis gene clusters and proteins involved in chemotaxis of Bacillus subtilis strain MB378 capable of biodecolorizing different dyes.

This study explores the chemotactic potential of Bacillus subtilis MB378 against industrial dyes. Initial screening with swim plate assay showed significant movement of Bacillus subtilis MB378 towards test compounds. According to quantitative capillary assay, B. subtilis MB378 exhibited high chemotaxis potential towards Acid Orange 52 (CI: 9.52), followed by Direct Red 28 (CI: 8.39) and Basic Green 4 (CI: 5.21) in glucose-supplemented medium. Sequencing and gene annotation results evidently showed presence of chemotaxis genes and flagellar motor proteins in Bacillus subtilis draft genome. Methyl-accepting proteins (involved in chemotaxis regulation) belonged to pfam00672, pfam00072, and pfam00015 protein families. Annotated chemotaxis machinery of MB378 comprised 8 Che genes, 5 chemoreceptor genes, associated flagellar proteins, and rotary motors. Chemotaxis genes of B. subtilis MB378 were compared with genes of closely related Bacillus strains (168, WK1, and HTA426), depicting highly conserved regions showing evolutionary relation between them. Considering results of present study, it can be speculated that test compounds triggered chemotactic genes, which made these compounds bioavailable to the bacterium. Hence, the bacterium recognized and approached these compounds and facilitated biodegradation and detoxification of these compounds.

Decolorization and discovery of metabolic pathway for the degradation of Mordant Black 11 dye by Klebsiella sp. MB398.

Extensive utilization of the synthetic dyes in various industries is leading to water and soil contamination and ultimately impacting the humans. A research study was conducted for investigating the biodecolorization and biotransformation of Mordant Black 11 dye. For this purpose, potential of biofilm forming bacteria Klebsiella pneumoniae MB398 isolated from effluent outlets of Tops Food Industry, Hattar, Pakistan, was assessed to decolorize and transform Mordant Black 11 dye. Bacterial strain MB398 exhibited the capability of growing optimally at acidic pH (pH 6.0). Klebsiella pneumoniae MB398 efficiently decolorized Mordant Black 11 dye (64.55%) in aerobic environment at pH 6.0 and 37 °C over 24 h, which further increased to 75.35% over a period of 72 h of incubation. Strain MB398 also exhibited the capability of decolorizing Mordant Black 11 dye in the presence of cadmium (63.71%), chromium (61.78%), and copper (61.50%), respectively. UV-VIS spectrophotometric analysis, FTIR, and HPLC spectra were also indicative of biotransformation of dye molecules by Klebsiella pneumoniae MB398. GC-MS analysis of Mordant Black 11 dye revealed formation of 9 novel and unique metabolites including phenol,2,4-bis(1,1-dimethylethyl); 9-eicosene, (E); ethanol,2,2-(2-propenyloxy); acetic acid, benzene; 1-naphthol; methyl formate; valeraldehyde,2,4-dimethyl; and 7-hexadecene (Z). A possible metabolic pathway depicting the biotransformation of Mordant Black 11 dye by Klebsiella pneumoniae MB398 was projected. Findings of the current research study strongly suggest application of Klebsiella pneumoniae MB398 for developing large scale bioremediation strategies for the abatement of synthetic dyes to retain environmental sustainability in bioeconomic way.

Concurrent uptake and metabolism of dyestuffs through bio-assisted phytoremediation: a symbiotic approach.

Manipulation of bio-technological processes in treatment of dyestuffs has attracted considerable attention, because a large proportion of these synthetic dyes enter into natural environment during synthesis and dyeing operations that contaminates different ecosystems. Moreover, these dyestuffs are toxic and difficult to degrade because of their synthetic origin, durability, and complex aromatic molecular structures. Hence, bio-assisted phytoremediation has recently emerged as an innovative cleanup approach in which microorganisms and plants work together to transform xenobiotic dyestuffs into nontoxic or less harmful products. This manuscript will focus on competence and potential of plant-microbe synergistic systems for treatment of dyestuffs, their mixtures and real textile effluents, and effects of symbiotic relationship on plant performances during remediation process and will highlight their metabolic activities during bio-assisted phytodegradation and detoxification.

Wolbachia pipientis: A potential candidate for combating and eradicating dengue epidemics in Pakistan.

Dengue virus syndrome is an emerging global health challenge which is endemic in tropical countries like Pakistan. In recent years dengue incidences have increased considerably in different areas of Pakistan with more sever impacts on urban and peri-urban populations. This review is an effort to highlight the changing epidemiology of dengue fever, role of Government of Pakistan in disease management and control using preventive and community based approaches in the region. Moreover, there is an emphasis on application of Wolbachia as novel, inexpensive and environmentally benign candidate for control and eradication of dengue transmitting vectors.