RESUMEN
The compact high-voltage power supply is developed for stimulation to promote fruiting body formation in cultivating L. edodes and Lyophyllum deeastes Sing. mushrooms. A Cockcroft-Walton (C-W) circuit is employed to generate DC high-voltage from AC 100 V plug power for the compact, easy handling and high safety use in the hilly and mountainous area. The C-W circuit is connected to high-voltage coaxial cable which works for high-voltage transmission and for charging up as energy storage capacitor. The output voltage is around 50 kV with several microseconds pulse width. The dimension and weight of the developed power supply are 0.4 × 0.47 × 1 m³ and 8.1 kg, respectively. The effect of the high-voltage stimulation on enhancement of fruiting body formation is evaluated in cultivating L. edodes and Lyophyllum deeastes Sing. mushrooms using the developed compact high-voltage power supply. The conventional Marx generator is also used for comparison in effect of high-voltage stimulation for fruiting body formation. L. edodes is cultivated with hosting to natural logs and the pulsed high voltage is applied to the cultivated natural logs. The substrate for Lyophyllum deeastes Sing. cultivation consists of sawdust. The results show that the fruiting body formation of mushrooms of L. edodes for four cultivation seasons and that of Lyophyllum deeastes Sing. for two seasons both increase approximately 1.3 times higher than control group in terms of the total weight. Although the input energy per a pulse is difference with the generators, the improvement of the fruit body yield mainly depends on the total input energy into the log. The effect for promotion on fruiting body formation by the developed compact high-voltage power supply is almost same that by the conventional Marx generator.
RESUMEN
The effect of high-voltage electrical stimulation on fruit body formation in cultivating mushrooms was evaluated using a compact pulsed power generator designed and based on an inductive energy storage system. An output voltage from 50 to 130 kV with a 100 ns pulse width was used as the electrical stimulation to determine the optimum amplitude. The pulsed high voltage was applied to a sawdust-based substrate of Lyophyllum decastes and natural logs hosting Lentinula edodes, Pholiota nameko, and Naematoloma sublateritium. The experimental results showed that the fruit body formation of mushrooms increased 1.3-2.0 times in terms of the total weight. The accumulated yield of Lentinula edodes for four cultivation seasons was improved from 160 to 320 g by applying voltages of 50 or 100 kV. However, the yield was decreased from 320 to 240 g upon increasing the applied voltage from 100 to 130 kV. The yield of the other types of mushrooms showed tendencies similar to those of Lentinula edodes when voltage was applied. An optimal voltage was confirmed for efficient fruit body induction. The hypha activity was evaluated by the amount of hydrophobin release, which was mainly observed before the fruit body formation. The hydrophobin release decreased for three hours after stimulation. However, the hydrophobin release from the vegetative hyphae increased 2.3 times one day after the stimulation.
